Abstract
During a screening for the enzyme pyranose 2-oxidase (P2O) which has a great potential as a biocatalyst for carbohydrate transformations, Trametes multicolor was identified as a promising, not-yet-described producer of this particular enzyme activity. Furthermore, it was found in this screening that the enzyme frequently occurs inbasidiomycetes. Intracellular P2O was produced in a growth-associated manner by T. multicolor during growth on various substrates, including mono-, oligo-, and polysaccharides. Highest levels of this enzyme activity were formed when lactose or whey were used as substrates. Peptones from casein and other casein hydrolysates were found to be the most favorable nitrogen sources for the formation of P2O. By applying an appropriate feeding strategy for the substrate lactose, which ensured an elevated concentration of the carbon source during the entire cultivation, levels of P2O activity obtained in laboratory fermentations, as well as the productivity of these bioprocess experiments, could be enhanced more than 2.5-fold.
Author to whom all correspondence and reprint requests should be addressed.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Janssen, F. W. and Ruelius, H. W. (1975), Methods Enzymol. 41, 170–173.
Volc, J., Sedmera, P., and Musílek, V. (1978), Folia Microbiol. 23, 292–298.
Machida, Y., and Nakanishi, T. (1984), Agric. Biol. Chem. 48, 2463–2470.
Volc, J., and Eriksson, K.-E. (1988), Methods Enzymol 161, 316–322.
Izumi, Y., Furuya, Y., and Yamada, H. (1990), Agric. Biol Chem. 54, 1393–1399.
Danneel, H.-J., Rössner, E., Zeeck, A., and Giffhorn, F. (1993), Eur. J. Biochem. 214, 795–802.
Daniel, G., Volc, J., and Kubátová, E. (1994), Appl. Environ. Microbiol. 60, 2524–2532.
Daniel, G. (1994), FEMS Microbiol Rev. 13, 199–233.
Baute, M.-A., Baute, R., Deffieux, G., and Filleau, M.-J. (1977), Phytochemistry 16, 1895–1897.
Baute, M.-A. and Baute, R. (1984), Phytochemistry 23, 271–274.
Volc, J., Kubátová, E., Sedmera, P., Daniel, G., and Gabriel, J. (1991), Arch. Microbiol. 156, 297–301.
Gabriel, J., Volc, J., Sedmera, P., Daniel, G., and Kubátová, E. (1993), Arch. Microbiol. 160, 27–34.
Röper, H. (1991), in Carbohydrates as Organic Raw Materials, Lichtenthaler, F. W., ed., VCH, Weinheim, Germany, pp. 267–288.
Crueger, A. and Crueger, W. (1990), in Microbial Enzymes and Biotechnology, Fogarty, W. M. and Kelly, C. T., eds., Elsevier Applied Science, London, pp. 177–226.
Huwig, A., Danneel, H.-J., and Giffhorn, F. (1994), J. Biotechnol. 32, 309–315.
Neidleman, S. L., Amon, W. F., and Geigert, J. (1981), U.S. Patent 4246347.
Liu, T.-N. E., Wolf, B., Geigert, J., Neidleman, S. L., Chin, J. D., and Hirano, D. S. (1983), Carbohydr. Res. 113, 151–157.
Geigert, J., Neidleman, S. L., and Hirano, D. S. (1983), Carbohydr. Res. 113, 159–162.
Danneel, H.-J., Ullrich, M., and Giffhorn, F. (1992), Enzyme Microb. Technol. 14, 898–903.
Petroski, R. J., Peczynska-Czoch, W., and Rosazza, J. P. (1980), Appl. Environ. Microbiol. 40, 1003–1006.
Bradford, M. M. (1976), Anal Biochem. 72, 248–254.
Furuya, Y., Yamada, H., and Izumi, Y. (1993), J. Ferment. Bioeng. 76, 532–534.
Gancedo, J. M., Gancedo, C., and Asensio, C. (1967), Arch. Biochem. Biophys. 119, 588–590.
Volc, J., Denisova, N. P., Nerud, F., and Musílek, V. (1985), Folia Microbiol. 30, 141–147.
Eriksson, K.-E., Pettersson, B., Volc, J., and Musilek, V. (1986), Appl. Microbiol Biotechnol. 23, 257–262.
Ruelius, H. W., Kerwin, R. ML, and Janssen, F. W. (1968), Biochim. Biophys. Acta 167, 493–500.
Thomas, C. R. (1990), in Chemical Engineering Problems in Biotechnology, Winkler, M. A., ed., Elsevier Applied Science, London, pp. 23–93.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Leitner, C., Haltrich, D., Nidetzky, B., Kulbe, K.D., Prillinger, H. (1998). Production of a Novel Pyranose 2-Oxidase by Basidiomycete Trametes multicolor. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_23
Download citation
DOI: https://doi.org/10.1007/978-1-4612-1814-2_23
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-7295-3
Online ISBN: 978-1-4612-1814-2
eBook Packages: Springer Book Archive