Abstract
We are isolating and studying genes required for pathogenicity of Ustilago maydis, the causative agent of corn smut disease (Christensen, 1963). This phytopathogenic basidiomycete offers an attractive system in which to gain a molecular understanding of host-parasite interactions. The organism grows as a haploid yeast on defined laboratory media, mutants are readily generated by UV or chemical mutagenesis, stable diploids can be constructed for mitotic recombination and complementation analysis, and the fungus is amenable to Mendelian genetic analysis (Holliday, 1974). These attributes are not found in combination in any other phytopathogenic fungus. Although this pathogen is no longer a production constraint in North America, where resistant hybrid corn is grown, it continues to be a problem in third world countries where susceptible varieties are still cultivated. Moreover, we hope that an understanding of mechanisms of pathogenesis in this host-parasite interaction will have application to more economically important and difficult to study fungal diseases such as the bunts and rusts. We have initiated a molecular genetic analysis of two gene systems thought to control pathogenic growth of U. maydis in maize. These include genes that program sexual development and genes involved in the high affinity, siderophore-mediated iron uptake system of the fungus. In order to conduct these analyses, one of our first goals has been to develop tools which enable us to clone, study, and transfer genes in U. maydis.
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References
Banuett, F. and Herskowitz, I., 1989, Different a alleles of Ustilago maydis are necessary for maintenance of filamentous growth but not for meiosis, Proc. Natl. Acad. Sci. USA 86:5878–5882.
Banks, G.R. and Taylor, S.Y., 1988, Cloning of the PYR3 gene of Ustilago maydis and its use in DNA transformation. Mol. Cell. Biol, 8:5417–5424.
Budde, A.D. and Leong, S.A., 1989, Characterization of siderophores from Ustilago maydis, Mycopathologia 108:125–133.
Budde, A.D. and Leong, S.A., 1990, Ustilago maydis. In Genetic Maps, vol. 5 (S.J. O’Brien, ed.), Cold Spring Harbor Laboratory, New York.
Callow, J. A. and Ling, LT., 1973, Histology of neoplasms and chlorotic lesions in maize seedlings following the injection of sporidia of Ustilago maydis (DC) Corda. Physiol. Plant Pathol. 3:489–494.
Christensen, J.J., 1963, Corn smut caused by Ustilago maydis, Am. Phytopathol. Soc. Mono.
Day, P.R., Anagnostakis, S.L. and Puhalla, J.E., 1971, Pathogenicity resulting from mutation at the b locus of U. maydis, Proc. Natl. Acad. Sci. USA 68:533–535.
Fotheringham, S. and Holloman, W.K., 1989, Cloning and disruption of Ustilago maydis genes, Mol. Cell. Biol. 9:4052–4055.
Froeliger, E.H. and Kronstad, J.W., 1990, Mating and pathogenicity in Ustilago maydis. In Seminars in Developmental Biology: Fungal Development and Mating Interactions (C.A. Raper and D.I. Johnson, eds.), Saunders Scientific Publications, London, (in press)
Froeliger, E.H., and Leong, S.A., 1989, Isolation of mating type genes from Ustilago maydis. Genetics and Cellular Biology of Basidiomycetes (Abstr.). University of Toronto Press, Toronto pp. 25.
Giasson, L. et al., 1989, Cloning and comparison of Aα mating-type alleles of the basidiomycete Schizophyllum commune, Mol. Gen. Genet. 218:72–77.
Glass, N.L. et al., 1988, DNAs of the two mating type alleles of Neurospora crassa are highly dissimilar, Science 241:570–573.
Herskowitz, I., 1988, Life cycle of the budding yeast Saccharomyces cerevisiae, Microbiol. Rev. 52:536–553.
Holden, D., Wang, J. and Leong, S.A., 1988, DNA-mediated transformation of Ustilago hordei and Ustilago nigra, Physiol. Mol. Plant Pathol. 33:235–239.
Holden, D.W., Kronstad, J. and Leong, S.A., 1989, Mutation in a heat-regulated hsp70 gene of Ustilago maydis, EMBO J. 8:1927–1934.
