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Endocellulase and Exocellulase Activities of Two Streptomyces Strains Isolated from a Forest Soil

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Book cover Twenty-First Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

Two Streptomyces strains, M7a and M23, from a Brazilian forest soil were evaluated for the cellulase production of their supernatants after growth in a microcrystalline cellulose medium, using carboxymethylcellulose and filter paper as substrates at different temperatures and pH values. Endoglucanase and exoglucanase activities were compared to a commercial Trichoderma reesei cellulase using fluorogenic conjugated substrates. Similar specific activities were observed for the enzyme preparations of strain M23 and T. Reesei. For M7a the activities were about seven times higher than those obtained for T. Reesei. Extracellular or cell-associated cellobiase activities were not detected in both strains.

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Author information

Authors and Affiliations

  1. Instituto de Microbiologia Prof. Paulo de Góes-UFRJ, CCS, Bloco I, Ilha do Fundão, Rio de Janeiro, CEP 21490 021, Brazil

    Luzia T. A. S. Semêdo, Rosana C. Gomes, Rosangela M. A. Soares, Luiz F. Linhares & Rosalie R. R. Coelho

  2. Instituto de Química-UFRJ, C.T., Bloco A, Ilha do Fundão, Rio de Janeiro, CEP 21949 900, Brazil

    Elba P. S. Bon

Authors
  1. Luzia T. A. S. Semêdo
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  2. Rosana C. Gomes
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  3. Elba P. S. Bon
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  4. Rosangela M. A. Soares
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  5. Luiz F. Linhares
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  6. Rosalie R. R. Coelho
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Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

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© 2000 Springer Science+Business Media New York

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Semêdo, L.T.A.S., Gomes, R.C., Bon, E.P.S., Soares, R.M.A., Linhares, L.F., Coelho, R.R.R. (2000). Endocellulase and Exocellulase Activities of Two Streptomyces Strains Isolated from a Forest Soil. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_20

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  • DOI: https://doi.org/10.1007/978-1-4612-1392-5_20

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7128-4

  • Online ISBN: 978-1-4612-1392-5

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