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Salinity-Regulated Replication of the Endogenous Plasmid pSY10 from the Marine Cyanobacterium Synechococcus sp.

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Book cover Twenty-First Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

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Abstract

The endogenous plasmid pSY10 in the marine cyanobacterium Synechococcus sp. NKBG042902 is maintained at a high copy number when cells are grown in seawater and at a low copy number when cultured in freshwater. The mechanism of salinity-regulated replication of this plasmid was investigated. Transcription of repA was depressed under freshwater, which was accompanied by a low copy number of pSY10 and the appearance of a new protein that was expressed only in cells cultured in freshwater. This protein was observed to bind to putative repA promoters (Prep1 and Prep2) on pSY10. Moreover, this protein was observed only in Synechococcus sp. NKBG042902. The data suggest that this protein(s) regulates repA transcription in pSY10, stress responsive and encoded by the host chromosome.

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-855, Japan

    Haruko Takeyama, Hideki Nakayama & Tadashi Matsunaga

Authors
  1. Haruko Takeyama
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  2. Hideki Nakayama
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  3. Tadashi Matsunaga
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Corresponding author

Correspondence to Tadashi Matsunaga .

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Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

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© 2000 Springer Science+Business Media New York

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Takeyama, H., Nakayama, H., Matsunaga, T. (2000). Salinity-Regulated Replication of the Endogenous Plasmid pSY10 from the Marine Cyanobacterium Synechococcus sp.. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_36

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  • DOI: https://doi.org/10.1007/978-1-4612-1392-5_36

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7128-4

  • Online ISBN: 978-1-4612-1392-5

  • eBook Packages: Springer Book Archive

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