Pretreatment of Sugarcane Bagasse Hemicellulose Hydrolysate for Xylitol Production by Candida guilliermondii

  • Lourdes A. Alves
  • Maria G. A. Felipe
  • João B. Almeida E. Silva
  • Silvio S. Silva
  • Arnaldo M. R. Prata
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

In order to remove or reduce the concentrations of toxic substances present in the sugarcane bagasse hemicellulose hydrolysate for xylose-to-xylitol bioconversion, the hydrolysate was pretreated by changing the initial pH level through the combination of different bases and acids with or without the subsequent addition of activated charcoal. Attention was given to the influence of the fermentation time as well.

The experiments were based on multivariate statistical concepts, with the application of fractional factorial design techniques to identify the important variables in the process. Subsequently, the levels of these variables were quantified by the response surface methodology, which permitted the establishment of the best pretreatment procedure with a xylose-to-xylitol bioconversion efficiency of 86.2%. This procedure consisted in increasing the pH of the hydrolysate from 0.5 to 7.0 with CaO and reducing it to 5.5 with H3PO4. Next, the hydrolysate was treated with activated charcoal (2.4%). The highest xylitol yield (0.79 g/g) corresponded to a productivity of 0.47 g/L/h.

Index Entries

Sugarcane bagasse hydrolysate pretreatment xylitol Candida guilliermondii 

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References

  1. 1.
    Felipe, M. G. A., Vitolo, M., and Mancilha, I. M. (1996A), Acta Biotechnologica 16, 73–79.CrossRefGoogle Scholar
  2. 2.
    Felipe, M. G. A., Alves, L. A., Silva, S. S., Roberto, I. C, Mancilha, I. M., and Almeida e Silva, J. B., (1996B) Bioresource Technol. 56, 281–283.CrossRefGoogle Scholar
  3. 3.
    Melaja, A. J. and Hämäläinen, L. (1997), Process for Making Xylitol. US Patent no. 4.008.285. 1975, 15 fev.Google Scholar
  4. 4.
    Domingues, J. M., Cheng, S. G., and Tsao, G. T. (1996), Applied Biochem. Biotechnol. 57/58, 49–56.CrossRefGoogle Scholar
  5. 5.
    Meyrial, V., Delgenes, J. P. Molletta, R., and Navarro, J. M. (1991), Biotechnol. Lett. 13, 281–286.CrossRefGoogle Scholar
  6. 6.
    Nolleau, V., Preziosi-Belloy, L., Delgenes, J. P., and Delgenes, J. M. (1993), Curr. Microbiol. 27, 191–197.CrossRefGoogle Scholar
  7. 7.
    Felipe, M. G. A., Vitolo, Mancilha, I. M., and Silva, S. S. (1997), J. Indust. Microbiol. 18, 251–254.CrossRefGoogle Scholar
  8. 8.
    Roberto, I. C, Felipe, M. G. A., Lacis, L. S., Silva, S. S., and Mancilha, L M. (1991), Bioresource Technol. 36, 271–275.CrossRefGoogle Scholar
  9. 9.
    Barbosa, M. F. S., Medeiros, M. B., Mancilha, I. M., Schneider, H., and Lee, H. (1988), J. Indust. Microbiol. 3, 241–251.CrossRefGoogle Scholar
  10. 10.
    Felipe, M. G. A., Vieira, D. C, Vitolo. M., Silva, S. S., Roberto, I. C., Mancilha, I. M., and Rosa, S. A. M. (1995), J. Basic Microbiol. 35, 171–177.CrossRefGoogle Scholar
  11. 11.
    Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978), Statistics for Experimenters: An Introduction to Design, Data Analysis and Model Building. Wiley, New York.Google Scholar
  12. 12.
    Barros Neto, B., Scarminio, I. S., and Bruns, R. E. (1995), Planejamento e Otimizaçao de Experimentes, 1 ed., Editora da UNICAMP, Campinas.Google Scholar
  13. 13.
    Chen, L. F. and Gong, C. S. (1985), J. Food Sci. 50, 226–228.CrossRefGoogle Scholar
  14. 14.
    Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Roberto, I. C., Silva, S. S., and Rosa, S. A. M. (1993), Arquivos de Biologia e Technologia, 36, 103–114.Google Scholar
  15. 15.
    Roberto I. C., Mancilha, I. M., Souza, C. M. A., Felipe, M. G. A., Sato, S., and Castro, H. F. (1994), Biotechnol. Lett. 16, 1211–1216.CrossRefGoogle Scholar
  16. 16.
    van Zyl, C., Prior, B. A., and Du Preez, J. C. (1988), Appl. Biochem. Biotechnol. 17, 357–369.CrossRefGoogle Scholar
  17. 17.
    Parajó, J. C., Domingues, H., and Domingues, J. M. (1996B) Bioresource Technol. 57, 179–185.CrossRefGoogle Scholar
  18. 18.
    Parajó, J. C, Domingues, H., and Domingues, J. M. (1996A), Biotechnol. Lett. 18, 593–598.CrossRefGoogle Scholar
  19. 19.
    Chung, I. S., and Lee, Y. Y. (1985), Biotechnol Bioengineer. 27, 308–315.CrossRefGoogle Scholar
  20. 20.
    Wang, R. C., Kuo, C. C, and Shyu, C. C. (1997), J. Chem. Tech. Biotechnol. 68, 187–194.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Lourdes A. Alves
    • 1
  • Maria G. A. Felipe
    • 1
  • João B. Almeida E. Silva
    • 1
  • Silvio S. Silva
    • 1
  • Arnaldo M. R. Prata
    • 1
  1. 1.Departamento de BiotecnologiaFAENQUILLorenaBrazil

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