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Overview of Cloning Genes Using Transposon Tagging

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The Maize Handbook

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

Genes in which a transposable element has inserted can be cloned using hybridization probes specific for the adjacent transposable element. Three families of transposable elements, Ac/Ds, Spm(En), and Mu, have been used successfully to clone maize genes. Below I briefly summarize the strategies that have been utilized once an insertion mutation has been isolated and genetically characterized. See the appropriate section in Section III, Genetics Protocols, for a brief review of these transposable elements, to learn how to isolate mutations using the different element systems, and to confirm that the mutation is in fact the result of the desired element.

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Authors
  1. Vicki Chandler
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Editor information

Editors and Affiliations

  1. Department of Plant Biology, University of California, Berkeley, CA, 94720, USA

    Michael Freeling

  2. Department of Biological Sciences, Stanford University, Stanford, CA, 94305, USA

    Virginia Walbot

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© 1994 Springer-Verlag New York, Inc.

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Chandler, V. (1994). Overview of Cloning Genes Using Transposon Tagging. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_118

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  • DOI: https://doi.org/10.1007/978-1-4612-2694-9_118

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-0-387-94735-8

  • Online ISBN: 978-1-4612-2694-9

  • eBook Packages: Springer Book Archive

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