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Twenty-First Symposium on Biotechnology for Fuels and Chemicals pp 201–216Cite as

Characterization and Complementation of a Pichia stipitis Mutant Unable to Grow on D-Xylose or L-Arabinose

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Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

Pichia stipitis CBS 6054 will grow on D-xylose, D-arabinose, and L-arabinose. D-Xylose and L-arabinose are abundant in seed hulls of maize, and their utilization is important in processing grain residues. To elucidate the degradation pathway for L-arabinose, we obtained a mutant, FPL-MY30, that was unable to grow on D-xylose and L-arabinose but that could grow on D-arabinitol. Activity assays of oxidoreductase and pentulokinase enzymes involved in D-xylose, D-arabinose, and L-arabinose pathways indicated that FPL-MY30 is deficient in D-xylitol dehydrogenase (D-XDH), D- and L-arabinitol dehydrogenases, and D-ribitol dehydrogenase. Transforming FPL-MY30 with a gene for xylitol dehydrogenase (PsXYL2), which was cloned from CBS 6054 (GenBank AF127801), restored the D-XDH activity and the capacity for FPL-MY30 to grow on L-arabinose. This suggested that FPL-MY30 is critically deficient in XYL2 and that the D-xylose and L-arabinose metabolic pathways have xylitol as a common intermediate. The capacity for FPL-MY30 to grow on D-arabinitol could proceed through D-ribulose.

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Author notes

  1. Kristine Prahl

    Present address: University of Wisconsin-Marathon County, 518 S. 7th Avenue, Wausau, WI, 54401, USA

  2. Jim Hendrick

    Present address: Ophidian Pharmaceuticals Inc., 5445 E. Cheryl Parkway, Madison, WI, 53711, USA

  3. Jose Cruz

    Present address: Department of Chemical Engineering, University of Vigo, As Lagoas, 32004, Ourense, Spain

  4. Ping Lu

    Present address: Merck, P.O. Box 2000, RY80Y-125, Rahway, NJ, 07065, USA

  5. Jae-Yong Cho

    Present address: NCI-Frederick Cancer Research and Development Center, Frederick, MD, 21701, USA

  6. Sharon Jones

    Present address: Department of Anatomy, University of Wisconsin, Madison, WI, 53706, USA

Authors and Affiliations

  1. Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI, 53706, USA

    Nian-Qing Shi, Ping Lu & Jae-Yong Cho

  2. Department of Biochemistry, University of Wisconsin, 420 Henry Mall, Madison, WI, 53706, USA

    Kristine Prahl

  3. Forest Service, Forest Products Laboratory, USDA, 1 Gifford Pinchot Drive, Madison, WI, 53705, USA

    Jim Hendrick, Jose Cruz, Sharon Jones & Thomas Jeffries

Authors
  1. Nian-Qing Shi
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  2. Kristine Prahl
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  4. Jose Cruz
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  5. Ping Lu
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  6. Jae-Yong Cho
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  7. Sharon Jones
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  8. Thomas Jeffries
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Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

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© 2000 Springer Science+Business Media New York

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Shi, NQ. et al. (2000). Characterization and Complementation of a Pichia stipitis Mutant Unable to Grow on D-Xylose or L-Arabinose. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_14

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  • DOI: https://doi.org/10.1007/978-1-4612-1392-5_14

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7128-4

  • Online ISBN: 978-1-4612-1392-5

  • eBook Packages: Springer Book Archive

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