Skip to main content
SpringerLink
Log in

Transforming Growth Factors and Ovarian Function

  • Conference paper
Growth Factors in Reproduction

Part of the book series: Serono Symposia, USA ((SERONOSYMP))

  • 43 Accesses

Abstract

Transforming growth factor α (TGFα) is a 50-amino acid polypeptide that interacts with the same receptor as the epidermal growth factor (EGF) (1). It is, therefore, a regulatory molecule capable of affecting the same cell types, including epithelial cells, that respond to EGF (2). Transforming growth factor βs (TGFβs), on the other hand, are 25-kD peptides consisting of two identical 112-amino acid subunits that are synthesized as part of a larger, biologically latent peptide (3–4). They are considered to be part of a more extensive gene family that includes other ovarian effectors, such as inhibin, activin, and mullerian inhibiting substance (5). The cellular effects of TGFβs are multifactorial and are dependent upon other factors in the milieu, but in general, their effect upon epithelial cells is antimitotic (3). Two excellent reviews of the biochemical properties and cellular actions of TGFα and TGFβs are presented elsewhere in this volume.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Marquardt H, Rose TM, Webb NR, et al. Rat transforming growth factor type I: Structure and relation to epidermal growth factor. Science 1984; 223:1079–1082.

    Article  PubMed  CAS  Google Scholar 

  2. Todaro GJ, Fryling C, DeLarco JE. Tranforming growth factors produced by certain human tumor cells: Polypeptides that interact with epidermal growth factor receptors. Proc Natl Acad Sci USA 1980; 77:5158–5162.

    Article  Google Scholar 

  3. Sporn MB, Roberts AB, Wakefield LM, Assoian RK. Transforming growth factor-β: Biological function and chemical structure. Science 1986; 233:532–540.

    Article  PubMed  CAS  Google Scholar 

  4. Wakefield LM, Smith DL, Flanders KC, Sporn MB. Latent transforming growth factor-β from human platelets. J Biol Chem 1988; 263:7646–7654.

    PubMed  CAS  Google Scholar 

  5. Mason AJ, Hayflick JS, Ling N, et al. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with tranforming growth factor-β. Nature 1985; 318:659–663.

    Article  PubMed  CAS  Google Scholar 

  6. Knecht M, Feng P, Catt KJ. Transforming growth factor-beta: Autocrine, paracrine, and endocrine effects in ovarian cells. Seminars Reprod Endocrinol 1989; 7:12–20.

    Article  Google Scholar 

  7. Schomberg DW. Growth factors and reproduction. In: Hodgen GD, Rosenwaks Z, Spieler JM, eds. Nonsteroidal gonadal factors. Norfolk, CT: Jones Institute Press, 1988:330–338.

    Google Scholar 

  8. May JV, Schomberg DW. The potential relevance of epidermal growth factor and transforming growth factor-alpha to ovarian physiology. Seminars Reprod Endocrinol 1989; 7:1–11.

    Article  Google Scholar 

  9. Skinner MK. Transforming growth factor production and action in the ovarian follicle: Thecal cell-granulosa cell interactions. In: Hirshfield AN, ed. Growth factors and the ovary. New York: Plenum Press, 1989:141–150.

    Google Scholar 

  10. Hammond JM. Paracrine regulation in the ovary. In: Medically assisted conception, an agenda for research. Institute of Medicine, National Academy Press, 1989:211–233.

    Google Scholar 

  11. Gospodarowicz D, Mescher AL, Birdwell CR. Control of cellular proliferation by the fibroblast and epidermal growth factors. In: Third decennial review conference: Cell tissue and organ culture. National Cancer Institute Monograph 48. Bethesda, MD: USPHS, NIH, 1978:109–130.

    Google Scholar 

  12. Lintern-Moore S, Moore GPM, Panaretto BA, Robertson D. Follicular development in the neonatal mouse ovary: Effect of epidermal growth factor. Acta Endocrinol (Copenh) 1981; 96:123–126.

    CAS  Google Scholar 

  13. Shaw G, Jorgenson G, Tweendale R, Tennison M, Waters MJ. Effect of epidermal growth factor on reproductive function of ewes. J Endocrinol 1985; 107:429–436.

    Article  PubMed  CAS  Google Scholar 

  14. Radford HM, Avenell JA, Panaretto BA, Some effects of epidermal growth factor on reproductive function in merino sheep. J Reprod Fertil 1987; 80:113–118.