Holliday, R., 1974, Ustilago maydis, In Handbook of Genetics, vol. 1 (King, R.C., ed.) Plenum Press, New York. pp. 575–595.
Kinscherf, T., and Leong, S.A., 1988, Molecular analysis of the karyotype of Ustilago maydis, Chromosoma 96:427–433.
Kronstad, J.W., et al., 1989, Isolation of metabolic genes and demonstration of gene disruption in Ustilago maydis, Gene 79:97–106.
Kronstad, J.W. and Leong, S.A., 1989a, Isolation of two alleles of the b locus of Ustilago maydis, Proc. Natl. Acad. Sci. USA 86:978–982.
Kronstad, J.W. and Leong, S.A., 1989b, Molecular characterization of the b locus of Ustilago maydis. Fungal Genet. Newsl. 36:26.
Kronstad, J.W. and Leong, S.A., 1990, The b mating type forms of Ustilago Maydis contains variable and constant domains. Genes Dev. 4:1384–1395.
Leong, S.A., 1987, Molecular strategies for the analysis of the interaction of Ustilago maydis and maize. In Molecular Strategies for Crop Protection (Arntzen, C, and Ryan, C. eds.), Alan R. Liss, New York, pp. 95–106.
Leong, S.A., 1988, Identification and molecular characterization of genes which control pathogenic growth of Ustilago maydis in maize. In Molecular Genetics of Plant-Microbe Interactions (Verma, D.P. and Palacios, R. eds.), APS Press. St. Paul. pp. 241–246.
Leong, S.A. and Expert, D., 1989, Siderophores in plant-pathogen interactions. In Plant Microbe Interactions-A Molecular Genetic Perspective, vol. 3 (Nester, E. and Kosuge, T., eds.), McGraw-Hill, New York, pp. 62–83.
Mills, L.J., and Kotze, J.M., 1981, Scanning electron microscopy of the germination, growth and infection of Ustilago maydis on maize, Phytopath. Z. 102:21–27.
Puhalla, J.E., 1968, Compatibility reactions on solid medium and interstrain inhibition in Ustilago maydis, Genetics 60: 461–474.
Samac, D.A., and Leong, S.A., 1989, Characterization of the termini of linear plasmids from Nectria haematoccoca and their use in construction of an autonomously replicating transformation vector, Curr. Genet. 16:187–194.
Schulz, B., et al., 1990, The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif, Cell 60:295–306.
Smith, T., and Leong, S.A., 1990, Structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Ustilago maydis, Gene. (in press)
Tsukuda, T., et al., 1988, Isolation and characterization of an automonously replicating sequence from Ustilago maydis, Mol. Cell. Biol. 8:3703–3709.
Turgeon, G., 1989, Molecular Analyses of the mating type locus of Cochliobolus heterostrophus. Fungal. Genet. Newsl. #36 (Peter J. Russel, ed) p. 6.
Walter, J.M., 1933, The mode of entrance of Ustilago zeae into corn, Phytopathology 24:1012–1020.
Wang, J., Holden, D., and Leong, S.A., 1988, Gene transfer system for the phytopathogenic fungus Ustilago maydis, Proc. Natl. Acad. Sci. USA 85:865–869.
Wang, J., Budde, A., and Leong, S.A., 1989, Analysis of ferrichrome biosynthesis in the phytopathogenic fungus Ustilago maydis: cloning of an ornithine-N5-oxygenase gene, J. Bacteriol. 171:2811–2818.
Wang, J., and Leong, S.A., 1989, DNA-mediated transformation of phytopathogenic fungi. In Genetic Engineering, Principles and Practice, (Setlow, J. ed.), Plenum Press, New York, vol. 11 pp. 127–143.
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Leong, S.A. et al. (1991). Molecular Analysis of Pathogenesis in Ustilago maydis . In: Patil, S.S., Mills, D., Ouchi, S., Vance, C. (eds) Molecular Strategies of Pathogens and Host Plants. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3084-7_9
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DOI: https://doi.org/10.1007/978-1-4612-3084-7_9
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