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Ethanol Production from AFEX-Treated Forages and Agricultural Residues

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Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

Lignocellulosic materials derived from forages, namely timothy grass, alfalfa, reed canary grass, and agricultural residues, such as corn stalks and barley straw, were pretreated using ammonia fiber explosion (AFEX) process. The pretreated materials were directly saccharified by cellulolytic enzymes. Sixty to 80% of theoretical yield of sugars were obtained from the pretreated biomasses. Subsequent ethanolic fermentation of the hydrolysates by Pachysolen tannophilus ATCC 32691 resulted in 40-60% of theoretical yield after 24 h, based on the sugars present in the hydrolysates. The uptake of sugars was not complete, indicating a possible inhibitory effect on P. tannophilus during the fermentation of these substrates.

Author to whom correspondence and reprint requests should be addressed. E-mail: ginette.turcotte@aln.ulaval.ca

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References

  1. Alvo, P., Savoie, P., Tremblay, D., Emond, J. P., and Turcotte, G. (1996), Bioresource Technol. 56, 61–68.

    Article  CAS  Google Scholar 

  2. Stone, J. E., Scallan, A. M., Danefer, E., and Ahlgren, E. (1969), Adv. Chem. Serv. 95, 219–223.

    Article  CAS  Google Scholar 

  3. Fan, L. T., Lee, Y. H., and Gharpuray, M. M. (1982), Adv. Biochem. Eng. Biotechnol. 23, 157–187.

    CAS  Google Scholar 

  4. Fan, L. T., Lee, Y. R., and Beardmore, D. H. (1980), Biotechnol. Bioeng. 22, 177–199.

    Article  CAS  Google Scholar 

  5. Sinitsyn, A. P., Gusakov, A. V., and Vlasenko, E. Y. (1991), Appl. Biochem. Biotechnol. 30, 43–59.

    Article  CAS  Google Scholar 

  6. Himmel, M., Tucker, M., Baker, J., Rivard C, Oh, K., and Grohmann, K. (1985), Biotechnol. Bioeng. Symp. 15, 39–58.

    Google Scholar 

  7. Millett, M. A., Baker, A. J., and Satter, L. D. (1976), Biotechnol. Bioeng. Symp. 6, 125–153.

    CAS  Google Scholar 

  8. Brownell, H. H. and Saddler, J. N. (1984), Biotechnol. Bioeng. Symp. 14, 55–68.

    CAS  Google Scholar 

  9. Chua, M. G. S., and Wayman, M. (1979), Can. J. Chem. 57, 1141–1146.

    Article  CAS  Google Scholar 

  10. Shultz, T. P., Biermann, C. J., and Mc Ginnis, G. D. (1983), Ind. Eng. Chem. Prod. Res. Dev. 22, 344–348.

    Article  Google Scholar 

  11. Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985), J. Wood Chem. Tech. 5/3, 405–425.

    Article  Google Scholar 

  12. Belkacemi, K., Turcotte, G., Savoie, P., and Chornet, E. (1997), Ind. Eng. Chem. Res. 36, 4572–4580.

    Article  CAS  Google Scholar 

  13. Iyer, P. V., Wu, Z. W., Kim, S. B., and Yoon, Y. L. (1996), Appl. Biochem. Biotechnol. 57/58, 121–132.

    Article  CAS  Google Scholar 

  14. Yoon, H. H., Wu, Z. W., and Lee, Y., Y. (1994), Appl. Biochem. Biotechnol. 51/52, 5–19.

    Article  Google Scholar 

  15. Kim, S. B. and Lee, Y. Y. (1996), Appl Biochem. Biotechnol. 57/58, 147–156.

    Article  CAS  Google Scholar 

  16. Dale, B. E. and Moreira, M. J. (1982), Biotechnol. Bioeng. Symp. 12, 31–43.

    CAS  Google Scholar 

  17. Bothast, R. J., Dien, B. S., Iten, L. B., Hespell, R. B., and Lawton, J. W. (1996), in Liquid Fuels and Industrial Products from Renewable Resources, Proceedings of the Liquid Fuel Conference, Nashville, TN, ASAE St-Joseph, pp. 241–252.

