2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis
The glucose analog 2-deoxyglucose (2-DOG) has been used to obtain mutants derepressed for pentose metabolism. Some researchers have used 2-DOG alone whereas others have used it in the presence of a glucose-repressible carbon source. We examined both methods and screened mutant strains for improved use of xylose in the presence of glucose. Pichia stipitis mutants selected for growth on D-xylose in the presence of 2-DOG used xylose from a 1:1 glucose:xylose mixture more rapidly than did their parents. One of these mutants, FPL-DX26, completely consumed xylose in the presence of glucose and produced 33 g/L ethanol in 45 h from 80 g/L of this sugar mixture. Mutants selected for growth on 2-DOG alone did not show significant improvement. Selection for growth on D-xylose in the presence of 2-DOG has been useful in developing parental strains for further genetic manipulation.
Index EntriesPichia stipitis mutation selection 2-deoxyglucose (2-DOG) fermentation ethanol glucose repression
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- 2.Delgenes, J. P., Moletta, R., and Navarro, J. M. (1988), Appl. Microbiol. Biotechnol. 29, 155–161.Google Scholar
- 10.McCracken, L. D. and Gong, C. S. (1983), Adv. Biochem. Bioeng. 27, 33–85.Google Scholar
- 13.D’Amore, T. and Dowhanick, T. M. (1994), J. Am. Soc. Brew. Chem. 52, 12–15.Google Scholar
- 14.Entian, K. D. and Fröhlich, K.-U. (1984), J. Bacteriol. 158, 29–35.Google Scholar
- 16.Jones, R. M., Russell, I., and Stuart, G. G. (1986), J. Am. Soc. Brew. Chem. 44, 161–166.Google Scholar
- 20.Jeffries, T. W. and Livingston, P. L. (1992), U. S. Patent No. 5, 126, 266.Google Scholar
- 23.Jeffries, T. W. (1982), Biotechnol. Bioeng. Symp. 12, 103–110.Google Scholar