Advertisement

Retaining and Recovering Enzyme Activity During Degradation of TCE by Methanotrophs

  • A. V. Palumbo
  • J. M. Strong-Gunderson
  • S. Carroll
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB, volume 63-65)

Abstract

To determine if compounds added during trichloroethylene (TCE) degradation could reduce the loss of enzyme activity or increase enzyme recovery, different compounds serving as energy and carbon sources, pH buffers, or free radical scavengers were tested. Formate and formic acid (reducing power and a carbon source), as well as ascorbic acid and citric acid (free radical scavengers) were added during TCE degradation at a concentration of 2 mM. A saturated solution of calcium carbonate was also tested to address pH concerns. In the presence of formate and methane, only calcium carbonate and formic acid had a beneficial effect on enzyme recovery. The calcium carbonate and formic acid both reduced the loss of enzyme activity and resulted in the highest levels of enzyme activity after recovery.

Index Entries

TCE methanotrophs biodegradation enzyme activity formate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fliermans, C. B., Phelps, T. J., Ringleberg, D., Mikell, A. T., and White, D. C. (1988), Appl. Environ. Microbiol. 54, 1709–1714.Google Scholar
  2. 2.
    Henson, J. M., Yates, M. Y., and Cochran, J. W. (1989), J. Ind. Microbial. 4, 29–35.CrossRefGoogle Scholar
  3. 3.
    Jansen, D. B., Grobben, G., and Witholt, B. (1988), in Proceedings of the 4th European Congress on Biotechnology, Vol. 3, Neijssel, O. M., Van der Meer, R. R., and Luyben, K. C. A. M., eds., Elsevier Science Publishers, Amsterdam, pp. 515–518.Google Scholar
  4. 4.
    Little, C. D., Palumbo, A. V., Herbes, S. E., Lindstrom, M. E., Tyndall, R. L., and Gilmer, P. J. (1988), Appl. Environ. Microbiol. 54, 951–956.Google Scholar
  5. 5.
    Tsien, H-C., Brusseau, G. A., Hansong, R. S., and Wackett, L. P. (1989), Appl. Environ. Microbiol. 55, 3155–3161.Google Scholar
  6. 6.
    Zylstra, G. J., Wackett, L. P., and Gibson, D. T. (1989), Appl. Environ. Microbiol. 55, 3162–3166.Google Scholar
  7. 7.
    Wilson, J. T. and Wilson, B. H. (1985), Appl. Environ. Microbiol. 49 242–243.Google Scholar
  8. 8.
    Ensley, B. D. (1991), Ann. Rev. Microbiol. 45 283–299.CrossRefGoogle Scholar
  9. 9.
    Brusseau, G. A., Tsien, H-C., Hanson, R. S., and Wackett, L. P. (1990), Biodegradation 1 19–29.CrossRefGoogle Scholar
  10. 10.
    Eng, W., Palumbo, A. V., Sriharan, S., and Strandberg, G. W. (1991), Appl. Biochem. Biotechnol. 28/29 887–906.CrossRefGoogle Scholar
  11. 11.
    Alvarez-Cohen, L. and McCarty, P. L. (1991), Appl. Environ. Microbiol. 57, 228–235.Google Scholar
  12. 12.
    Henry, S. M. and Grbic-Galic, D. (1991), Appl. Environ, Microbial. 57 236–244.Google Scholar
  13. 13.
    Tschantz, M., Bowman, J., Donaldson, T. L., Bienkowski, P., Strong-Gunderson, J. M., Palumbo, A. V., Herbes, S. E., and Sayler, G. S. (1995), Environ. Sci. Technol. 29 2073–2082.CrossRefGoogle Scholar
  14. 14.
    Whittenbury, R., Phillips, K. C., and Wilkinson, J. F. (1970), J. Gen. Microbiol. 61 205–218.Google Scholar
  15. 15.
    Oldenhuis, R., Oedzes, J. Y., Van Der Warrde, J. J., and Janssen, D. B. (1991), Appt. Environ. Microbiol. 57, 7–14.Google Scholar
  16. 16.
    Uchiyama, H. (1995), J. Fermentation Bioengineering 79 608–613.CrossRefGoogle Scholar
  17. 17.
    Parvatiyar, M. G. (1995), Biotechnol. Biol. 50 57–64.CrossRefGoogle Scholar
  18. 18.
    Grbic-Galic, D. Henry, S. M., Godsy, E. M., Edwards, E., and Mayer, K. P. (1991), in R. Baker (ed). Organic Substances and Sediments in Water Vol. 3, Biological, Baker, R., ed., Lewis Publishers. Chelsea, MI, pp. 239–266.Google Scholar
  19. 19.
    Oldenhuis, R., Vink, J. M., Janssen, D. B., and Witholt, B. (1989), Appl. Environ. Microbiol. 55 2819–2826.Google Scholar

Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • A. V. Palumbo
    • 1
  • J. M. Strong-Gunderson
    • 1
  • S. Carroll
    • 1
  1. 1.Environmental Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA

Personalised recommendations