An Overview of This Manual

Abstract

This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a protein purification or protein analysis project. The protocols may be performed in the order laid out or may be used independently. The aim of the book is to assist a wide range of researchers, from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the prominently numbered steps, one can work through any protocol, whether it’s a new technique or a task that has been previously performed for which a quick review or updated methodology is needed.