    Article  PubMed  CAS  Google Scholar 

  15. Richards JS. Estradiol receptor content in rat granulosa cells during follicular development: Modification by estradiol and gonadotropins. Endocrinology 1975; 97:74–84.

    Article  Google Scholar 

  16. Downs SM. Specificity of epidermal growth factor action on maturation of the murine oocyte and cumulus oophorus in vitro. Biol Reprod 1989; 41:371–379.

    Article  PubMed  CAS  Google Scholar 

  17. Feng P, Catt KJ, Knecht M. Transforming growth factor-β stimulates meiotic maturation of the rat oocyte. Endocrinology 1988; 122:181–188.

    Article  PubMed  CAS  Google Scholar 

  18. Tsafrirri A, Vale W, Hsueh AJW. Effects of transforming growth factors and inhibin-related proteins on rat preovulatory graafian follicles in vitro. Endocrinology 1989; 125:1857–1862.

    Article  Google Scholar 

  19. Galaway AB, Oikawa M, Ny T, Hsueh AJW. Epidermal growth factor stimulates tissue plasminogen activator activity and messenger ribonucleic acid levels in cultured rat granulosa cells: Mediation by pathways independent of protein kinases A and C. Endocrinology 1989; 125:126–135.

    Article  Google Scholar 

  20. Gospodarowicz D, Plouet J, Fugii DK. Ovarian germinal epithelial cells repond to basic fibroblast growth factor and express its gene: Implications for early folliculogenesis. Endocrinology 1989; 125:1266–1276.

    Article  PubMed  CAS  Google Scholar 

  21. Magoffin DA, Gancedo B, Erickson GF. Transforming growth factor-β promotes differentiation of ovarian thecal-interstitial cells but inhibits androgen production. Endocrinology 1989; 125:1951–1958.

    Article  PubMed  CAS  Google Scholar 

  22. Caubo B, DeVinna RS, Tonetta SA. Regulation of steroidogenesis in cultured porcine granulosa cells by growth factors. Endocrinology 1989; 125:321–326.

    Article  PubMed  CAS  Google Scholar 

  23. Mendelson CR, Means GD, Mahendroo MS, et al. Use of molecular probes to study regulation of aromatase cytochrome P-450. Biol Reprod 1990; 42:1–10.

    Article  PubMed  CAS  Google Scholar 

  24. Hsueh AJW, Adashi EY, Jones PCB, Walsh TH Jr. Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocr Rev 1984; 5:76–127.

    Article  PubMed  CAS  Google Scholar 

  25. Gospodarowicz D, Bialecki H. Fibroblast and epidermal growth factors are mitogenic for cultured granulosa cells of rodent, porcine, and human origin. Endocrinology 1979; 5:76–127.

    Google Scholar 

  26. Dorrington J, Chuma AV, Bendali JJ. Transforming growth factor β and follicle-stimulating hormone promote rat granulosa cell proliferation. Endocrinology 1988; 123:353–359.

    Article  PubMed  CAS  Google Scholar 

  27. Gospodarowicz D. The control of proliferation of ovarian cells by the epidermal and fibroblast growth factors. In: Spilman CH, Wilks JW, eds. Novel aspects of reproductive physiology. New York: SP Medical and Scientific Books, 1978:107–180.

    Google Scholar 

  28. Hirshfield AN, Schmidt WA. Kinetic aspects of follicular development in the rat. In: Mahesh VB, Dhindsa DS, Anderson E, Kalra SP, eds. New York: Plenum Press, 1987; 211–236.

    Google Scholar 

  29. Delidow BC, White BA, Peluso JJ. Gonadotropin induction of c-fos and c-myc expression and deoxyribonucleic acid synthesis in rat granulosa cells. Endocrinology 1990; 126:2302–2306.

    Article  PubMed  CAS  Google Scholar 

  30. Mondschein JS, Hammond JM. Growth factors regulate immunoreactive insulin-like growth factor-I production by cultured porcine granulosa cells. Endocrinology 1988; 123:463–468.

    Article  PubMed  CAS  Google Scholar 

  31. Mondschein JS, Canning SF, Hammond JM. Effects of transforming growth factor-β on the production of immunoreactive insulin-like growth factor I and progesterone and on [3H-T]thymidine incorporation in porcine granulosa cell cultures. Endocrinology 1988; 123:1970–1976.

    Article  PubMed  CAS  Google Scholar 

  32. Skinner MK, Coffey RJ Jr. Regulation of ovarian cell growth through the local production of transforming growth factor-α by thecal cells. Endocrinology 1988; 123:2632–2638.

