Abstract
An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as well as the SD sequence and the start codon from pufB. Translational puf:luc fusion was constructed to analyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated more than 700-fold under anaerobic light conditions. When Rb. sphaeroides (pPluc-2) strain was grown photoheterotrophically in batch culture, the luciferase expression was observed during 7 h. The expression time could be greatly extended using continuous cultivation in a photobioreactor.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Donohue, T. J. and Kaplan, S. (1991), Methods Enzymol. 204, 459–485.
Williams, J. C. and Taguchi, A. K. W. (1995), in Anoxygenic Photosynthetic Bacteria, Blankenship, R. E., Madigan, M. T., and Bauer, C. E., eds., Kluwer, Dordrecht, The Netherlands, pp. 1029–1065.
Farchaus, J. W. and Oesterhelt, D. (1989), EMBO J. 8, 47–54.
Narro, M. L., Adams, C. W., and Cohen, S. N. (1990), J. Bacteriol. 172, 4549–4554.
Hunter, C. N., McGlynn, P., Ashby, M. K., Burgess, J. G., and Olsen, J. D. (1991), Mol. Microbiol. 5, 2649–2661.
Bolliwar, D. W., Suzuki, J. Y., Beatty, J. T., Dobrowolski, J. M., and Bauer, C. (1994), J. Mol. Biol. 237, 622–640.
Johnson, J. A., Wong, W. K. R., and Beatty, J. T. (1986), J. Baderiol. 167, 604–610.
Bauer, C, Young, D. A., and Marrs, B. L. (1988), J. Biol. Chem. 263, 4820–4827.
Klug, G. (1993), Arch. Microbiol. 159, 397–404.
Duport, C, Meyer, C, Naud, I., and Jouanneau, Y. (1994), Gene 145, 103–108.
Miyake, J., Mao, X.-Y., and Kawamura, S. (1984), J. Ferment. Technol. 62, 531–535.
Suzuki, T., Tsygankov, A., Miyake, J., Tokiwa, Y., and Asada, Y. (1995), Biotechnol. Lett. 17, 395–400.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
DeHoff, B. S., Lee, J. K., Donohue, T. J., Gumport, R. I., and Kaplan, S. (1988), J. Bacteriol. 170, 4681–4692.
Mak, Y. M. and Ho, K. K. (1991), Nucleic Acids Res. 20, 4101–4102.
Simon, R., Priefer, U., and Puhler, A. (1983), Bio/Technology 1, 784–791.
Keen, N. T., Tamaki, S., Kobayashi, D., and Trollinger, D. (1988), Gene 70, 191–197.
Platt, T. (1986), Annu. Rev. Biochem. 55, 339–372.
Bauer, C. E. (1995), in Anoxygenic Photosynthetic Bacteria, Blankenship, R. E., Madigan, M. T., and Bauer, C. E., eds., Kluwer, Dordrecht, The Netherlands, pp. 1221–1234.
Tsygankov, A. A. and Laurinavichene, T. V. (1996), Biotechnol. Bioengin. 51, 605–612.
Lee, J. K., Kiley, P. J., and Kaplan, S. (1989), J. Bacteriol. 171, 3391–3405.
Pollock, D., Bauer, C. E., and Scolnik, P. A. (1988), Gene 65, 269–275.
Kondo, T. and Ishiura, M. (1994), J. Bacteriol. 176, 1881–1885.
Yanisch-Perron, C, Vieira, J., and Messing, J. (1985), Gene 33, 103–109.
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994), Science 263, 802–805.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Vasilyeva, L. et al. (1999). Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides . In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_31
Download citation
DOI: https://doi.org/10.1007/978-1-4612-1604-9_31
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-7214-4
Online ISBN: 978-1-4612-1604-9
eBook Packages: Springer Book Archive