Abstract
Cell-free preparations of Cucurbita maxima endosperm have been used for two decades to investigate gibberellin (GA) biosynthesis. The pathway as originally defined in this system has been described in several recent reviews.1–4 This paper reports new properties of the system that have been uncovered only recently by using the conditions optimal for a partially purified GA C-20 hydroxylase from pea cotyledons, and also by using GA53 instead of GA12 as a substrate. We feel that we are keeping up a tradition in reporting these results here, since it was in Tokyo that a paper with the title “Gibberellin Biosynthesis: New Intermediates in the Cucurbita System” was presented sixteen years ago.5 We will also report on the inhibition of the 2-oxoglutarate-dependent enzymes by a new plant growth retardant and on the properties of a purified C-20 hydroxylase.
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Graebe, J.E., Lange, T., Pertsch, S., Stockl, D. (1991). The Relationship of Different Gibberellin Biosynthetic Pathways in Cucurbita maxima Endosperm and Embryos and the Purification of a C-20 Oxidase from the Endosperm. In: Takahashi, N., Phinney, B.O., MacMillan, J. (eds) Gibberellins. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3002-1_6
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DOI: https://doi.org/10.1007/978-1-4612-3002-1_6
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