Preparation of DNA and RNA from Leaves: Expanded Blades and Separated Bundle Sheath and Mesophyll Cells

  • Timothy Nelson
Part of the Springer Lab Manuals book series (SLM)


This preparation results in homogeneous intact mesophyll protoplasts suitable for nucleic acid isolation, assay of activities, or gene expression studies. The reader may wish to compare similar protocols used by Sheen (1990) for expression studies and by Kanai and Edwards (1973) for photosynthetic studies. The reader should be forewarned that the digestive enzymes used are expensive reagents.


Isoamyl Alcohol Bundle Sheath Bundle Sheath Cell Guanidinium Thiocyanate Diethyldithiocarbamic Acid 
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  2. Kanai R, Edwards GE (1973) Separation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies. Plant Physiol 51: 1133–1137PubMedCrossRefGoogle Scholar
  3. Langdale JA, Taylor WC, Nelson T (1991) Cell-specific accumulation of maize phosphoenolpyruvate carboxylase is correlated with demethylation at a specific site >3 kb upstream of the gene. Mol Gen Genet 225: 49–55PubMedCrossRefGoogle Scholar
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© Springer-Verlag New York, Inc. 1994

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  • Timothy Nelson

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