Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

  • Lyudmila Vasilyeva
  • Masato Miyake
  • Chikashi Nakamura
  • Eiji Nakada
  • Anatoly Tsygankov
  • Yasuo Asada
  • Jun Miyake
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as well as the SD sequence and the start codon from pufB. Translational puf:luc fusion was constructed to analyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated more than 700-fold under anaerobic light conditions. When Rb. sphaeroides (pPluc-2) strain was grown photoheterotrophically in batch culture, the luciferase expression was observed during 7 h. The expression time could be greatly extended using continuous cultivation in a photobioreactor.

Index Entries

Rhodobacter sphaeroides expression vector puf promoter luciferase continuous culture 


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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Lyudmila Vasilyeva
    • 1
    • 2
    • 3
    • 5
  • Masato Miyake
    • 1
  • Chikashi Nakamura
    • 4
  • Eiji Nakada
    • 3
  • Anatoly Tsygankov
    • 5
  • Yasuo Asada
    • 1
  • Jun Miyake
  1. 1.National Institute of Bioscience and Human TechnologyTsukuba, IbarakiJapan
  2. 2.Research Institute of Innovative Technology for the EarthJapan
  3. 3.Fuji Electric Corporate R&D, Ltd.KanagawaJapan
  4. 4.National Institute of Advanced Interdisciplinary ResearchIbarakiJapan
  5. 5.Institute of Basic Biological ProblemsRussian Academy of SciencePushchinoRussia

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