Abstract
Clostridium beijerinckii BA101 (mutant strain) and C. beijerinckii 8052 (wild type) were compared for substrate and butanol inhibition. The wild-type strain is more strongly inhibited by added butanol than is the mutant strain. Acetone and butanol were removed from a fed-batch reactor inoculated with C. beijerinckii BA101 by pervaporation using a silicone membrane. In the batch reactor, C. beijerinckii BA101 produced 25.3 g/L of total solvents, whereas in the fermentation-recovery experiment it produced 165.1 g/L of total solvents. Solvent productivity increased from 0.35 (batch reactor) to 0.98 g/L.h (fed-batch reactor). The fed-batch reactor was fed with 500 g/L of glucose-based P2 medium. Acetone selectivities ranged from 2 to 10 whereas butanol selectivities ranged from 7 to 19. Total flux varied from 26 to 31 g/m2.h.
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References
Woods, D. T. and Woods, D. R. (1986), Microbiol. Rev. 50, 484–524.
McNeil, B. and Kristiansen, B. (1986), Adv. Appl. Microbiol. 31, 61–92.
Maddox, I. S. (1989), Biotechnol. Genet. Eng. Rev. 7, 189–220.
Parekh, M., Formanek, J., and Blaschek, H. P. (1998), J. Ind. Microbiol. Biotechnol. 21, 187–191.
Annous, B. A. and Blaschek, H. P. (1991), Appl. Environ. Microbiol. 57, 2544–2548.
Formanek, J., Mackie, J. R., and Blaschek, H. P. (1997), Appl. Environ. Microbiol. 63, 2306–2310.
Chen, C.-K. and Blaschek, H. P. (1999), Appl. Microbiol. Biotechnol. 52, 170–173.
Wagner, J. J., Lusby, K. S., and Horn, G. W. (1983), J. Anim. Sci. 57, 542–552.
Parekh, M., Formanek, J., and Blaschek, H. P. (1999), Appl. Microbiol. Biotechnol. 51, 152–157.
Qureshi, N., Maddox, I. S., and Friedl, A. (1992), Biotechnol. Prog. 8, 382–390.
Qureshi, N. (1997), PhD thesis, University of Nebraska, Lincoln, NE.
Qureshi, N. and Blaschek, H. P. (1999), Separation Sci. Technol. 34, 2803–2815.
Qureshi, N. and Blaschek, H. P. (1999), Biotechnol. Prog. 15, 594–602.
Qureshi, N. and Blaschek, H. P. (1999), Biomass Bioenergy 17, 175–184.
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Qureshi, N., Blaschek, H.P. (2000). Butanol Production Using Clostridium beijerinckii BA101 Hyper-Butanol Producing Mutant Strain and Recovery by Pervaporation. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_16
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DOI: https://doi.org/10.1007/978-1-4612-1392-5_16
Publisher Name: Humana Press, Totowa, NJ
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