Protein analysis and purification is aimed at elucidating the structure and function of proteins. In order to analyze a protein, you must have a reliable detection system that unambiguously enables you to follow the target protein. This is especially true when the target molecule is in crude or even semipurified form. Often, analytical results will lay the groundwork for a successful purification scheme.
KeywordsSodium Dodecyl Sulfate Target Protein Fluorescence Resonance Energy Transfer Sucrose Gradient Glycine Solution
Unable to display preview. Download preview PDF.
- Bolton AE, Hunter WM (1973): The labeling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J 153: 272–278Google Scholar
- Harlow E, Lane D (1988): Antibodies, A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor LaboratoryGoogle Scholar
- Lisanti MP, Sargiacomo M, Graeve L, Saltiel AR, Rodriguez-Boulan E (1988): Polarized apical distribution of glycosyl-phosphatidylinositol anchored proteins in a renal epithelial cell line. Proc Natl Acad Sci USA 85: 9557–9561Google Scholar
- Schächter V (2002): Bioinformatics of large scale protein interaction networks. BioTechniques Suppl 32: S16–S27Google Scholar
- Sober HA (1968): CRC Handbook of Biochemistry. Cleveland, OH: CRC PressGoogle Scholar
- Wong SS (1991): Chemistry of Protein Conjugation and Cross-Linking. Boca Raton, FL: CRC PressGoogle Scholar