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Microbial Ecology of Leaves pp 135–157Cite as

Methods for the Detection, Identification, and Enumeration of Microbes

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Part of the book series: Brock/Springer Series in Contemporary Bioscience ((BROCK/SPRINGER))

Abstract

The greatest challenge a researcher usually faces when designing definitive experiments to measure the dynamics of microbial populations in the phyllosphere is to develop reliable and practical means to detect, identify, or enumerate a particular strain or group of interest in an environmental sample. While a number of sensitive, and often quite sophisticated and elegant, tools now exist for the task, each has advantages and disadvantages which must be fully appreciated and considered prior to settling on a particular strategy. Failure to take this step early can readily result in erroneous conclusions or an unexpected drain on resources and time. This chapter provides an overview of the current methodologies which have been or could be applied to phyllosphere microbiology, and it includes sufficient key references to allow a more comprehensive assessment of applicability to a particular research plan.

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Authors
  1. David J. Drahos
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Editors and Affiliations

  1. Department of Plant Pathology Russell Laboratories, University of Wisconsin-Madison, Madison, WI, 53706, USA

    John H. Andrews  & Susan S. Hirano  & 

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© 1991 Springer-Verlag New York Inc.

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Drahos, D.J. (1991). Methods for the Detection, Identification, and Enumeration of Microbes. In: Andrews, J.H., Hirano, S.S. (eds) Microbial Ecology of Leaves. Brock/Springer Series in Contemporary Bioscience. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3168-4_7

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  • DOI: https://doi.org/10.1007/978-1-4612-3168-4_7

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7822-1

  • Online ISBN: 978-1-4612-3168-4

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