Molecular Interactions between Human Rhinoviruses and the Adhesion Receptor ICAM-1

  • Richard J. Colonno
Conference paper


Human rhinoviruses (HRVs), members of the Picornaviridae, are the major causative agents of one of the more elusive diseases known to man, namely the common cold (Gwaltney, 1982). To date, there are 102 recognized serotypes that have been isolated and shown to be antigenically distinct (Hamparian et al., 1987). HRVs are non-enveloped viruses that contain four non-glycosylated structural proteins, designated VP1, VP2, VP3, and VP4, which form a protein capsid with icosahedral symmetry. Within the viral capsid lies a single-stranded genome RNA which serves as a monocistronic mRNA for the synthesis of the 4 structural and 7 nonstructural proteins of the virus. Upon entry into a cell, the RNA genome is translated into a large polyprotein which is subsequently cleaved by two viral proteases encoded within the polyprotein (Palmenberg, 1987). The genome RNA of picornaviruses contains all the information needed to initiate a viral infection since transfection of cells with the genome RNA alone will result in the production of infectious progeny virus (Mizutani et al., 1985).


Sialic Acid Neural Cell Adhesion Molecule Cellular Receptor Group Virus Virus Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abraham, G., and Colonno, R.J., 1983, Many rhinovirus serotypes share the same cellular receptor, J. Virol. 51:340–345.Google Scholar
  2. Abraham, G., and Colonno, R.J., 1988, Characterization of human rhinoviruses displaced by an anti-receptor monoclonal antibody, J Virol. 62:2300–2306.PubMedGoogle Scholar
  3. Chow, M., et al., 1987, Myristylation of Picornavirus capsid protein VP4 and its structural significance, Nature (London) 327:482–486.CrossRefGoogle Scholar
  4. Colonno, R.J., 1987, Cell surface receptors for picornaviruses, BioEssays 5:270–274.CrossRefGoogle Scholar
  5. Colonno, R.J., Callahan, P.L., and Long, W.J., 1986, Isolation of a monoclonal antibody that blocks attachment of the major group of human rhinoviruses, J Virol. 57:7–12.PubMedGoogle Scholar
  6. Colonno, R.J., Tomassini, J.E., and Callahan, P.L., 1987, Isolation and characterization of a monoclonal antibody which blocks attachment of human rhinovirus, In: Positive Strand RNA Viruses (Eds. M.A. Brinton and R. Rueckert) Vol 54:93–102. Google Scholar
  7. Colonno, R.J., et al., 1988, Evidence for the direct involvement of the rhinovirus canyon in receptor binding, Proc. Natl. Acad. Sci USA 85:5449–5453.PubMedCrossRefGoogle Scholar
  8. Colonno, R.J., et al., 1990, The major-group rhinoviruses utilize the intercellular adhesion molecule 1 ligand as a cellular receptor during infection, In: New Aspects of Positive-Strand RNA Viruses (Eds. M.A. Brinton & F.X. Heinz) 257–261.Google Scholar
  9. Dustin, M.L., Staunton, D.W., and Springer, T.A., 1988, Supergene families meet in the immune system, Immunol Today 9:213–215.PubMedCrossRefGoogle Scholar
  10. Giranda, V.L., Chapman, M.S., and Rossmann, M.G., 1990, Modeling of the human intercellular adhesion molecule-1, the human rhinovirus major group receptor, In: Proteins: Structure, Function, and Genetics 7:227–233.CrossRefGoogle Scholar
  11. Greve, J.M., et al., 1989, The major human rhinovirus receptor is ICAM-1, Cell 56:839–847.PubMedCrossRefGoogle Scholar
  12. Gwaltney, J.M., Jr., 1982, Rhinoviruses, In: Viral Infection of Man: Epidemiology and Control (ed. E.A. Evans) 491–517.Google Scholar
  13. Hamparian, V.V., et al., 1987, A collaborative report: rhinoviruses-extension of the numbering system from 89 to 100, Virol. 159:191–192.CrossRefGoogle Scholar
  14. Hayden, F.G., Gwaltney, Jr., J.M., and Colonno, R J., 1988, Modification of experimental rhinovirus colds by receptor blockade, Antiviral Res. 9:233–247.PubMedCrossRefGoogle Scholar
  15. Jameson, B.A., et al., 1988, Location and chemical synthesis of a binding site for HIV-1 on the CD4 protein, Science 240:1335–1339.PubMedCrossRefGoogle Scholar
