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Microbial Production of Polyhydroxyalkanoates by Bacteria Isolated from Oil Wastes

  • Ai Ling Wong
  • Hong Chua
  • Peter Hoi Fu Yu
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

A Gram-positive coccus-shaped bacterium capable of synthesizing higher relative molecular weight (M r) polyhydroxybutyrate (PHB) was isolated from sesame oil and identified as Staphylococcus epidermidis (by Microbial ID, Inc., Newark, NJ). The experiment was conducted by shake flask fermentation culture using media containing fructose. Cell growth up to a dry mass of 2.5 g/L and PHB accumulation up to 15.02% of cell dry wt was observed. Apart from using single carbohydrate as a sole carbon source, various industrial food wastes including sesame oil, ice cream, malt, and soya wastes were investigated as nutrients for S. epidermidis to reduce the cost of the carbon source. As a result, we found that by using malt wastes as nutrient for cell growth, PHB accumulation of S. epidermidis was much better than using other wastes as nutrient source. The final dried cell mass and PHB production using malt wastes were 1.76 g/L and 6.93% polymer/cells (grams/gram), and 3.5 g/L and 3.31% polymer/cells (grams/gram) in shake flask culture and in fermentor culture, respectively. The bacterial polymer was characterized by 1H-nuclear magnetic resonance (NMR), 13C-NMR, Fourier transform infrared, and differential scanning calorimetry. The results show that with different industrial food wastes as carbon and energy sources, the same biopolymer (PHB) was obtained. However, the use of sesame oil as the carbon source resulted in the accumulation of PHB with a higher melting point than that produced from other food wastes as carbon sources by this organism under similar experimental conditions.

Index Entries

Staphylococcus epidermidis polyhydroxybutyrate food wastes 

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References

  1. 1.
    Gassner, F. and Owen, A. J. (1996), Polymer Int. 39, 215–219.CrossRefGoogle Scholar
  2. 2.
    Lee, S. Y. (1996), Trends Biotechnol. 14, 431–438.CrossRefGoogle Scholar
  3. 3.
    Anderson, A. J. and Dawes, E. A. (1990), Microbiol. Rev. 54, 450–472.Google Scholar
  4. 4.
    Steninbuchel, A. and Schlegel, H. G. (1991), Mol. Microbiol. 5(3), 535–542.CrossRefGoogle Scholar
  5. 5.
    Doi, Y. (1990), Microbial Polyesters, VCH Publishers, New York.Google Scholar
  6. 6.
    Ceccorulli, G., Pizzoli, M., and Scandola, M. (1992), Macromolecules 25, 3304–3306.CrossRefGoogle Scholar
  7. 7.
    Abe, H., Doi, Y., Aoki, H., and Akehata, T. (1998), Macromolecules 31, 1791–1797.CrossRefGoogle Scholar
  8. 8.
    Müler, R. J., Witt, U., Rantze, E., and Deckwer, W. D. (1998), Polymer Degradation Stabil. 59, 203–208.CrossRefGoogle Scholar
  9. 9.
    Labuzek, S., Radecka, I., and Kowalczuk, M. (1994), Biol. Sci. 42(2), 121–123.Google Scholar
  10. 10.
    Lee, S. Y. (1996), Biotechnol. Bioeng. 49, 1–14.CrossRefGoogle Scholar
  11. 11.
    Yu, P. H., Chua, H., Huang, A. L., Lo, W., and Chen, G. Q. (1998), Appl. Biochem. Biotechnol. 70-72, 603–614.CrossRefGoogle Scholar
  12. 12.
    Yu, P. H., Chua, H., Huang, A. L., and Ho, K. P. (1999), Appl. Biochem. Biotechnol. 77-79, 445–454.CrossRefGoogle Scholar
  13. 13.
    Atlas, R. M. (1997), Handbook of Microbiological Media, CRC Press, Boca Raton, FL.Google Scholar
  14. 14.
    Cromwick, A. M., Foglia, T., and Lenz, R. W. (1996), Appl. Microbiol. Biotechnol. 46, 464–469.CrossRefGoogle Scholar
  15. 15.
    Jan, S., Roblot, C., Goethals, G., Courtois, J., Courtois, B., Saucedo, J. E. N., Seguin, J. P., and Barbotin, J. N. (1995), J. Anal. Biochem. 225, 258–263.CrossRefGoogle Scholar
  16. 16.
    Jan, S., Roblot, C., Courtois, J., Courtois, B., Barbotin, J. N., and Seguin, J. P. (1996), J. Enzyme Microb. Technol. 18, 195–201.CrossRefGoogle Scholar
  17. 17.
    Bloembergen, S., Holden, D. A., Hamer, G. K., Bluhm, T. L., and Marchessault, R. H. (1986), Macromolecules 19, 2865–2871.CrossRefGoogle Scholar
  18. 18.
    Zhang, L., Deng, X., Zhao, S., and Huang, Z. (1997), Polymer 38(24), 6001–6007.CrossRefGoogle Scholar
  19. 19.
    Akita, S., Einaga, Y., Miyaki, Y., and Fujita, H. (1976), Macromolecules 9, 774–780.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Ai Ling Wong
    • 1
  • Hong Chua
    • 2
  • Peter Hoi Fu Yu
    • 1
  1. 1.Open Laboratory of Chirotechnology and Department of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityHung Hom, KowloonChina
  2. 2.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHung Hom, KowloonChina

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