Abstract
Alkalophylic bacilli that produce cyclodextringlycosyltransferase (CGTase) were isolated from Brazilian soil, with a scheme of two plating steps. In the first step, the bacterial isolate forms a halo in the cultivation medium that contains 7-cyclodextrin (CD) complexing dyes. The CGTase of an isolate was purified 157-fold by biospecific affinity chromatography, with β-CD showing a mol wt of 77,580 Daltons. It produces a 7- to β-CD ratio of 0.156 and a small amount of α-CD, using maltodextrin 10% as substrate, at 50¼, pH 8.0 and 22 h reaction time, reaching 21.4% conversion of the substrate to cyclodextrins. In the second screening step, the isolates chosen give larger halos with β-CD complexing dyes, and smaller halos with β-CD complexing dyes, leading to a 30% improvement in γ-CD selectivity, although at lower total yield for cyclodextrins (11.5%).
Author to whom all correspondence and reprint requests should be addressed, e-mail: flavio@cybertelecom.com.br
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Matioli, G., Zanin, G.M., De Moraes, F.F., Guimaraes, M.F. (1998). Production and Purification of CGTase of Alkalophylic Bacillus Isolated from Brazilian Soil. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_26
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DOI: https://doi.org/10.1007/978-1-4612-1814-2_26
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