    Google Scholar 

  18. Dale, B. E. and de 1a Rosa, L. (1992), in Liquid Fuels from Renewable Resources, Proceedings of an Alternative Energy Conference, Nashville, TN, ASAE St-Joseph, pp. 162–170.

    Google Scholar 

  19. Dale, B. E., Leong, C. K., Pham, T. K., Esquivel, V. M., Rios, I., and Latimer, V. M. (1994), in Liquid Fuels, Lubricants and Additives from Biomass, Proceedings of an Alternative Energy Conference, Nashville, TN, ASAE St-Joseph, pp. 104–111.

    Google Scholar 

  20. De 1a Rosa, L., Reshamwala, S., Latimer, V. M., Shawky, B. T., Dale, B. E., and Stuwart, E. D. (1994), Appl. Biochem. Biotechnol. 45/46, 483–497.

    Article  Google Scholar 

  21. Holzapple, M. T., Jun, J. H., Ashok, G., Patibandla, S. L., and Dale, B. E. (1991), Appl. Biochem. Biotechnol. 28/29, 59–74.

    Article  Google Scholar 

  22. Holzapple, M. T., Ripley, E. P., and Nikolaou, M. (1994), Biotechnol Bioeng. 44, 1122–1131.

    Article  Google Scholar 

  23. Moniruzzaman, M., Dien, B. S., Ferrer, B., Hespell, R. B., Dale, B. E., Ingram, L. O., and Bothast, R. J. (1996), Biotechnol. Lett. 18, 985–990.

    Article  CAS  Google Scholar 

  24. Miller, G. L. (1959), Anal. Chem. 31, 426–428.

    Article  CAS  Google Scholar 

  25. Belkacemi, K., Turcotte, G., de Halleux, D., and Savoie, P. (1996), in Liquid Fuels and Industrial Products from Renewable Resources, Proceedings of the Liquid Fuel Conference, Nashville, TN, ASAE St-Joseph, pp 232–240.

    Google Scholar 

  26. Chiquette, J. (1997), in Proceedings of the 1997 Ethanol Research and Development Workshop, Ottawa, ON, pp 111–114.

    Google Scholar 

  27. Beck, M. J. (1986), Biotechnol Bioeng. Symp. 17, 615–627.

    Google Scholar 

  28. Coughlan, M. P. (1985), Biotechnol Gen. Eng. Rev. 3, 39–109.

    Article  CAS  Google Scholar 

  29. Ramos, L. P., Breuil, C., and Saddler, J. N. (1993), Enzyme Microbiol. Technol. 15, 19–25.

    Article  CAS  Google Scholar 

  30. Saddler, J. N. (1986), Microbiol. Sci. 3, 84–87.

    CAS  Google Scholar 

  31. Wood, T. M. (1989), in Enzymes Systems for Lignocellulose Degradation, Coughlan, M. P., ed., Elsevier, London, pp. 17–35.

    Google Scholar 

  32. Christov, L., and Prior, B. (1993), Enzyme Microbiol. Technol. 15, 460–475.

    Article  CAS  Google Scholar 

  33. Penner, M. H. and Liaw, E. T. (1994), in Enzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., ACS Symposium Series 566, pp. 363–371.

    Google Scholar 

  34. Panchal, C. J., Bast, L., Russell, I., and Stewart, G. G. (1988), Can. J. Microbiol. 34, 1316–1320.

    Article  CAS  Google Scholar 

  35. Dubus, D., Methner, H., Shulze, D., and Dellweg, H. (1983), Eur. J Appl. Microbiol. Biotechnol. 17, 287–291.

    Article  Google Scholar 

  36. Jeffries, T. W. (1990), in Yeast Biotechnology and Biocatalysis, Verachtert, H. and De Mot, R., eds. Marcel Dekker, Louvain, Belgium, pp. 349–393.

