Advertisement

Floating Cultivation of Marine Cyanobacteria Using Coal Fly Ash

  • Mitsufumi Matsumoto
  • Eri Yoshida
  • Haruko Takeyama
  • Tadashi Matsunaga
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

The aim of this study was to develop improved methodologies for bulk culturing of biotechnologically useful marine cyanobacteria in the open ocean. We have investigated the viability of using coal fly ash (CFA) blocks as the support medium in a novel floating culture system for marine microalgae. The marine cyanobacterium Synechococcus sp. NKBG 040607 was found to adhere to floating CFA blocks in liquid culture medium. Maximum density of attached cells of 2.0 x 108 cells/cm2 was achieved using seawater. The marine cyanobacterium Synechococcus sp. NKBG 042902 weakly adhered to floating CFA blocks in BG-11 medium. Increasing the concentration of calcium ion in the culture medium enhanced adherence to CFA blocks.

Index Entries

Marine cyanobacteria floating culture coal fly ash immobilization power plant calcium ion 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Benemann, J. R., Tillet, D. M., and Weissaman, J. C. (1987), Trends. Biotechnol. 5, 47–53.CrossRefGoogle Scholar
  2. 2.
    Burgess, J. G., Iwamoto, K., Miura, Y., Takano, H., and Matsunaga, T. (1993), Appl. Microbiol. Biotechnol. 39, 456–459.CrossRefGoogle Scholar
  3. 3.
    Matsunaga, T., Burgess, J. G., Yamada, N., Komatsu, K., Yoshida, S., and Wachi, Y. (1993), Appl. Microbiol. Biotechnol. 39, 250–253.Google Scholar
  4. 4.
    Takano, H., Takeyama, H., Nakamura, N., Sode, K., Burgess, J. G., Manabe, E., Hirano, M., and Matsunaga, T. (1992), Appl. Biochem. Biotechnol. 34/35, 449–458.CrossRefGoogle Scholar
  5. 5.
    Takano, H., Takei, R., Manabe, H., Burgess, J. G., Hirano, M., and Matsunaga, T. (1995), Appl. Microbiol. Biotechnol. 43, 460–465.CrossRefGoogle Scholar
  6. 6.
    Miura, Y., Sode, K., Nakanura, N., Matsunaga, N., and Matsunaga, T. (1993), FEMS Microbiol. Lett. 107, 163–168.CrossRefGoogle Scholar
  7. 7.
    Wake, H., Akasaka, A., Umetsu, H., Ozeki, Y., Shimomura, K., and Matsunaga, T. (1992), Appl. Microbiol. Biotechnol. 36, 684–688.CrossRefGoogle Scholar
  8. 8.
    Matsunaga, T., Takeyama, H., and Takano, H. (1999), The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation, John Wiley & Sons, New York.Google Scholar
  9. 9.
    Scott, C., Fletcher, R. L., and Bremer, G. B. (1996), Biofouling 10, 161–173.CrossRefGoogle Scholar
  10. 10.
    Wastman, B. A., Lind, J., Wetherbee, R., and Gretz, M. R. (1998), Plant. Physiol. 116, 1431–1441.CrossRefGoogle Scholar
  11. 11.
    Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., and Stanier, R. Y. (1979), J. Gen. Microbiol. 111, 1–61.Google Scholar
  12. 12.
    Eppley, R. W., Holmes, R. W., and Strickland, J. D. H. (1967), J. Exp. Mar. Biol. Ecol. 1, 191–208.CrossRefGoogle Scholar
  13. 13.
    Sudo, H., Yamada, A., Kokatsu, K., Nakamura, N., and Matsunaga, T. (1997), Appl. Microbiol. Biotechnol. 47, 78–82.CrossRefGoogle Scholar
  14. 14.
    Ayoub, G. M., Lee, Sang, I. L. L., and Koopman, B. (1986), Water Res. 20, 1265–1271.CrossRefGoogle Scholar
  15. 15.
    Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., and Borner, T. (1999), J. Bacteriol. 181, 4089–4097.Google Scholar
  16. 16.
    Tsuji, K., Watanuki, T., Kondo, F., Watanabe, M. F., Nakazawa, H., Suzuki, M., Uchida, H., and Harada, K. (1997), Toxicon 35, 1033–1041.CrossRefGoogle Scholar
  17. 17.
    Rapala, J., Sivonen, K., Lyra, C., and Niemela, S. I. (1997), Appl. Environ. Microbiol. 63, 2206–2212.Google Scholar
  18. 18.
    Matsunaga, T., Takeyama, H., Sudo, H., Oyama, N., Ariura, S., Takano, H., Hirano, M., Burgess, J. G., Sode, K., and Nakamura, N. (1991), Appl. Biochem. Biotechnol. 28/29, 157–167.CrossRefGoogle Scholar
  19. 19.
    Matsunaga, T., Nakamura, N., Tsuzuki, N., and Takeda, H. (1988), Appl. Microbiol. Biotechnol. 28, 373–376.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Mitsufumi Matsumoto
    • 1
  • Eri Yoshida
    • 1
  • Haruko Takeyama
    • 1
  • Tadashi Matsunaga
    • 1
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan

Personalised recommendations