Pollen Mediated Indirect Gene Transfer to Dicots and Monocots

  • Dieter Hess
  • Markus Iser Angela Schmid
  • Sabine Stegmaier
  • Klaus Dressler
Conference paper

Abstract

Scientists will often be well advised to follow Nature as close as possible. Therefore, Hess proposed as early as 1974 to use Nature’s masterly elaborated pollen transfer system in introducing exogenous gene material into higher plants. Beginning with the same year (Hess et al., 1974), in the following two decades experimental data on phenotypical, biochemical and formal genetic levels could be obtained, which could be interpreted with a pollen mediated gene transfer. In most of these investigations, however, no molecular proof of gene transfer could be achieved as suitable methods were not available. The only exception were attempts to transfer an E.coli transferase (E.C.2.7.7.12) gene to Petunia: Using Southern blot analysis, uptake and integration of transferase DNA into Petunia DNA could be demonstrated. The limited amount of DNA available from earlier experiments, however, allowed cleavage using only one endonuclease so that a correct restriction analysis was not possible (Hess, 1986, Hess and Dressier, unpublished data). To summarize: in all these early investigations convincing molecular evidence for a gene transfer could not be achieved (review Hess, 1987).

Keywords

HPLC Maize Agar Germinate Flavonoid 

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Copyright information

© Springer-Verlag New York, Inc. 1992

Authors and Affiliations

  • Dieter Hess
  • Markus Iser Angela Schmid
  • Sabine Stegmaier
  • Klaus Dressler

There are no affiliations available

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