From Genotype to Phenotype
- 208 Downloads
The idea that rates of degradation influence the time course of approach to new, steady-state concentrations applies to mRNAs and enzymes, but the two-compartment model is incomplete. It does not account for the processes by which transcription is activated, which frequently involve the regulation of DNA-binding proteins by ligands or by covalent modification. Also it does not include the processes involved in mRNA processing and transport, or the impact of the completed protein on cellular metabolism. To be useful to the molecular biologist, a simulation tool should be able to describe any intermediate that could potentially affect the outcome of an experiment, and this requires information about each rate of formation, transfer, or elimination (Hargrove, 1993). In the sequential reactions of gene expression, the processing or transfer of each intermediate not only affects the yield, but also introduces a delay that is proportional to the half-time of each step. Many molecular biologists need to make predictions concerning the effects of altered transcription, nuclear mRNA metabolism, and translational or posttransla-tional controls.
KeywordsTranslational Control Molecular Biologist Nucleocytoplasmic Transport Altered Transcription Hyperbolic Curf
Unable to display preview. Download preview PDF.
- Fersht, A. Enzyme structure and mechanism. New York: W.H. Freeman and Co., 1985.Google Scholar
- Koshland, D.E., Jr. “Switches, thresholds, and ultrasensitivity.” TIBS 12 (1987): 225–229.Google Scholar
- Levitzki, Alexander. In: Receptors: A Quantitative Approach. Menlo Park, Cal.: Benjamin/Cummings Publishing Co., 1984.Google Scholar
- Taylor, P., and P.A. Insel. “Molecular basis of pharmacologic selectivity.” In: Principles of Drug Action, The Basis of Pharmacology, Pratt, W.B., and P. Taylor, eds. New York: Churchill Livingstone, 1990.Google Scholar