A Preliminary Information About Continuous Fermentation Using Cell Recycling for Improving Microbial Xylitol Production Rates

Scientific Note
  • Silvio S. Silva
  • Adolfo Q. Chanto
  • Michele Vitolo
  • Maria G. A. Felipe
  • Ismael M. Mancilha
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Xylitol is a sugar-alcohol with important technological properties, such as anticariogenicity, low caloric value, and negative dissolution heat. It can be used successfully in food formulations and pharmaceutical industries. Its production is therefore in great demand. Biotechnological xylitol production has several economic advantages in comparison with the conventional process based on the chemical reduction of xylose. The efficiency and the productivity of this fermentation chiefly depends on the microorganism and the process conditions employed. In this article a simple continuous culture with cell recycling was evaluated to enhance the capability of Candida guilliermondii FTI 20037 to produce xylitol. The fermentation was initiated batchwise by directly inoculating the grown seed culture in a 2-L bench-scale fermentor. Continuous feeding was begun at a dilution rate (D) of 0.060Jh after the xylose concentration had completely consumed and the cell concentration was about 4.0 gJL. At a dilution rate of 0.060Jh the xylitol concentration was about 15 gJL and increased by about 35%, whereas the dilution rate decreased by about 58%. Furthermore, the volumetric productivity, Qp, markedly depended on the dilution rate, diminishing by about 37% as D was changed from 0.060 to 0.025Jh. These preliminary results show us that the continuous fermentation with cell recycling is a good way to study the xylitol production by xylose-fermenting yeasts

Index Entries

Xylitol yeast continuous fermentation recycling 

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Silvio S. Silva
    • 1
  • Adolfo Q. Chanto
    • 2
  • Michele Vitolo
    • 3
  • Maria G. A. Felipe
    • 1
  • Ismael M. Mancilha
    • 1
  1. 1.Department of BiotechnologyFaculty of Chemical Engineering of Lorena, FAENQUIL/DEBIQLorena-São PauloBrazil
  2. 2.Biotechnological Development Center (CDB)Joinville, SCBrazil
  3. 3.Department of Biochemical and Pharmaceutical TechnologyUniversity of São PauloSPBrazil

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