Abstract
Much information about gibberellin (GA) biosynthesis in higher plants has come from studies using cell-free systems prepared from immature seeds (for a recent review, see Graebe1). Cell-free systems from immature seeds of Phaseolus vulgaris catalyze the conversion of GA20 to GA1, GA5, and GA6 by 3β-hydroxylation, 2,3-dehydrogenation, and 2,3-epoxidation of GA20, respectively.2–5 Of these reactions, 3β-hydroxylation is particularly interesting because it has been suggested that GA1 is the active GA in the regulation of internode elongation in maize,6 pea,7 and bean.8 Although the physiological function of GAs in immature seeds is unknown, immature seed is a convenient material for the isolation of the enzyme, 3β-hydroxylase.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Graebe JE. Gibberellin biosynthesis and control. Ann Rev Plant Physiol. 1987; 38: 419–465.
Kamiya Y, Takahashi M, Takahashi N, et al. Conversion of gibberellin A20 to giberellins Al and A5 in a cell-free system from Phaseolus vulgaris Planta. 1984; 162: 154–158.
Takahashi M, Kamiya Y, Takahashi N, et al. Metabolism of gibberellins in a cell-free system from immature seeds of Phaseolus vulgaris L. Planta. 1986; 168: 190–199.
Kwak S-S, Kamiya Y, Takahashi M, et al. Metabolism of [14C]GA20 in a cell-free system from developing seeds of Phaseolus vulgaris L. Plant Cell Physiol. 1988; 29: 707–711.
Albone K, Gaskin P, MacMillan J, et al. Enzymes from seeds of Phaseolus vulgaris L.:Hydroxylation of gibberellin A20 and Al and 2,3-dehydrogenation of gibberellin A20. Planta. 1989; 177: 108–115.
Spray C, Phinney BO, Gaskin P, et al. Internode length in Zea mays L. The dwarf-lmutant controls the 3/3-hydroxylation of gibberellin A20 to gibberellin Al. Planta. 1984; 160: 464–468.
Ingram TJ, Reid JB, Gaskin P, et al. Internode length in Pisum. The Le gene controls the 3ß-hydroxylation of gibberellin A20 to gibberellin Al. Planta. 1984; 160: 454–463.
Endo K, Yamane H, Nakayama M, et al. Endogenous gibberellins in the vegetative shoots of tall and dwarf cultivars of Phaseolus vulgaris L. Plant Cell Physiol. 1989; 30: 137–142.
Kwak S-S, Kamiya Y, Sakurai A, et al. Partial purification and characterization of gibberellin 3/3-hydroxylase from immature seeds of Phaseolus vulgaris L. Plant Cell Physiol. 1988; 29: 935–943.
Hedden P, Graebe JE. Cofactor requirements for the soluble oxidases in the metabolism of the C20-gibberellins. J Plant Growth Regul. 1982; 1: 105–116.
Hashimoto T. Gibberellin structure-dependent interaction between gibberellins and deoxygibberellin C in the growth of dwarf maize seedlings. Plant Physiol. 1987; 83: 910–914.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag New York Inc.
About this paper
Cite this paper
Kamiya, Y., Kwak, SS. (1991). Partial Characterization of the Gibberellin 3β-Hydroxylase from Immature Seeds of Phaseolus vulgaris . In: Takahashi, N., Phinney, B.O., MacMillan, J. (eds) Gibberellins. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3002-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3002-1_8
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-7754-5
Online ISBN: 978-1-4612-3002-1
eBook Packages: Springer Book Archive