Analysis of Cytoplasmically Inherited Mutants
Although cytoplasmic mutants have been documented in higher plants, they have not been studied as well as in lower eukaryotes, such as yeast and Chlamydomonas. Very few potential chloroplast mutations have been described in maize, in contrast to several in Chlamydomonas and some in other higher plants. (See Börner and Sears 1986.) The only examples of cytoplasmically inherited, defective plastids in maize appear to result from the action of nuclear genes, such as iojap. (See Walbot and Coe 1979.) In plants homozygous for iojap, some plastids show a loss of chloroplast pigments and ribosomes. The ribosome-less plastids are then inherited maternally. Other nuclear gene mutations that affect photosynthetic function have been identified on the basis of a high chlorophyll fluorescence phenotype (hcf; Miles 1982). Over one hundred nuclear hcf mutations have now been identified in maize, and they are all inherited as typical, recessive Mendelian factors (Miles and Metz 1985).
KeywordsMaize Chlorophyll Agarose Hunt Alan
Unable to display preview. Download preview PDF.
- Allen JO, Emenhiser GK, Kermicle JL (1989) Miniature kernel and plant: interaction between teosinte cytoplasmic genomes and maize nuclear genomes. Maydica 34: 277–290Google Scholar
- Coe EH (1983) Maternally inherited abnormal plant types in maize. Maydica 28: 151–157Google Scholar
- Conde MF, Pring DR, Levings CS III (1979) Maternal inheritance of organelle DNA’s in Zea mays-Zea perennis reciprocal crosses. J Hered 70: 2–4Google Scholar
- Lauer M, Knudsen C, Newton KJ, Gabay-Laughnan S, Laughnan JR (1990) A partially deleted mitochondrial cytochrome oxidase 2 gene in the NCS6 abnormal growth mutant of maize. New Biolog 2: 179–186Google Scholar
- Miles D (1982) The use of mutations to probe photosynthesis in higher plants. In Edelman M, Hallick RB, Chua N-H (eds) Methods in Chloroplast Molecular Biology, Elsevier Science, New York, pp 76–107Google Scholar
- Miles D, Metz JG (1985) The role of nuclear genes of maize in chloroplast development. In Freeling M (ed) Plant Genetics, Alan R Liss, New York, pp 585–597Google Scholar
- Newton KJ, Coe EH Jr, Gabay-Laughnan S, Laughnan JR (1989) Abnormal growth phenotypes and mitochondrial mutations in maize. Maydica 34: 291–296Google Scholar