Abstract
Some important process properties of α-1,4-d-glucan phosphorylases isolated from the bacterium Corynebacterium callunae and potato tubers (Solanum tuberosum) were compared. Apart from minor differences in their stability and specificity (represented by the maximum degree of maltodextrin conversion) and a 10-fold higher affinity of the plant phosphorylase for maltodextrin (K M of 1.3 g/L at 300 mM of orthophosphate), the performances of both enzymes in a continuous ultrafiltration membrane reactor were almost identical. Product synthesis was carried out over a time course of 300–400 h in the presence or absence of auxiliary pullulanase (increasing the accessibility of the glucan substrate for phosphorolytic attack up to 15–20%). The effect of varied dilution rate and reaction temperature on the resulting productivities was quantitated, and a maximum operational temperature of 40°C was identified.
Index Entries
Author to whom all correspondence and reprint requests should be addressed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Newgard, C. B., Hwang, P. K., and Fletterick, R. J. (1989), Crit. Rev. Biochem. Mol. Biol. 24 69–99.
Tanizawa, K., Mori, H., Tagaya, M., and Fukui, T. (1994), in Molecular Aspects of Enzyme Catalysis, Fukui, T. and Soda, K., eds., VCH, Weinheim, Germany, pp. 107–126.
Guilbot, A. and Mercier, C. (1985), in The Polysaccharides, Aspinall, G.O., ed., Academic Press, Orlando, FL, pp. 210–283.
Palm, D., Klein, H., Schinzel, R., Buchner, M., and Helmreich, E. J. M. (1990), Biochemistry 29 1099–1107.
Hudson, J. W., Golding, G. B., and Crerar, M. M. (1993), J. Mol. Biol. 234 700–721.
Barford, D. and Johnson, L. N. (1989), Nature 340 609–616.
Hollo, J., Laszlo, E., and Juhasz, J. (1967), Plant a-Glucan Phosphorylase, Akademiai Kiado, Budapest, Hungary.
Kayane, S., Kawai, T., Sakata, M., Imamura, T., Tanigaki, M., and Kurosaki, T. (1989), EP 0 305 981 A2.
Weinhäusel, A., Nidetzky, B., Rohrbach, M., Blauensteiner, B., and Kulbe, K. D. (1994), Appl. Microbiol. Biotechnol. 41 510–516.
Weinhäusel, A., Nidetzky, B., Kysela, C., and Kulbe, K. D. (1995), Enzyme Microb. Technol., 17 130–135.
Nidetzky, B., Weinhäusel, A., Grießler, R., and Kulbe, K. D. (1995), J. Carbohydr. Chem. 14 1017–1028.
Vandamme, E., van Loo, J., Machtelinckx, L., and de Laporte, A. (1987), Adv. Appl. Microbiol. 32 163–201.
van Bekkum, H. (1991), in Carbohydrates as Organic Raw Materials. Lichtenthaler, F. W., ed., VCH, Weinheim, Germany, pp. 289–310.
Murao, S., Nagano, H., Ogura, S., and Nishino, T. (1985), Agric. Biol. Chem. 49 2113–2118.
Stabgier, P. and Thiem, J. (1991), in Enzymes in Carbohydrate Synthesis ACS Symp. Series 466, Bednarski, M. D. and Simon, E. S., eds., Washington, DC, pp. 63–78.
Prazeres, D. M. F. and Cabral, J. M. S. (1994), Enzyme Microb. Technol. 16 738–750.
Kula, M.-R. and Wandrey, C. (1987), Meth. Enzymol. 136 9–21.
Bradford, M. M. (1976), Anal. Biochem. 72 248–254.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc.
About this chapter
Cite this chapter
Nidetzky, B., Griessler, R., Weinhäusel, A., Haltrich, D., Kulbe, K.D. (1997). Reaction Engineering Aspects of α-1,4-d-Glucan Phosphorylase Catalysis. In: Davison, B.H., Wyman, C.E., Finkelstein, M. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology, vol 63-65. Humana Press. https://doi.org/10.1007/978-1-4612-2312-2_16
Download citation
DOI: https://doi.org/10.1007/978-1-4612-2312-2_16
Publisher Name: Humana Press
Print ISBN: 978-1-4612-7497-1
Online ISBN: 978-1-4612-2312-2
eBook Packages: Springer Book Archive