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A Minireview on the Immunoassay for Gibberellins

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Gibberellins

Abstract

An immunoassay for gibberellins (GAs) was first reported by Fuchs and Fuchs in 1969.1 In this first report, modified bacteriophage T4 was used as a tracer, and several nanograms of GA3 were required for detection. However, this immunoassay procedure did not attract the attention of GA scientists because it provided less information than combined gas chromatography-mass spectrometry (GC-MS) and insufficient information was available on the specificities of the antiserum. At that time, improvements in GC-MS were making it possible to detect submicrograms of GAs, and GC-MS was more conclusive as well as informative. The mass spectra of all known GAs were stored in MacMillan’s (Bristol) and Takahashi’s (Tokyo) laboratories for consultation.

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© 1991 Springer-Verlag New York Inc.

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Yamaguchi, I., Weiler, E.W. (1991). A Minireview on the Immunoassay for Gibberellins. In: Takahashi, N., Phinney, B.O., MacMillan, J. (eds) Gibberellins. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3002-1_15

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  • DOI: https://doi.org/10.1007/978-1-4612-3002-1_15

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7754-5

  • Online ISBN: 978-1-4612-3002-1

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