    Google Scholar 

  37. Dien, B. S., Kurtzman, C. P., Sana, B. C, and Bothast, R. J. (1996), Appl. Biochem. Biotechnol. 57/58, 233–242.

    Article  Google Scholar 

  38. Ligthelm, M. E., Prior, B. A., and du Preez, J. C. (1988), Appl. Microbiol. Biotechnol. 28, 293–296.

    CAS  Google Scholar 

  39. Wilkie, K. C. B. (1979), in Advances Carbohydrate Chemistry and Biochemistry, Vol. 36 Tipson, R. S., and Horton, D., eds., Academic, New York pp. 215–264.

    Google Scholar 

  40. Alvo, P. and Belkacemi, K. (1997), Bioresource Technol. in press.

    Google Scholar 

  41. Fan, L. T., Gharpuray, M. M., and Lee, Y. H. (1987), in Cellulose Hydrolysis, Springer-Verlag, New York, pp. 5–120.

    Google Scholar 

  42. Poutanen, K. and Puls, J. (1989), in Biogenesis and Biodegradation of Plant Cell Wall Polymers, Lewis, G. and Paice, M., eds., American Chemical Society, Washington D. C, pp. 456–467.

    Google Scholar 

  43. Doran, J. B. and Ingram, L. O. (1993), Biotechnol. Prog. 9, 533–538.

    Article  CAS  Google Scholar 

  44. Ohta, K., Beall, D. S., Mejia, J. P., Shanmugam, K. T., and Ingram, L. O. (1991), Appl. Environ. Microbiol 57, 893–900.

    CAS  Google Scholar 

  45. Takahashi, D. F., Carvalhal, M. L., and Alterthum, F. (1994), Biotechnol Lett. 16, 747–750.

    Article  CAS  Google Scholar 

  46. York, S. W. and Ingram, L. O. (1996), Biotechnol. Lett. 18, 683–688.

    Article  CAS  Google Scholar 

  47. Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995), Science 267, 240–243.

    Article  CAS  Google Scholar 

  48. Schneider, H., Wang, P. Y., Chan, Y. K., and Maleska, R. (1981), Biotechnol. Lett. 3, 89–92.

    Article  CAS  Google Scholar 

  49. Slininger, P. J., Bothast, R. J., Van Cauwenberge, J. E., and Kurtzman, C. P. (1982), Biotechnol. Bioeng. 24, 371–384.

    Article  CAS  Google Scholar 

  50. Slininger, P. J., Bolen, P. L., and Kurtzman, C. P. (1987), Enzyme Microbiol. Technol. 9, 5–15.

    Article  CAS  Google Scholar 

  51. Delgenes, J. P., Moletta, R., and Navarro, J. M. (1986), Biotechnol. Lett. 8, 897–900.

    Article  CAS  Google Scholar 

  52. Woods, M. A. and Millis, N. F. (1985), Biotechnol Lett. 7, 679–682.

    Article  CAS  Google Scholar 

  53. Du Preez, J. C. and Prior, B. A. (1985), Biotechnol. Lett. 7, 241–246.

    Article  Google Scholar 

  54. Olsson, L., and Hahn-Hägerdal, B. (1996), Enzyme Microbiol. Technol. 18, 312–331.

    Article  CAS  Google Scholar 

  55. Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995), Science 267, 240.

    Article  CAS  Google Scholar 

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Belkacemi, K., Turcotte, G., de Halleux, D., Savoie, P. (1998). Ethanol Production from AFEX-Treated Forages and Agricultural Residues. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_42

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  • DOI: https://doi.org/10.1007/978-1-4612-1814-2_42

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7295-3

  • Online ISBN: 978-1-4612-1814-2

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