Abstract
Classical membrane biophysics has been developed in carefully selected preparations that bear no direct relation to the mammalian central nervous system (CNS). This is easily understandable. Tissue derived from mammalian CNS is not only difficult to maintain and manipulate, but it also contains nerve cells with intricate dendritic processes that render the use of voltage-clamp techniques hazardous. Furthermore, their cellular surface is not easily accessible to experimental manipulations. All these sources of difficulty have been recognized for a long time. As a result, new technical developments such as the patch-clamp technique (Hamill et al., 1981) have received relatively few applications in the CNS, in contrast to the wealth of information gathered with this experimental approach in peripheral tissues. Much of the CNS work has concentrated on preparations such as the neuroendocrine cells of the pituitary gland, which can be isolated and studied in vitro relatively easily. These cells have a simple shape that is suited to voltage-clamp studies and they can be identified before recording by using the noninvasive hemolytic plaque assay (e.g. DeRiemer and Sakmann, 1986).
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Llano, I., Gähwiler, B.H., Marty, A. (1992). Voltage- and Transmitter-Gated Channels in Purkinje Cells from Organotypic Cerebellar Cultures. In: Llinás, R., Sotelo, C. (eds) The Cerebellum Revisited. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2840-0_9
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DOI: https://doi.org/10.1007/978-1-4612-2840-0_9
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