A Novel Magnetite-Immobilized Cell Process for Heavy Metal Removal from Industrial Effluent

  • Wang Lei
  • H. Chua
  • W. H. Lo
  • P. H. F. Yu
  • Y. G. Zhao
  • P. K. Wong
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


The sorption and desorption of copper (II) (Cu[II]) ions from the wastewater by magnetite-immobilized cells of Pseudomonas putida 5-x with acidic pretreatment were studied. Pretreating cells with 0.6 N HC1 was found to enhance greatly the adsorption capacity of biomass up to 85.6 mg/g and had no significant effect on the loss of P. putida 5-x cells during biosorbent pretreatment. The biosorption capacity to Cu2+ of magnetite-immobilized cells of P. putida 5-x harvested during various growth phases was also investigated. The experimental results illustrated that the adsorption capacity to Cu2+ of P. Putida 5-x cultured in sulfate-limiting medium reached maximum during the late stationary growth phase or early death phase, and reached minimum during the log growth phase. The mechanism of copper sequestering by this type of biomass was studied via transmission electron microscopy. A degradation of the peptidoglycan layer of the cell wall was observed in the acidic pretreatment, but no further degradation appeared after the adsorption-desorption cycle. Cu(II) accumulated mostly on the surface of the cell walls and was effectively desorbed by the acidic treatment during the desorption process

Index Entries

Magnetite-immobilized cells copper sorption and desorption mechanism transmission electron microscopy analysis growth phase Pseudomonas putida 5-x 


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  1. 1.
    Kratochvil, D. and Volesky, B. (1998), Water Res. 32, 2760–2768.CrossRefGoogle Scholar
  2. 2.
    Hong Kong Drainage Services Department. (1991), Technical Memorandum, Hong Kong Government Publisher, Hong Kong.Google Scholar
  3. 3.
    Sag, Y., Nourbakhsh, M., Aksu, Z., and Kutsal T. (1995), Proc. Biochem. 30, 175–181.Google Scholar
  4. 4.
    Chua, H. and Hua, F. L. (1996), Appl. Biochem. Biotechnol. 57, 845–849.CrossRefGoogle Scholar
  5. 5.
    Sag, Y. and Kutsal, T. (1995), Chem. Eng. J. 60, 181–188.Google Scholar
  6. 6.
    Volesky, B. and Holan, Z. R. (1995), Biotechnol. Prog. 11, 235–250.CrossRefGoogle Scholar
  7. 7.
    Volesky, B. (1994), FEMS Microbiol. Rev. 14, 291–302.CrossRefGoogle Scholar
  8. 8.
    Andres, Y., MacCordick, H. J., and Hubert, J. C. (1993), Appl. Microbiol. Biotechnol. 39, 413–417.CrossRefGoogle Scholar
  9. 9.
    Tsezos, M., Georgousis, Z., and Remoudaki, E. (1997), J. Chem. Technol. Biotechnol. 70, 198–206.CrossRefGoogle Scholar
  10. 10.
    Wong, P. K., Lam, K. C., and So, C. M. (1993), Appl Microbiol. Biotech. 39, 127–131.Google Scholar
  11. 11.
    Wong, P. K. and So, C. M. (1993), Microbiol. 73, 113–121.Google Scholar
  12. 12.
    Lu, Y. J., Chua, H., and Wong, P. K. (1998), Enz. Microb. Technol. 23, 403–407.CrossRefGoogle Scholar
  13. 13.
    Sze, K. F. (1996), PhD thesis, The Chinese University of Hong Kong, Hong Kong.Google Scholar
  14. 14.
    Hung, C. P. and Morehart, A. L. (1990), Water Res. 24, 433–439.CrossRefGoogle Scholar
  15. 15.
    Galun, M., Galun, E., Siegel, B. Z., Keller, P., Lehr, H., and Siegel, S. M. (1987), Water Air Soil Pollution 33, 359–371.CrossRefGoogle Scholar
  16. 16.
    He, L. M. and Tebo, B. M. (1998), Appl. Environ. Microbiol. 64, 1123–1129.Google Scholar
  17. 17.
    Pavel Kotrba, P., Doleckova, L., and de Lorenzo, V. (1999), Appl. Environ. Microbiol. 65, 1092–1098.Google Scholar
  18. 18.
    Lu, Y. M. and Wilkins, E. (1996), J. Hazardous Materials 49, 165–179.CrossRefGoogle Scholar
  19. 19.
    So, C. M. (1991), M. Phil. Thesis. The Chinese University of Hong Kong, Hong Kong.Google Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Wang Lei
    • 1
  • H. Chua
    • 1
  • W. H. Lo
    • 2
  • P. H. F. Yu
    • 2
  • Y. G. Zhao
    • 1
  • P. K. Wong
    • 3
  1. 1.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHung Hom, Hong KongChina
  2. 2.Department of Applied Biological and Chemical TechnologyHong Kong Polytechnic UniversityHung Hom, Hong KongChina
  3. 3.Department of BiologyThe Chinese University of Hong KongShatinChina

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