    Article  PubMed  CAS  Google Scholar 

  33. Lobb DK, Skinner MK, Dorrington JH. Rat thecal/interstitial cells produce a mitogenic activity that promotes the growth of granulosa cells. Mol Cell Endocrinol 1988; 55:209–217.

    Article  PubMed  CAS  Google Scholar 

  34. Bendell JJ, Lobb DK, Chuma A, Gysler M, Dorrington JH. Bovine thecal cells secrete a factor(s) that promotes granulosa cell proliferation. Biol Reprod 1988; 38:79–97.

    Article  Google Scholar 

  35. Kudlow JE, Korbin MS, Purchio AF, et al. Ovarian transforming growth factor-α gene expression: Immunohistochemical localization to the thecal/interstitial cells. Endocrinology 1987; 121:1577–1579.

    Article  PubMed  CAS  Google Scholar 

  36. Lobb DK, Korbin MS, Kudlow JE, Dorrington JH. Transforming growth factor-alpha in the adult bovine ovary: Identification in growing ovarian follicles. Biol Reprod 1989; 40:1087–1093.

    Article  PubMed  CAS  Google Scholar 

  37. Roy SK, Greenwald GS. Immunohistochemical localization of epidermal growth factor-like activity in the hamster ovary with a polyclonal antibody. Endocrinology 1990; 126:1309–1317.

    Article  PubMed  CAS  Google Scholar 

  38. Hernandez ER, Twardzik DR, Purchio A, Adashi EY. Gonadotropin-dependent ovarian transforming growth factor-β gene expression [Abstract 35]. Biol Reprod 1987; 1 (suppl).

    Google Scholar 

  39. Thompson NL, Flanders KC, Smith JM, Ellingsworth LR, Roberts AB, Sporn MB. Expression of transforming growth factor-β1 in specific cells and tissues of adult and neonatal mice. J Cell Biol 1989; 108:661–669.

    Article  PubMed  CAS  Google Scholar 

  40. Knecht M, Feng P, Catt K, Gelmann E. Expression of the TGF-β gene in the ovary and its role during meiosis of rat oocytes [Abstract D113]. J Cell Biochem 1988.

    Google Scholar 

  41. Gaddy-Kurten D, Hickey GJ, Fey GH, Gouldie J, Richards JS. Hormonal regulation and tissue-specific localization of a-2 macroglobulin in rat ovarian follicles and corpora lutea. Endocrinology 1989; 125:2985–2995.

    Article  PubMed  CAS  Google Scholar 

  42. Skinner MK, Keski-Oja J, Osteen KG, Moses HL. Ovarian thecal cells produce transforming growth factor-β which can regulate granulosa cell growth. Endocrinology 1987; 121:786–792.

    Article  PubMed  CAS  Google Scholar 

  43. Kim I-C, Schomberg DW. The production of transforming growth factor-β activity by rat granulosa cells. Endocrinology 1989; 124:1345–1351.

    Article  PubMed  CAS  Google Scholar 

  44. Mulheron GW, Schomberg DW. Rat granulosa cells express transforming growth factor-β type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro. Endocrinology 1990; 126:1777–1779.

    Article  PubMed  CAS  Google Scholar 

  45. Glick AB, Flanders KC, Danielpour D, Yuspa SH, Sporn MB. Retionic acid induces transforming growth factor-β2 in cultured keratinocytes and mouse epidermis. Cell Regulation 1989; 1:87–97.

    PubMed  CAS  Google Scholar 

  46. Komm BS, Terpening CM, Benz DJ, et al. Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science 1988; 241:81–84.

    Article  PubMed  CAS  Google Scholar 

  47. Ikeda T, Toubin MN, Marquardt H. Human transforming growth factor type B2; production by a prostatic adenocarcinoma cell line, purification and initial characterization. Biochemistry 1987; 26:4337–4345.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA

    David W. Schomberg & George W. Mulheron

  2. Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA

    David W. Schomberg & George W. Mulheron

  3. The Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina, USA

    David W. Schomberg & George W. Mulheron

Authors
  1. David W. Schomberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. George W. Mulheron
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Duke University Medical Center, Durham, NC, 27710, USA

    David W. Schomberg PhD

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag New York, Inc.

About this paper

Cite this paper

Schomberg, D.W., Mulheron, G.W. (1991). Transforming Growth Factors and Ovarian Function. In: Schomberg, D.W. (eds) Growth Factors in Reproduction. Serono Symposia, USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3162-2_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-3162-2_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7819-1

  • Online ISBN: 978-1-4612-3162-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation