Tracking the Target Protein

  • Ian M. Rosenberg

Abstract

Protein analysis and purification is aimed at elucidating the structure and function of proteins. In order to analyze a protein, you must have a reliable detection system that unambiguously enables you to follow the target protein. This is especially true when the target molecule is in crude or even semipurified form. Often, analytical results will lay the groundwork for a successful purification scheme.

Keywords

DMSO Tyrosine Iodine Glycine Cysteine 

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References

  1. Argetsinger LS, Campbell GS, Yang X, Witthuhn BA, Silvennoinen O, Ihle JN, Carter-Su C (1993): Identification of JAK2 as a growth hormone receptor-associated tyrosine kinase. Cell 74: 237–244CrossRefPubMedGoogle Scholar
  2. Bolton AE, Hunter WM (1973): The labeling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J 153: 272–278Google Scholar
  3. Cochet C, Kashles O, Chambaz EM, Borrello I, King CR, Schlessinger J (1988): Demonstration of epidermal growth factor-induced receptor dimerization in living cells using a chemical covalent cross-linking agent. J Biol Chem 263: 3290–3295PubMedGoogle Scholar
  4. Cole SR, Ashman LK, Ev PL (1987): Biotinylation: an alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates. Mol Immunol 24: 699–705CrossRefPubMedGoogle Scholar
  5. Crawford AW, Beckerle MC (1991): Purification and characterization of zyxin, an 82,000-dalton component of adherens junctions. J Biol Chem 266: 5847–5853PubMedGoogle Scholar
  6. Degen E, Williams DB (1991): Participation of a novel 88-kD protein in the biogenesis of murine class I histocompatibility molecules. J Cell Biol 112: 1099–1115CrossRefPubMedGoogle Scholar
  7. Fields S, Song OK (1989): A novel genetic system to detect protein—protein interactions. Nature 340: 245–246CrossRefPubMedGoogle Scholar
  8. Flaumenhaft R, Abe M, Sato Y, Miyazono K, Harpel J, Heldin C-H, Rifkin DB (1993): Role of the latent TGF-ß binding protein in the activation of latent TGF-ß by co-cultures of endothelial smooth muscle cells. J Cell Biol 120: 995–1002CrossRefPubMedGoogle Scholar
  9. Fraker PJ, Speck JC (1978): Protein and cell membrane iodinations with a sparingly soluble chloramide,1,3,4,6-Tetrachloro-3α,6α-diphem®lglycouril. Biochem Biophys Res Comm 80: 849–857CrossRefPubMedGoogle Scholar
  10. Greenlund AC, Schreiber RD, Goeddel DV, Pennica D (1993): Interferon-gamma induces receptor dimerization in solution and on cells. J Biol Chem 268: 18103–18110PubMedGoogle Scholar
  11. Harlow E, Lane D (1988): Antibodies, A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor LaboratoryGoogle Scholar
  12. Haustein D (1975): Effective radioiodination by lactoperoxidase and solubilization of cell-surface proteins of cultured murine T-lymphoma cells. J Immunol Methods 7: 25–38CrossRefPubMedGoogle Scholar
  13. Hunziker W, Harter C, Matter K, Mellman I (1991): Basolateral sorting in MDCK cells requires a distinct cytoplasmic domain determinant. Cell 66: 907–920CrossRefPubMedGoogle Scholar
  14. Kessler SW (1975): Rapid isolation of antigens from cells with a Staphlococcal protein A-antibody absorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 115: 1617–1624PubMedGoogle Scholar
  15. Kessler SW (1981): Use of protein A-bearing Staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods Enzymol 73: 442–459CrossRefPubMedGoogle Scholar
  16. Köhler G, Milstein C (1975): Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497CrossRefPubMedGoogle Scholar
  17. Lisanti MP, Sargiacomo M, Graeve L, Saltiel AR, Rodriguez-Boulan E (1988): Polarized apical distribution of glycosyl-phosphatidylinositol anchored proteins in a renal epithelial cell line. Proc Natl Acad Sci USA 85: 9557–9561Google Scholar
  18. Maciver SK, Zot HG, Pollard TD (1991): Characterization of actin filament severing actophorin from Acanthamoeba Castellanii. J Cell Biol 115: 1611–1620CrossRefPubMedGoogle Scholar
  19. McConahey PJ, Dixon FJ (1980): Radioiodination of proteins by the use of the chloramine-T method. Methods Enzymol 70: 210–213CrossRefPubMedGoogle Scholar
  20. Musil LS, Goodenough DA (1993): Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER. Cell 74: 1065–1077CrossRefPubMedGoogle Scholar
  21. On W-J, Cameron PH, Thomas DY, Bergeron JJM (1993): Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364: 771–776CrossRefGoogle Scholar
  22. Phizicky E, Bastiaens PIH, Zhu H, Snyder M, Fields S (2003): Protein analysis on a proteomic scale. Nature 422: 208–215CrossRefPubMedGoogle Scholar
  23. Rosenberg I, Cherayil B, Isselbacher KJ, Pillai S (1991): Mac-2 binding glycoproteins. J Biol Chem 266: 18731–18736PubMedGoogle Scholar
  24. Sabarth N, Lamer S, Zimny-Arndt U, Jungblut PR, Meyer TF, Bumann D (2002): Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification, and two-dimensional gel elwectrophoresis. J Biol Chem 277: 27896–27902CrossRefPubMedGoogle Scholar
  25. Sali A, Glaeser R, Earnest T, Baumeister W (2003): From words to literature in structural proteomics. Nature 422: 2126–225CrossRefGoogle Scholar
  26. Schächter V (2002): Bioinformatics of large scale protein interaction networks. BioTechniques Suppl 32: S16–S27Google Scholar
  27. Shin BK, Wang H, Yim AM, Le Naour F, Brichory F, Jong JH, Zhao R, Purays E, Tra J, Michael CW, Misek DE, Hanash SM (2003): Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function. J Biol Chem 278: 7607–7616CrossRefPubMedGoogle Scholar
  28. Sober HA (1968): CRC Handbook of Biochemistry. Cleveland, OH: CRC PressGoogle Scholar
  29. Wiese C, Galande S (2001) Elimination of reducing agent facilitates quantitative detection of p53 antigen. BioTechniques 30:960–963PubMedGoogle Scholar
  30. Wong SS (1991): Chemistry of Protein Conjugation and Cross-Linking. Boca Raton, FL: CRC PressGoogle Scholar
  31. Wouters FS, Verveer PJ, Bastiaens PIH (2001): Imaging biochemistry inside cells. Trends Cell Biol 11: 203–211CrossRefPubMedGoogle Scholar
  32. Wu R, Plopper CG, Cheng P-W (1991): Mucin-like glycoprotein secreted by cultured hamster tracheal epithelial cells. Biochem J 277: 713–718CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Ian M. Rosenberg
    • 1
  1. 1.Jackson 715, G.I. UnitMassachusetts General HospitalBostonUSA

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