Filtration and Buoyant Density Characterization of Algal Alkaline Phosphatase

  • David A. Francko
Part of the Brock/Springer Series in Contemporary Bioscience book series (BROCK/SPRINGER)

Abstract

Many planktonic algae and bacteria respond to acute orthophosphate limiting conditions by synthesizing alkaline phosphatase (APase). This class of enzymes permits biota to hydrolyze dissolved phosphomonoester substrates present in the water column, thereby providing an additional source of orthophosphate for biotic assimilation (Berman, 1970; Heath and Cooke, 1975; Francko and Heath, 1979; Pettersson, 1980; Wetzel, 1981; Heath, 1986; Francko, 1986a). As such, APase plays a central role in the overall process of phosphorus recycling and orthophosphate regeneration in epilimnetic waters (reviewed by Franko, 1986a).

Keywords

Sucrose Phosphorus Filtration Chlorophyll Phytoplankton 

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References

  1. Berman, T. 1970. Alkaline phosphatases and phosphorus availability in Lake Kinneret. Limnology and Oceanography 15: 663–674.CrossRefGoogle Scholar
  2. Cembella, A.D., Antia, N.J. and P.J. Harrison. 1984. The utilization of inorganic and organic phosphorus compounds as nutrients by eukaryotic microalgae: a multidisciplinary perspective. CRC Critical Reviews in Microbiology 10: 317–391.PubMedCrossRefGoogle Scholar
  3. Francko, D.A. 1983. Size-fractionation of alkaline phosphatase activity in lake water by membrane filtration. Journal of Freshwater Ecology 2: 305–309.CrossRefGoogle Scholar
  4. Francko, D.A. 1986a. Epilimnetic phosphorus cycling: influence of humic materials and iron on co-existing major mechanisms. Canadian Journal of Fisheries and Aquatic Sciences 43: 302–310.CrossRefGoogle Scholar
  5. Francko, D.A. 1986b. Measurement of algal chlorophyll. and carbon assimilation by a tissue solubilizer method: A critical analysis. Archiv für Hydrobiologie 106: 327–335.Google Scholar
  6. Francko, D.A. and R.T. Heath. 1979. Functionally distinct classes of complex phosphorus compounds in lake water. Limnology and Oceanography 24: 463–473.CrossRefGoogle Scholar
  7. Heath, R.T. 1986. Dissolved organic phosphorus compounds: do they satisfy planktonic phosphate demand in summer? Canadian Journal of Fisheries and Aquatic Sciences 43: 343–350.CrossRefGoogle Scholar
  8. Heath, R.T. and G.D. Cooke. 1975. The significance of alkaline phosphatase in a eutrophic lake. Verhandlungen der Internationalen Vereinigung für Theoretische and Angewandte Limnologie 19: 959–965.Google Scholar
  9. Heath, R.T. and D.A. Francko. 1988. Comparison of phosphorus dynamics in two Oklahoma reservoirs and a natural lake varying in abiogenic turbidity. Canadian Journal of Fisheries and Aquatic Sciences 45: 1480–1486.CrossRefGoogle Scholar
  10. Moss, B. 1972. The influences of environmental factors on the distribution of freshwater algae: an experimental study. I. Introduction and the influence of calcium concentration. Journal of Ecology 60: 917.CrossRefGoogle Scholar
  11. Perry, M.H. 1972. Alkaline phosphatase activity in subtropical Central North Pacific waters using a sensitive fluorometric method. Marine Biology 15: 109–113.CrossRefGoogle Scholar
  12. Pettersson, K. 1980. Alkaline phosphatase activity and algal surplus phosphorus as phosphorus-deficiency indicator in Lake Erken. Archiv für Hydrobiology 89: 54–87.Google Scholar
  13. Stewart, A.J. and R.G. Wetzel. 1982. Phytoplankton contribution to alkaline phosphatase activity. Archiv für Hydrobiologie 93: 265–271.Google Scholar
  14. Wetzel, R.G. 1982. Longterm dissolved and particulate alkaline phosphatase activity in a hardwater lake in relation to lake stability and phosphorus enrichments. Verhandlungen der Internationalen Vereinigung für Theoretische and Angewandte Limnologie 21: 337–349.Google Scholar
  15. Wollum, A.G. and R.H. Miller. 1980. Density centrifugation method for recovering Rhizobium spp. from soil for fluorescent-antibody studies. Applied and Environmental Microbiology 39: 466–469.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • David A. Francko

There are no affiliations available

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