Conversion of Industrial Food Wastes by Alcaligenes Latus into Polyhydroxyalkanoates

  • Peter H. Yu
  • Hong Chua
  • Ai-Ling Huang
  • Kwok-Ping Ho
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


Broader usage of biodegradable plastics in packaging and disposable products as a solution to environmental problems would heavily depend on further reduction of costs and the discovery of novel biodegradable plastics with improved properties. As the first step in our pursuit of eventual usage of industrial food wastewater as nutrients for microorganisms to synthesise environmental-friendly bioplastics, we investigated the usage of soya wastes from a soya milk dairy, and malt wastes from a beer brewery plant as the carbon sources for the production of polyhydroxyalkanoates (PHA) by selected strain of microorganism. Bench experiments showed that Alcali-genes latus DSM 1124 used the nutrients from malt and soya wastes to biosynthesise PHAs. The final dried cell mass and specific polymer production of A. latus DSM 1124 were 32g/L and 70% polymer/cells (g/g), 18.42 g/L and 32.57% polymer/cell (g/g), and 28 g/L and 36% polymer/cells (g/g), from malt waste, soya waste, and from sucrose, respectively. These results suggest that many types of food wastes might be used as the carbon source for the production of PHA.

Index Entries

Polyhydroxyalkanoate (PHA) polyhydroxybutyrate (PHB) malt soya waste Alcaligenes Alcaligenes latus 


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  1. 1.
    Byrom, D. (1994), in Plastics from microbes: microbial synthesis of polymers and polymer precursors, Mobley, D. P., ed., Hanser, Munich, pp. 5–11.Google Scholar
  2. 2.
    Yu, P. and Giuliany, B. (1989), Frontiers 9(11), 4–7.Google Scholar
  3. 3.
    Lee, S. Y. (1995), Biotechnol. Bioeng. 49, 1–14.CrossRefGoogle Scholar
  4. 4.
    Helmut, B., Gross, R. A., Lenz, R. W., and Fuller, R. C. (1990), Adv. Biochem. Eng. /BioTechnol. 41, 78–91.Google Scholar
  5. 5.
    Linko, S., Vaheri, H., and Seppala, J. (1993), Enzyme Microbial. Technol. 15(5), 401–406.CrossRefGoogle Scholar
  6. 6.
    Lee, S. Y. (1996), Trends BioTechnol. 14, 431–438.CrossRefGoogle Scholar
  7. 7.
    Yamane, T., Fukunagga, M., and Lee, Y. W. (1996), Biotechnol Bioeng. 50(2), 197–202.CrossRefGoogle Scholar
  8. 8.
    Kim B. S. and Chang, H. S. (1995), Biotechnol. Techniques 9(5), 311–314.CrossRefGoogle Scholar
  9. 9.
    Alderte, J. E., Karl, D. W., and Park, C. H. (1993), Biotechnol. Prog. 9(5), 520–525.CrossRefGoogle Scholar
  10. 10.
    Shah N. N., Hanna, M. L., and Taylor, R. T. (1996), Biotechnol. Bioeng. 49(2), 161–171.CrossRefGoogle Scholar
  11. 11.
    Son, H. and Lee, S. (1996), Biotechnology Lett. 18, 1217–1222.CrossRefGoogle Scholar
  12. 12.
    Yu, P. H., Chua, H., and Ho, L. Y. (1996), Proceedings of the Asia-Pacific Conference on Sustainable Energy and Environmental Technology, Singapore, pp. 623–630.Google Scholar
  13. 13.
    Yu, P. H., Chua, H., Huang, A. L., Lo, W., and Chen, J. C. (1998), Conversion of food industrial wastes into bioplastics. Appl. Biochem. Biotechnol. Google Scholar
  14. 14.
    Gomez, J. G. C, Rodrigues, M. F. A., Alii, R. C. P., Torres, B. B., Bueno Netto, C. L, Oliverira, M. S., and da Silva, L. F. (1996), Appl. Microbiol. BioTechnol. 45, 785–791.CrossRefGoogle Scholar
  15. 15.
    Hahn S. K., Chang, Y. K., Kim, B. S., and Chang, H. N. (1994), Biotechnol. Bioeng. 44(2), 256–261.CrossRefGoogle Scholar
  16. 16.
    Roh, K. S., Yeom, S. H., and Yoo, Y. J. (1995), Biotechnol. Techniques. 9(10), 709–771.CrossRefGoogle Scholar
  17. 17.
    Greenberg, A. E., Clesceri, L. S., and Eton, A. D. (1992), TKN analysis (5310C). Standard Methods for the Examination of Water and Wastewater, 18th ed., Apha, Washington, DC.Google Scholar
  18. 18.
    Jan, S., Roblot, C, Goethals, G., Courtois, J., Courtois, B., Saucedo, J. E. N., Seguin, J. P., and Barbotin, J. N. (1995), Anal. Biochem. 225, 258–263.CrossRefGoogle Scholar
  19. 19.
    Jan S., Roblot, C, Courtois, J., Courtois, B., Barbotin, J. N., and Seguin, J. P. (1996), Enzyme Microbial. Technol. 18, 195–201.CrossRefGoogle Scholar
  20. 20.
    Hu, W. F., Chua, H., and Yu, P. H. F. (1997), Biotechnol. Lett. 19, 695–698.CrossRefGoogle Scholar
  21. 21.
    Chua, H., Yu, P. H. F., and Ho, L. Y. (1997), Appl. Biochem. BioTechnol. 63, 627–635.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Peter H. Yu
    • 1
  • Hong Chua
    • 2
  • Ai-Ling Huang
    • 1
  • Kwok-Ping Ho
    • 1
  1. 1.Union Laboratory of Asymmetric Synthesis and Department of Applied Biology and Chemical TechnologyChina
  2. 2.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHong KongChina

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