Advertisement

DNA Delivery into Maize Cell Cultures Using Silicon Carbide Fibers

  • H. F. Kaeppler
  • D. A. Somers
Part of the Springer Lab Manuals book series (SLM)

Abstract

Silicon carbide fibers can be used to deliver foreign DNA into maize tissue culture cells for transient expression studies and genetic transformation (Kaeppler et al. 1990, 1992). The fibers are easily handled and are very inexpensive. At its current level of development, frequencies of DNA delivery and stable transformation using the silicon carbide fiber method are somewhat lower than can be achieved with microprojectile bombardment (Klein et al. 1989; Spencer et al. 1990; Fromm et al. 1990; Gordon-Kamm et al. 1990). Further improvement of the silicon carbide fiber procedure will likely lead to increased DNA delivery frequencies. Materials and methods are given below for delivery of plasmid DNA into nonregenerable and regenerable maize cell cultures using silicon carbide fibers.

Keywords

Material Safety Data Sheet Material Safety Data Sheet Silicon Carbide Fiber Black Mexican Sweet Transient Expression Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Armstrong C, Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214CrossRefGoogle Scholar
  2. Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–839PubMedCrossRefGoogle Scholar
  3. Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O’Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Kreuger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2: 603–618PubMedGoogle Scholar
  4. Green CE (1977) Prospects for crop improvement in the field of cell culture. HortScience 12: 131–134Google Scholar
  5. Kaeppler HF, Gu W, Somers DA, Rines HW, Cockburn AF (1990) Silicon carbide fiber-mediated DNA delivery into plant cells. Plant Cell Rep 9: 415–418CrossRefGoogle Scholar
  6. Kaeppler HF, Somers DA, Rines HW, Cockburn AF (1992) Silicon carbide fiber-mediated stable transformation of plant cells. Theor Applied Genet, 84: 560–566Google Scholar
  7. Klein TM, Kornstein L, Sanford JC, Fromm ME (1989) Genetic transformation of maize cells by particle bombardment. Plant Physiol 91: 440–444PubMedCrossRefGoogle Scholar
  8. Spencer TM, Gordon-Kamm WJ, Daines RJ, Start WG, Lemaux PG (1990) Bialaphos selection of stable transformants from maize cell cultures. Theor Applied Genet 79: 625–631Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • H. F. Kaeppler
  • D. A. Somers

There are no affiliations available

Personalised recommendations