Retaining and Recovering Enzyme Activity During Degradation of TCE by Methanotrophs

  • A. V. Palumbo
  • J. M. Strong-Gunderson
  • S. Carroll
Part of the Applied Biochemistry and Biotechnology book series (ABAB, volume 63-65)


To determine if compounds added during trichloroethylene (TCE) degradation could reduce the loss of enzyme activity or increase enzyme recovery, different compounds serving as energy and carbon sources, pH buffers, or free radical scavengers were tested. Formate and formic acid (reducing power and a carbon source), as well as ascorbic acid and citric acid (free radical scavengers) were added during TCE degradation at a concentration of 2 mM. A saturated solution of calcium carbonate was also tested to address pH concerns. In the presence of formate and methane, only calcium carbonate and formic acid had a beneficial effect on enzyme recovery. The calcium carbonate and formic acid both reduced the loss of enzyme activity and resulted in the highest levels of enzyme activity after recovery.

Index Entries

TCE methanotrophs biodegradation enzyme activity formate 


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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • A. V. Palumbo
    • 1
  • J. M. Strong-Gunderson
    • 1
  • S. Carroll
    • 1
  1. 1.Environmental Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA

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