Abstract
An immunoassay for gibberellins (GAs) was first reported by Fuchs and Fuchs in 1969.1 In this first report, modified bacteriophage T4 was used as a tracer, and several nanograms of GA3 were required for detection. However, this immunoassay procedure did not attract the attention of GA scientists because it provided less information than combined gas chromatography-mass spectrometry (GC-MS) and insufficient information was available on the specificities of the antiserum. At that time, improvements in GC-MS were making it possible to detect submicrograms of GAs, and GC-MS was more conclusive as well as informative. The mass spectra of all known GAs were stored in MacMillan’s (Bristol) and Takahashi’s (Tokyo) laboratories for consultation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fuchs S, Fuchs Y. Immunological assay for plant hormones using specific antibodies to indoleacetic acid and gibberellic acid. Biochim Biophys Acta. 1969; 192: 528–530.
Weiler EW, Wieczorek U. Determination of femtomol quantities of gibberellic acid by radioimmunoassay. Planta. 1981; 152: 159–167.
Atzorn R, Weiler EW. The immunoassay of gibberellins. II. Quantification of GA3, GA4 and GA7 by ultra-sensitive solid-phase enzyme immunoassays. Planta. 1983; 159: 7–11.
Atzorn R, Weiler EW. The immunoassay of gibberellins. I. Radioimmunoassay for the gibberellins A1, A3, A4, A7, A9 and A20. Planta. 1983; 159: 1–6.
Kurogochi S, Yamaguchi I, Murofushi N, et al. A radioimmunoassay for the dimethyl esters of GA24 and GA19. Phytochemistry. 1987; 26: 2895–2900.
Yamaguchi I, Nakazawa H, Nakagawa R, et al. submitted to Plant Cell Physiol 1990.
Nakajima M, Yamaguchi I, Kizawa S, et al. submitted to Plant Cell Physiol 1990.
Yamaguchi I, Nakagawa R, Kurogochi S, et al. Radioimmunoassay of gibberellins A5 and A20. Plant Cell Physiol. 1987; 28: 815–824.
Yamaguchi I, Nakagawa R, Nakazawa H, et al. to be submitted to Agric. Biol. Chem. 1990.
Oden PC, Heide, OM. Detection and identification of gibberellins in extracts of Begonia leaves by bioassay, radioimmunoassay and gas chromatography-mass spectrometry. Physiol Plant. 1988; 73: 445–450.
Eberle J, Yamaguchi I, Nakagawa R, et al. Monoclonal antibodies against GA13-imide recognize the endogenous plant growth regulator, GA4, and related gibberellins. FEBS Lett. 1986; 202: 27–31.
Knox JP, Beale MH, Butcher GW, et al. Preparation and characterization of monoclonal antibodies which recognise different gibberellin epitopes. Planta. 1987; 170: 86–91.
Knox JP, Beale MH, Butcher GW, et al. Monoclonal antibodies to 13-deoxygibberellins. Plant Physiol. 1988; 88:959–960:
Nakajima M, Yamaguchi I, Nagatani A, et al. submitted to Plant Cell Physiol. 1990.
Beale MH, Hooley R, MacMillan J. Gibberellins: Structure-activity relationship and the design of molecular probes. In: Bopp M, ed. Plant growth substances 1985. Heidelberg: Springer-Verlag, 1986: p. 65–73.
Hum BAL, Chantier SM. Production of reagent antibodies. In: Vunakis HV, Langone JL, ed. Methods in enzymology, Vol. 70, Immunological techniques, Part A. London Academic Press, 1980: p. 105–142.
Pengelly WL. Validation of immunoassays. In: Bopp M, ed. Plant growth substances 1985. Heidelberg: Springer-Verlag, 1986: p. 35–43.
Fuchs Y, German E. Insoluble antibody column for isolation and quantitative determination of gibberellins. Plant Cell Physiol. 1974; 15: 629–633.
Durley RC, Sharp CR, Maki SL, et al. Immunoaffinity techniques applied to the purification of gibberellins from plant extracts. Plant Physiol. 1989; 90: 445451.
Smith VA, MacMillan J. An immunological approach to gibberellin purification and quantification. Plant Physiol. 1989; 90: 1148–1155.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag New York Inc.
About this paper
Cite this paper
Yamaguchi, I., Weiler, E.W. (1991). A Minireview on the Immunoassay for Gibberellins. In: Takahashi, N., Phinney, B.O., MacMillan, J. (eds) Gibberellins. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3002-1_15
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3002-1_15
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-7754-5
Online ISBN: 978-1-4612-3002-1
eBook Packages: Springer Book Archive