Overview of Cloning Genes Using Transposon Tagging
Genes in which a transposable element has inserted can be cloned using hybridization probes specific for the adjacent transposable element. Three families of transposable elements, Ac/Ds, Spm(En), and Mu, have been used successfully to clone maize genes. Below I briefly summarize the strategies that have been utilized once an insertion mutation has been isolated and genetically characterized. See the appropriate section in Section III, Genetics Protocols, for a brief review of these transposable elements, to learn how to isolate mutations using the different element systems, and to confirm that the mutation is in fact the result of the desired element.
KeywordsTransposable Element Zein Gene Mutator Stock Methylation Sensitive Enzyme Maize Transposable Element
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