Skip to main content

Fertilizing Capability of Frozen-Thawed Immotile Sperm

  • Chapter
Male Sterility and Motility Disorders

Abstract

The introduction of intracytoplasmic sperm injection (ICSI) for severe male cases has made it possible to treat patients with azoospermia, in whom sperm is achieved from the epididymis and the testis (1–3). In some of the treated male patients, especially those with non-obstructive azoospermia, mainly immotile spermatozoa are available. Because it is assumed that the fertilizing potential of testicular sperm is lower (4) and motility is an important predictive factor for successful fertilization (5) it should be questioned whether ICSI perfomed with immotile sperm is justified.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Schoysman R, Vanderzwalmen P, Nijs M, et al. Pregnancy after fertilisation with human testicular spermatozoa. Lancet 1993;342:1237.

    Article  PubMed  CAS  Google Scholar 

  2. Devroey P, Liu J, Nagy Z, Tournaye H, Silber SJ, Van Steirteghem AC. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil Steril 1994;62:639–41.

    PubMed  CAS  Google Scholar 

  3. Devroey P, Liu J, Nagy Z, et al. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod 1995;10:101–4.

    Article  Google Scholar 

  4. Nagy Z, Liu J, Janssenswillen C, Silber S, Devroey P, Van Steirteghem AC. Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil Steril 1995;63:808–15.

    PubMed  CAS  Google Scholar 

  5. Nagy Z, Liu J, Joris H, et al. The results of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 1995;20:1123–29.

    Google Scholar 

  6. Verheyen G, Nagy Z, Joris H, De Croo I, Tournaye H, Van Steirteghem AC. Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes. Fertil Steril 1997;67:74–80.

    Article  PubMed  CAS  Google Scholar 

  7. Ron-El R, Strassburger D, Friedler S, et al. Extended sperm preparation: an alternative to testicular sperm extraction in non-obstructive azoospermia. Hum Reprod 1997;12:1222–26.

    Article  PubMed  CAS  Google Scholar 

  8. Friedler S, Raziel A, Strassburger D, Soffer Y, Komarovsky D, Ron-El R. Testicular sperm retrieval by percutaneous fine needle sperm aspiration compared with testicular sperm extraction by open biopsy in men with non-obstructive azoospermia. Hum Reprod 1997;12:1488–93.

    Article  PubMed  CAS  Google Scholar 

  9. Cohen J, Feiten P, Zeilmaker GH. In vitro fertilizing capacity of fresh and cryopreserved human spermatozoa: a comparative study of freezing and thawing procedures. Fertil Steril 1981;36:356–62.

    PubMed  CAS  Google Scholar 

  10. Edirisinghe RW, Junk SM, Matson LP, Yovitch JL. Changes in motility patterns during in-vitro culture of fresh and frozen/thawed testicular and epididymal spermatozoa: implications for planning treatment by intracytoplasmic sperm injection. Hum Reprod 1996;11:2474–76.

    Article  PubMed  CAS  Google Scholar 

  11. Kahraman S, Isik AZ, Vicdan K, Ozgur S, Ozgun OD. A healthy birth after intracytoplasmic sperm injection by using immotile testicular spermatozoa in a case with totally immotile ejaculated spermatozoa before and after Percoll gradients. Hum Reprod 1997;12:292–93.

    Article  PubMed  CAS  Google Scholar 

  12. Romero J, Remohi J, Minguez Y, Rubio C, Pellicer A, Gil-Salom M. Fertilization after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Fertil Steril 1996;65:877–79.

    PubMed  CAS  Google Scholar 

  13. Gil-Salom M, Romero J, Minguez Y, et al. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod 1996;11:1309–13.

    Article  PubMed  CAS  Google Scholar 

  14. Hovatta O, Foudila T, Siegberg R, Johansson K, von Smitten K, Reima I. Pregnancy resulting from intracytoplasmic injection of spermatozoa from frozen-thawed testicular biopsy specimen. Hum Reprod 1996;11:2472–73.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer Science+Business Media New York

About this chapter

Cite this chapter

Ron-El, R. et al. (1999). Fertilizing Capability of Frozen-Thawed Immotile Sperm. In: Hamamah, S., Olivennes, F., Mieusset, R., Frydman, R. (eds) Male Sterility and Motility Disorders. Serono Symposia USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-1522-6_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-1522-6_10

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7177-2

  • Online ISBN: 978-1-4612-1522-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics