Overview of Cloning Genes Using Transposon Tagging
Genes in which a transposable element has inserted can be cloned using hybridization probes specific for the adjacent transposable element. Three families of transposable elements, Ac/Ds, Spm(En), and Mu, have been used successfully to clone maize genes. Below I briefly summarize the strategies that have been utilized once an insertion mutation has been isolated and genetically characterized. See the appropriate section in Section III, Genetics Protocols, for a brief review of these transposable elements, to learn how to isolate mutations using the different element systems, and to confirm that the mutation is in fact the result of the desired element.
KeywordsHigh Performance Liquid Chromatography Maize Codon Carotenoid Nash
Unable to display preview. Download preview PDF.
- Chandler VL, Hardeman KJ (1992) The Mu elements of Zea mays. In Scandalios J (ed) Advances in Genetics 30: 77–122, Academic Press, New YorkGoogle Scholar
- Dellaporta SL, Greenblatt I, Kermicle J, Hicks JB, Wessler SR (1988) Molecular cloning of the maize R-nj allele by transposon-tagging with Ac. In Gustafson JP, Appels R (eds) Chromosome Structure and Function: Impact of New Concepts, 18th Stadler Genetics Symposium, Plenum Press, New York, pp 263–282CrossRefGoogle Scholar
- Taylor LP, Chandler VL, Walbot V (1986) Insertion of 1.4 kb and 1.7 kb Mu elements into the bronzei gene of Zea mays L. Maydica XXXI: 31–45Google Scholar
- Viet B, Vollbrecht E, Mathern J, Hake S (1990) A tandem duplication causes the Kn1–0 allele of Knotted, a dominant morphological mutant of maize. Genetics 125: 623–631Google Scholar