Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

  • Lyudmila Vasilyeva
  • Masato Miyake
  • Chikashi Nakamura
  • Eiji Nakada
  • Anatoly Tsygankov
  • Yasuo Asada
  • Jun Miyake
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as well as the SD sequence and the start codon from pufB. Translational puf:luc fusion was constructed to analyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated more than 700-fold under anaerobic light conditions. When Rb. sphaeroides (pPluc-2) strain was grown photoheterotrophically in batch culture, the luciferase expression was observed during 7 h. The expression time could be greatly extended using continuous cultivation in a photobioreactor.

Index Entries

Rhodobacter sphaeroides expression vector puf promoter luciferase continuous culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Donohue, T. J. and Kaplan, S. (1991), Methods Enzymol. 204, 459–485.CrossRefGoogle Scholar
  2. 2.
    Williams, J. C. and Taguchi, A. K. W. (1995), in Anoxygenic Photosynthetic Bacteria, Blankenship, R. E., Madigan, M. T., and Bauer, C. E., eds., Kluwer, Dordrecht, The Netherlands, pp. 1029–1065.Google Scholar
  3. 3.
    Farchaus, J. W. and Oesterhelt, D. (1989), EMBO J. 8, 47–54.Google Scholar
  4. 4.
    Narro, M. L., Adams, C. W., and Cohen, S. N. (1990), J. Bacteriol. 172, 4549–4554.Google Scholar
  5. 5.
    Hunter, C. N., McGlynn, P., Ashby, M. K., Burgess, J. G., and Olsen, J. D. (1991), Mol. Microbiol. 5, 2649–2661.CrossRefGoogle Scholar
  6. 6.
    Bolliwar, D. W., Suzuki, J. Y., Beatty, J. T., Dobrowolski, J. M., and Bauer, C. (1994), J. Mol. Biol. 237, 622–640.CrossRefGoogle Scholar
  7. 7.
    Johnson, J. A., Wong, W. K. R., and Beatty, J. T. (1986), J. Baderiol. 167, 604–610.Google Scholar
  8. 8.
    Bauer, C, Young, D. A., and Marrs, B. L. (1988), J. Biol. Chem. 263, 4820–4827.Google Scholar
  9. 9.
    Klug, G. (1993), Arch. Microbiol. 159, 397–404.CrossRefGoogle Scholar
  10. 10.
    Duport, C, Meyer, C, Naud, I., and Jouanneau, Y. (1994), Gene 145, 103–108.CrossRefGoogle Scholar
  11. 11.
    Miyake, J., Mao, X.-Y., and Kawamura, S. (1984), J. Ferment. Technol. 62, 531–535.Google Scholar
  12. 12.
    Suzuki, T., Tsygankov, A., Miyake, J., Tokiwa, Y., and Asada, Y. (1995), Biotechnol. Lett. 17, 395–400.CrossRefGoogle Scholar
  13. 13.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  14. 14.
    DeHoff, B. S., Lee, J. K., Donohue, T. J., Gumport, R. I., and Kaplan, S. (1988), J. Bacteriol. 170, 4681–4692.Google Scholar
  15. 15.
    Mak, Y. M. and Ho, K. K. (1991), Nucleic Acids Res. 20, 4101–4102.CrossRefGoogle Scholar
  16. 16.
    Simon, R., Priefer, U., and Puhler, A. (1983), Bio/Technology 1, 784–791.CrossRefGoogle Scholar
  17. 17.
    Keen, N. T., Tamaki, S., Kobayashi, D., and Trollinger, D. (1988), Gene 70, 191–197.CrossRefGoogle Scholar
  18. 18.
    Platt, T. (1986), Annu. Rev. Biochem. 55, 339–372.CrossRefGoogle Scholar
  19. 19.
    Bauer, C. E. (1995), in Anoxygenic Photosynthetic Bacteria, Blankenship, R. E., Madigan, M. T., and Bauer, C. E., eds., Kluwer, Dordrecht, The Netherlands, pp. 1221–1234.Google Scholar
  20. 20.
    Tsygankov, A. A. and Laurinavichene, T. V. (1996), Biotechnol. Bioengin. 51, 605–612.CrossRefGoogle Scholar
  21. 21.
    Lee, J. K., Kiley, P. J., and Kaplan, S. (1989), J. Bacteriol. 171, 3391–3405.Google Scholar
  22. 22.
    Pollock, D., Bauer, C. E., and Scolnik, P. A. (1988), Gene 65, 269–275.CrossRefGoogle Scholar
  23. 23.
    Kondo, T. and Ishiura, M. (1994), J. Bacteriol. 176, 1881–1885.Google Scholar
  24. 24.
    Yanisch-Perron, C, Vieira, J., and Messing, J. (1985), Gene 33, 103–109.CrossRefGoogle Scholar
  25. 25.
    Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994), Science 263, 802–805.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Lyudmila Vasilyeva
    • 1
    • 2
    • 3
    • 5
  • Masato Miyake
    • 1
  • Chikashi Nakamura
    • 4
  • Eiji Nakada
    • 3
  • Anatoly Tsygankov
    • 5
  • Yasuo Asada
    • 1
  • Jun Miyake
  1. 1.National Institute of Bioscience and Human TechnologyTsukuba, IbarakiJapan
  2. 2.Research Institute of Innovative Technology for the EarthJapan
  3. 3.Fuji Electric Corporate R&D, Ltd.KanagawaJapan
  4. 4.National Institute of Advanced Interdisciplinary ResearchIbarakiJapan
  5. 5.Institute of Basic Biological ProblemsRussian Academy of SciencePushchinoRussia

Personalised recommendations