  16. Kaplan, G., Freistadt, M.S., and Racaniello, V.R., 1990, Neutralization of poliovirus by cell receptors expressed in insect cells, J. Virol. 64:4697–4702.PubMedGoogle Scholar
  17. Kim, S., et al., 1989, Crystal structure of human rhinovirus serotype 1A (HRV1A), J Mol Biol 210:91–111.PubMedCrossRefGoogle Scholar
  18. Landau, N.R., Warton, M., and Littman, D.R., 1988, The envelope glycoprotein of the human immunodeficiency virus binds to the immunoglobulin-like domain of CDA, Nature 334:159–162.PubMedCrossRefGoogle Scholar
  19. Lineberger, D.W., et al., 1990, Antibodies that block rhinovirus attachment map to domain 1 of the major group receptor, J. Virol. 64: 2582–2587.PubMedGoogle Scholar
  20. Makgoba, M.W., et al., 1988, Functional evidence that intercellular adhesion molecule-1 (ICAM-1) is a ligand for LFA-1 dependent adhesion in T cell-mediated cytotoxicity, Eur. J. Immunol. 18:637–640.PubMedCrossRefGoogle Scholar
  21. Mendelsohn, C.L., Wimmer, E., and Racaniello, V., 1989, Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily, Cell 56:855–865.PubMedCrossRefGoogle Scholar
  22. Mischak, H.C., et al., 1988, Detection of the human rhinovirus minor group receptor on renaturing Western blots, J. Gen. Virol. 69:2653–2656.PubMedCrossRefGoogle Scholar
  23. Mizutani, S., and Colonno, R.J., 1985, In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14, J. Virol 56:628–632.Google Scholar
  24. Naclerio, R.M., et al, 1988, Kinins are generated during experimental rhinovirus colds, J Infect. Dis. 157:133–142.Google Scholar
  25. Palmenberg, A.C., 1987, Picornaviral processing: some new ideas, J Cell. Biochem. 33:191–198.Google Scholar
  26. Peterson, A., and Seed, B. 1988, Genetic analysis of monoclonal antibody and HIV binding sites on the human lymphocyte antigen CD4, Cell 54:65–72.PubMedCrossRefGoogle Scholar
  27. Prevear, D.C., et al, 1989, Conformational change in the floor of the human rhinovirus canyon blocks adsorption to HeLa cell receptors, J Virol. 63:2002–2007.Google Scholar
  28. Rossmann, M.G., et al, 1985, Structure of a human common cold virus and functional relationship to other picornaviruses, Nature 317:145–153.PubMedCrossRefGoogle Scholar
  29. Sherry, B., et al, 1986, Use of monoclonal antibodies to identify four neutralization immunogens on a common cold Picornavirus, human rhinovirus 14, J Virol. 57:246–257.PubMedGoogle Scholar
  30. Simmons, D., Makgoba, M.W., and Seed, B., 1988, ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM, Nature 331:624–627.PubMedCrossRefGoogle Scholar
  31. Staunton, D.E., et al., 1988, Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families, Cell 52:925–933.PubMedCrossRefGoogle Scholar
  32. Staunton, D.E., et al., 1989, A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses, Cell 56:849–853.PubMedCrossRefGoogle Scholar
  33. Staunton, D.E., et al, 1990, The arrangement of the immunoglobulin-like domains of ICAM-1 and the binding sites for LFA-1 and rhinovirus, Cell, 61:243–254. PubMedCrossRefGoogle Scholar
  34. Tomassini, J.E., and Colonno, RJ., 1986, Isolation of a receptor protein involved in attachment of human rhinoviruses, J Virol. 58:290–295.PubMedGoogle Scholar
  35. Tomassini, J.E., Maxson, T.R., and Colonno, R.J., 1989b, Biochemical characterization of a glycoprotein required for rhinovirus attachment, J Biol Chem. 264:1656–1662.PubMedGoogle Scholar
  36. Tomassini, J.E., et al, 1989a, cDNA cloning reveals that the major group rhinovirus receptor on HeLa cells is intercellular adhesion molecule 1, Proc. Natl. Acad. Sci USA 86:4907–4911.PubMedCrossRefGoogle Scholar
  37. Uncapher, C.R., DeWitt, C.M., and Colonno, R.J., 1991, The major and minor group receptor families contain all but one human rhinovirus serotype, Virol, in press.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1992

Authors and Affiliations

  • Richard J. Colonno

There are no affiliations available

Personalised recommendations