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2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis

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Twentieth Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

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Abstract

The glucose analog 2-deoxyglucose (2-DOG) has been used to obtain mutants derepressed for pentose metabolism. Some researchers have used 2-DOG alone whereas others have used it in the presence of a glucose-repressible carbon source. We examined both methods and screened mutant strains for improved use of xylose in the presence of glucose. Pichia stipitis mutants selected for growth on D-xylose in the presence of 2-DOG used xylose from a 1:1 glucose:xylose mixture more rapidly than did their parents. One of these mutants, FPL-DX26, completely consumed xylose in the presence of glucose and produced 33 g/L ethanol in 45 h from 80 g/L of this sugar mixture. Mutants selected for growth on 2-DOG alone did not show significant improvement. Selection for growth on D-xylose in the presence of 2-DOG has been useful in developing parental strains for further genetic manipulation.

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Author information

Authors and Affiliations

  1. Institute of Microbial Biochemical Technology, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, Wl, 53705, USA

    Hassan K. Sreenath & Thomas W. Jeffries

  2. Department of Bacteriology, University of Wisconsin-Madison, Madison, Wl, USA

    Thomas W. Jeffries

Authors
  1. Hassan K. Sreenath
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  2. Thomas W. Jeffries
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Corresponding author

Correspondence to Thomas W. Jeffries .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison

  2. National Renewable Energy Laboratory, USA

    Mark Finkelstein

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© 1999 Springer Science+Business Media New York

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Sreenath, H.K., Jeffries, T.W. (1999). 2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis . In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_21

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  • DOI: https://doi.org/10.1007/978-1-4612-1604-9_21

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7214-4

  • Online ISBN: 978-1-4612-1604-9

  • eBook Packages: Springer Book Archive

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