Preparation of DNA and RNA from Leaves: Expanded Blades and Separated Bundle Sheath and Mesophyll Cells
Part of the
Springer Lab Manuals
book series (SLM)
This preparation results in homogeneous intact mesophyll protoplasts suitable for nucleic acid isolation, assay of activities, or gene expression studies. The reader may wish to compare similar protocols used by Sheen (1990) for expression studies and by Kanai and Edwards (1973) for photosynthetic studies. The reader should be forewarned that the digestive enzymes used are expensive reagents.
KeywordsIsoamyl Alcohol Bundle Sheath Bundle Sheath Cell Guanidinium Thiocyanate Diethyldithiocarbamic Acid
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Springer-Verlag New York, Inc. 1994