Abstract
An indium tin oxide (ITO) electrode was chemically modified by one layer of viologen (VIO) derivative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hydrogenase from Thiocapsa roseopersicina was stabilized on a synthesized poly-L-lysine subphase surface and transferred onto the electrode for fabrication of an ITO-VIO-hydrogenase heterogeneous system. Electrochemical properties of both the ITO-VIO monolayer and the heterogeneous ITO-VIO-hydrogenase system have been investigated. Hydrogen evolution could be measured by potentiostating the VIO-hydrogenase-covered ITO electrode to “electroplate” [(VIO+)n]surf, and a large increase in hydrogen evolution was observed when using an electrolyte solution containing sodium dithionite. We discuss the possible electron transfer process.
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References
Zaborsky, O. R. (1998), BioHydrogen, Plenum, New York.
Miyake, J. (1998), in BioHydrogen, Zaborsky, O. R., ed., Plenum, New York pp. 7–18.
Fernández, V. M. (1983), Anal. Biochem. 130, 54–59.
Kovacs, K. L. and Der, A. (1986), Biochimie 68, 211–215.
McTavish, H. (1998), J. Biochem. 123, 644–649.
Bird, C.-L. and Kuhn, A. T. (1981), Chem. Soc. Rev. 10, 49–82.
Kaganer, E., Joselevich, E., Willner, I., Chen, Z., Gunter, M. J., Gayness, T. P., and Johnson, M. R. (1998), J. Phys. Chem. B 102, 1159–1165.
Noda, K., Zorin, N. A., Nakamura, C., Miyake, M., Gogotov, I. N., Asada, Y., Akutsu, H., and Miyake, J. (1998), Thin Solid Films 327-329, 639–642.
Kutner, W., Wang, J., L’her, M., and Buck, R. P. (1998), Pure Appl. Chem. 70, 1301–1318.
Cea, P., Lafuente, C., Urieta, J. S., Lopez, M. C., and Royo, F. M. (1998), Langmuir 14, 7306–7312.
Dong, S. and Li, J. (1997), Bioelectrochemistry and Bioenergetics 42, 7–13.
Bookbinder, D. C. and Wrighton, M. S. (1980), J. Am. Chem. Soc. 102, 5123–5125.
Palmore, G.T.R., Smith, D. K., and Wrighton, M. S. (1997), J. Phys. Chem. B 101, 2437–2450.
Sherman, M. B., Orlova, E. V., Smirnova, E. A., Hovmöller, S., and Zorin, N. A. (1991), J. Bacteriol. 173, 2576–2580.
Gogotov, I. N., Zorin, N. A., Serebriakova, L. T., and Kondratieva, E. N. (1978), Biochim. Biophys. Acta, 523, 335–343.
Zorin, N. A., Pashkova, O. N., and Gogotov, I. N. (1995), Biochemistry (Moscow) 60, 379–384.
Dominey, R. N., Lewis, T. J., and Wrighton, M.S. (1983), J. Phys. Chem. 87, 5345–5354.
Taniguchi, T., Fukasawa, Y., and Miyashita, T. (1999), J. Phys. Chem. B. 103, 1920–1924.
Shimomura, M., Kasuga, K., and Tsukada, T. (1992), Thin Solid Films 210/211, 375–377.
Tang, X., Schneider, T. W., Walker, J. W., and Buttry, D. A. (1996), Langmuir 12, 5921–5933.
Qian, D.-J., Nakamura, C., and Miyake, J., submitted.
Lee, C.-W. and Bard, A. J. (1988), J. Electroanal. Chem. 239, 441–446.
Ulman, A. (1991), An Introduction to Ultrathin Organic Films, Academic, Boston, MA.
Haverkamp, G. K, Ranke, H., and Friedrich, C. G. (1995), Appl. Microbiol. Biotechnol. 44, 514–518.
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Qian, DJ., Nakamura, C., Noda, K., Zorin, N.A., Miyake, J. (2000). Fabrication of an Electrode-Viologen-Hydrogenase Heterogeneous System and the Electrochemical Hydrogen Evolution. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_32
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DOI: https://doi.org/10.1007/978-1-4612-1392-5_32
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