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Methods for the Detection, Identification, and Enumeration of Microbes

  • David J. Drahos
Part of the Brock/Springer Series in Contemporary Bioscience book series (BROCK/SPRINGER)

Abstract

The greatest challenge a researcher usually faces when designing definitive experiments to measure the dynamics of microbial populations in the phyllosphere is to develop reliable and practical means to detect, identify, or enumerate a particular strain or group of interest in an environmental sample. While a number of sensitive, and often quite sophisticated and elegant, tools now exist for the task, each has advantages and disadvantages which must be fully appreciated and considered prior to settling on a particular strategy. Failure to take this step early can readily result in erroneous conclusions or an unexpected drain on resources and time. This chapter provides an overview of the current methodologies which have been or could be applied to phyllosphere microbiology, and it includes sufficient key references to allow a more comprehensive assessment of applicability to a particular research plan.

Keywords

Nalidixic Acid Polymerase Chain Reaction Method Environmental Microbiology Xanthomonas Campestris Nucleic Acid Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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  • David J. Drahos

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