Advertisement

Analysis of Cytoplasmically Inherited Mutants

  • Kathleen J. Newton
Part of the Springer Lab Manuals book series (SLM)

Abstract

Although cytoplasmic mutants have been documented in higher plants, they have not been studied as well as in lower eukaryotes, such as yeast and Chlamydomonas. Very few potential chloroplast mutations have been described in maize, in contrast to several in Chlamydomonas and some in other higher plants. (See Börner and Sears 1986.) The only examples of cytoplasmically inherited, defective plastids in maize appear to result from the action of nuclear genes, such as iojap. (See Walbot and Coe 1979.) In plants homozygous for iojap, some plastids show a loss of chloroplast pigments and ribosomes. The ribosome-less plastids are then inherited maternally. Other nuclear gene mutations that affect photosynthetic function have been identified on the basis of a high chlorophyll fluorescence phenotype (hcf; Miles 1982). Over one hundred nuclear hcf mutations have now been identified in maize, and they are all inherited as typical, recessive Mendelian factors (Miles and Metz 1985).

Keywords

Cytoplasmic Male Sterility Chloroplast Pigment Mitochondrial Genotype Chloroplast Mutation Nuclear Gene Mutation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen JO, Emenhiser GK, Kermicle JL (1989) Miniature kernel and plant: interaction between teosinte cytoplasmic genomes and maize nuclear genomes. Maydica 34: 277–290Google Scholar
  2. Börner T, Sears BB (1986) Plastome mutants. Plant Molecular Biol Rep 4: 69–92CrossRefGoogle Scholar
  3. Coe EH (1983) Maternally inherited abnormal plant types in maize. Maydica 28: 151–157Google Scholar
  4. Conde MF, Pring DR, Levings CS III (1979) Maternal inheritance of organelle DNA’s in Zea mays-Zea perennis reciprocal crosses. J Hered 70: 2–4Google Scholar
  5. Hunt MD, Newton KJ (1991) The NCS3 mutation: genetic evidence for the expression of ribosomal protein genes rps3 and rpl16 in Zea mays mitochondria. EMBO J 10: 1045–1052PubMedGoogle Scholar
  6. Lauer M, Knudsen C, Newton KJ, Gabay-Laughnan S, Laughnan JR (1990) A partially deleted mitochondrial cytochrome oxidase 2 gene in the NCS6 abnormal growth mutant of maize. New Biolog 2: 179–186Google Scholar
  7. Laughnan JR, Gabay-Laughnan S (1983) Cytoplasmic male sterility in maize. Ann Rev Genet 17: 27–48PubMedCrossRefGoogle Scholar
  8. Miles D (1982) The use of mutations to probe photosynthesis in higher plants. In Edelman M, Hallick RB, Chua N-H (eds) Methods in Chloroplast Molecular Biology, Elsevier Science, New York, pp 76–107Google Scholar
  9. Miles D, Metz JG (1985) The role of nuclear genes of maize in chloroplast development. In Freeling M (ed) Plant Genetics, Alan R Liss, New York, pp 585–597Google Scholar
  10. Newton KJ, Coe EH Jr (1986) Mitochondrial DNA changes in abnormal growth (nonchromosomal stripe) mutants of maize. Proc Natl Acad Sci USA 83: 7363–7366PubMedCrossRefGoogle Scholar
  11. Newton KJ, Coe EH Jr, Gabay-Laughnan S, Laughnan JR (1989) Abnormal growth phenotypes and mitochondrial mutations in maize. Maydica 34: 291–296Google Scholar
  12. Newton KJ, Knudsen C, Gabay-Laughnan S, Laughnan JR (1990) An abnormal growth mutant in maize has a defective mitochondrial cytochrome oxidase gene. Plant Cell 2: 107–113PubMedGoogle Scholar
  13. Palmer JD, Herbon LA (1988) Plant mitochondrial DNA evolves rapidly in structure but slowly in sequence. J Mol Evol 28: 87–97PubMedCrossRefGoogle Scholar
  14. Pring DR, Levings CS III (1978) Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89: 121–136PubMedGoogle Scholar
  15. Roussell D, Thompson DL, Pallardy SG, Miles D, Newton KJ (1991) Chloroplast structure and function is altered in the NCS2 maize mitochondrial mutant. Plant Physiol 96: 232–238PubMedCrossRefGoogle Scholar
  16. Shumway LK, Bauman LF (1967) Nonchromosomal stripe of maize. Genetics 55: 33–38PubMedGoogle Scholar
  17. Walbot V, Coe EH Jr (1979) Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays. Proc Natl Acad Sci USA 76: 2760–2764PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Kathleen J. Newton

There are no affiliations available

Personalised recommendations