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Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number

  • Scott J. Brodie
  • Patricia M. O’Hearn
  • Kurt Diem
  • David Muthui

Abstract

Orbivirus is a genus in the family Reoviridae and includes epizootic hemorrhagic disease viruses (EHDV), bluetongue viruses (BTV), and African horses sickness viruses. All members of this genus have an arthropod vector, replicate in the cytoplasm of infected cells, and have a double-layered protein capsid consisting of seven polypeptides, each of which is encoded by one of ten double-stranded RNA viral segments [1]. The outcome of orbivirus infection varies, ranging from subclinical or mild disease to acute and fatal disease. Acute disease, as seen in sheep and some wild ruminants, is characterized by inflammation, hemorrhage, and/or necrosis of mucosal surfaces in the oronasal and alimentary systems. Animals that survive infection may develop chronic dermatitis and vesicular and/or erosive lesions at interdigital and mucosal surfaces [2].

Keywords

Gene Segment Viral Nucleic Acid Mononuclear Leukocyte Wild Ruminant Bluetongue Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Calisher CH. Medically important arboviruses of the United States and Canada. Clin Microbiol Rev 1995; 7: 89–116.Google Scholar
  2. 2.
    Brewer AW, MacLachlan NJ. The pathogenesis of bluetongue virus infection of bovine blood cells in vitro: ultrastructural characterization. Arch Virol 1994; 136: 287–298.PubMedCrossRefGoogle Scholar
  3. 3.
    Aradaib IE, Akita GY, Pearson JE, Osburn BI. Comparison of polymerase chain reaction and virus isolation for detection of epizootic hemorrhagic disease virus in clinical samples from naturally infected deer. J Vet Diagn Invest 1995: 7: 196–200.PubMedCrossRefGoogle Scholar
  4. 4.
    Aradaib IE, McBride JW, Wilson WC, Osburn BI. Development of polymerase chain reaction for specific identification of epizootic hemorrhagic disease virus serotype 1. Arch Virol 1995; 140: 2273–2281.PubMedCrossRefGoogle Scholar
  5. 5.
    Aradaib IE, Wilson WC, Cheney IW, Pearson JE, Osburn BI. Application of PCR for specific identification of epizootic hemorrhagic disease virus serotype 2. J Vet Diagn Invest 1995; 7: 388–392.PubMedCrossRefGoogle Scholar
  6. 6.
    Shad G, Wilson WC, Mecham JO, Evermann JF. Bluetongue virus detection: a safer reverse-transcriptase polymerase chain reaction for prediction of viremia in sheep. J Vet Diagn Invest 1997; 9: 118–124.PubMedCrossRefGoogle Scholar
  7. 7.
    Brodie SJ, Bardsley KD, Mecham JO, Diem K, Norelius SE, Wilson WC. Epizootic hemorrhagic disease: analysis of tissues by amplification and in situ hybridization reveals widespread orbivirus infection at low copy number. J Virol 1998; 72: 3863–3871.PubMedGoogle Scholar
  8. 8.
    Brodie SJ, Wilson WC, O’Hearn PM, Muthui D, Diem K, Snowder GD, Pearson LD. The effects of pharmacological and lentivirus-induced immune suppression on orbivirus pathogenesis: assessment of virus burden in blood monocytes and tissues by reverse transcription in situ PCR. J Virol 1998; 72: 5599–5609.PubMedGoogle Scholar
  9. 9.
    MacLachlan NJ, Nunamaker RA, Katz JB, Sawyer MM, Akita GY, Osburn BI, Tabachnick WJ. Detection of bluetongue virus in the blood of inoculated calves: comparison of virus isolation, PCR assay, and in vitro feeding of Culicoides variipennis. Arch Virol 1994; 136: 1–8.PubMedCrossRefGoogle Scholar
  10. 10.
    Brown CC, Meyer RF, Grubman MJ. Use of a digoxigenin-labeled–RNA probe to detect all 24 serotypes of bluetongue virus in cell culture. J Vet Diagn Invest 1993; 5: 159–162.PubMedCrossRefGoogle Scholar
  11. 11.
    Dangler CA, de la Concha-Bermejillo A, Stott JL, Osburn BI. Limitations of in situ hybridization for the detection of bluetongue virus in blood mononuclear cells. J Vet Diag Invest 1990; 2: 303–307.CrossRefGoogle Scholar
  12. 12.
    Venter EH, Viljoen GJ, Nel LH, Huismans H, van Dijk AA. A comparison of different genomic probes in the detection of virus-specific RNA in Orbivirusinfected cells. J Virol Methods 1991; 32: 171–180.PubMedCrossRefGoogle Scholar
  13. 13.
    de la Concha-Bermejillo A, Schore CE, Dangler CA, de Mattos CC, de Mattos CA, Osburn BI. Comparison of slot blot nucleic acid hybridization, immunofluorescence, and virus isolation techniques to detect bluetongue virus in blood mononuclear cells from cattle with experimentally induced infection. Am J Vet Res 1992; 53: 2245–2250.Google Scholar
  14. 14.
    Schnittman SM, Psallidopoulos MC, Lane HC, Thompson L, Baseler M, Massari F, Fox CH, Salzman NP, