Abstract
The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli NZN111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. Induction of pMEA1, which encodes the native animoterminus, gave better overexpression of malic enzyme, approx 12-fold compared to uninduced cells. Under the appropriate culture conditions, expression of malic enzyme allowed the fermentative dissimilation of glucose by NZN111. The major fermentation product formed in induced cultures was succinic acid.
Author to whom all correspondence and reprint requests should be addressed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Jain, M. K., Datta, R., and Zeikus, J. G. (1989), in Bioprocess Engineering: The First Generation Ghose, T. K., ed., Norwood, Chichester, UK, pp. 366–389.
Clark, D. P. (1989), FEMS Microb. Rev. 63 223–234.
Blackwood, A. C., Neish, A. C., and Ledingham, G. A. (1956), J. Bacteriol. 72 497–499.
Millard, C. S., Chao, Y.-P., Liao, J. C., and Donnelly, M. I. (1996), Appl. Environ. Microbiol. 62 1808–1810.
Thauer, R. K., Jungermann, K., and Decker, K. (1977), Bacteriol. Rev. 41 100–180.
Murai, T., Tokushige, M., Nagai, J., and Katsuki, H. (1971), Biochem. Biophys. Res. Comm. 43 875–881.
Murai, T., Tokushige, M., Nagai, J., and Katsuki, H. (1972), J. Biochem. 71 1015–1028.
Landsperger, W. J. and Harris, B. G. (1976), J. Biol. Chem. 251 3599–3602.
Takeo, K., Murai, T., Nagai, J., and Katsuki, H. (1967), Biochem. Biophys. Res. Comm. 29 717.
Sanwal, B. D. (1970), J. Biol. Chem. 245 1212–1216.
Mallick, S., Harris, B. G., and Cook, P. F. (1991), J. Biol. Chem. 266 2732–2738.
Mat-Jan, F., Kiswar, A. Y., and Clark, D. P. (1989), J. Bacteriol. 171 342–348.
Boernke, W. E., Millard, C. S., Stevens, P. W., Kakar, S. N., Stevens, F. J., and Donnelly, M. I. (1995), Arch. Biochem. Biophys. 322 43–52.
Kulkarni, G., Cook, P. F., and Harris, B. G. (1993), Arch. Biochem. Biophys. 300 231–237.
Mahajan, S. K., Chu, C. C., Willis, D. K., Templin, A., and Clark, A. J. (1990), Genetics 125 261–273.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual,2nd ed., 2nd ed., Cold Spring Harbor Press, Cold Spring Harbor, NY.
Balch, W. and Wolfe, R. S. (1976), Appl. Environ. Microbiol. 32 781–791.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc.
About this chapter
Cite this chapter
Stols, L., Kulkarni, G., Harris, B.G., Donnelly, M.I. (1997). Expression of Ascaris suum Malic Enzyme in a Mutant Escherichia coli Allows Production of Succinic Acid from Glucose. In: Davison, B.H., Wyman, C.E., Finkelstein, M. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology, vol 63-65. Humana Press. https://doi.org/10.1007/978-1-4612-2312-2_15
Download citation
DOI: https://doi.org/10.1007/978-1-4612-2312-2_15
Publisher Name: Humana Press
Print ISBN: 978-1-4612-7497-1
Online ISBN: 978-1-4612-2312-2
eBook Packages: Springer Book Archive