The Globin Composition of Daphnia pulex Hemoglobin

  • Kris Peeters
  • Luc Moens
Conference paper


Although the majority of Crustacea have He as an O2 carrier, extracellular Hbs are mainly found in the hemolymph of the Branchiopoda. Basing our analysis on globin structure and using Vinogradov’s classification, we may distinguish two groups: the multi-domain-multisubunit Hbs found in Anostraca, and the two domain-multisubunit Hbs found in Notostraca, Conchostraca and Cladocera (1). The Hb of Daphnia pulex is a representative of the latter. It has an S 20°,w of 16.4–16.9, an M r of 420,000–470,000, contains one mole heme per M r = 18,000–20,000, and is composed of different globin chains with M r = 31,000–37,000. Limited proteolysis suggests that one globin chain is comprised of two heme-containing O2 binding domains with M r = 17,000 (2). In this work we describe the globin composition of the D. pulex Hb.


Hydrolysis Phenol Urea Acetonitrile Glycine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Vinogradov, S.N. (1985) Comp. Biochem. Physiol. 82B: 1–15.Google Scholar
  2. 2.
    Dangott, L.J. and Terwilliger, R.C. (1980) Comp. Biochem. Physiol. 67B: 301–306.Google Scholar
  3. 3.
    Peeters, K., Mertens, J., Hebert, P. and Moens, L. (1990) Comp. Biochem. Physiol. 97B: 369–381.Google Scholar
  4. 4.
    Dangott, L.J. and Terwilliger, R.C. (1979) Biochim. Biophys. Acta 579: 452–461.PubMedGoogle Scholar
  5. 5.
    Ilan, E., David, M.M. and Daniel, E. (1981) Biochem. 20: 6190–6194.CrossRefGoogle Scholar
  6. 6.
    Wood, E.J. (1980) Essays Biochem. 16: 1–47.PubMedGoogle Scholar
  7. 7.
    Fushitani, K., Matsuura, M.S.A. and Riggs, A.F. (1988) J. Biol. Chem. 263: 6502–6517.PubMedGoogle Scholar
  8. 8.
    Moens, L., Ver Donck, K., De Smet, K., Van Hauwaert, M.-L., van Beeumen, J., Allard, P., Wodak, S. and Trotman, C.N.A. (1989) In Cell and Molecular Biology of Artemia Development, eds. A.H. Warner, T.H. MaeRae and J.C. Bagshaw, 429–438. New York: Plenum Press.Google Scholar
  9. 9.
    Reeck, G. (1970) In Handbook of Biochemistry, ed. H.A. Sober, C121. Cleveland: CRC-Press.Google Scholar
  10. 10.
    Bidlingmeyer, B.A., Cohen, S.A. and Tarvin, T.L. (1984) J. Chromat. 336: 93–104.CrossRefGoogle Scholar
  11. 11.
    Van Fleteren, J.R. and Peeters, K. (1990) J. Biochem. Biophys. Methods 20: 227–235.CrossRefGoogle Scholar
  12. 12.
    Eckerskorn, C. and Lottspeich, F. (1989) Chromatographia 28: 92–94.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1991

Authors and Affiliations

  • Kris Peeters
    • 1
  • Luc Moens
    • 1
  1. 1.Department of BiochemistryUniversity of AntwerpWilrijkBelgium

Personalised recommendations