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Hematopoietic Stem Cell Processing and Transplantation

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Red Cell Transfusion

Part of the book series: Contemporary Hematology ((CH))

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Abstract

Transplantation of hemapoietic stem cell (HSCs) is an accepted treatment for hematological diseases such as severe aplastic anemia and most leukemias. In This setting, the HSC are used tom replace the diseased or damaged bone marrow (BM) (13).More recently, HSC have been used to restore BM function following treatment with high dose chemotheraphy and/or radiation in patients with lymphomas and some solid tumors (4,5). With the use of recombinant colony stimulating factors (CSFs) with or without the addition of cytotoxic drugs, it is possible to mobilize large numbers of stem and progenitor cells into the peripheral blood for collection by apheresis. These peripheral blood stem cells (PBSCs) can be used alone or in combination with BM to regenerate hematopoiesis. Advances in cell separation have made it possible to refine BM and PBSC grafts so that specific cell populations may be isolated and infused (6,8). Incubation with certain cytokines and pharmacologic agents can also induce in vitro proliferation of some cell populations while inhibiting others. These manipulated cells can then be used for adoptive immunotherapy(9,10), as vehicles for gene therapy(11), and perhaps even induce tolerance of an organ transplant from the same donor.

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Authors
  1. Patricia L. Kotula
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  2. Ellen M. Areman
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  3. Ronald A. Sacher
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Editors and Affiliations

  1. New York Blood Center, NY, USA

    Marion E. Reid PhD

  2. American Red Cross Blood Services, Philadelphia, PA, USA

    Sandra J. Nance MS, MT(ASCP)SBB

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Kotula, P.L., Areman, E.M., Sacher, R.A. (1998). Hematopoietic Stem Cell Processing and Transplantation. In: Reid, M.E., Nance, S.J. (eds) Red Cell Transfusion. Contemporary Hematology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1798-5_8

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  • DOI: https://doi.org/10.1007/978-1-4612-1798-5_8

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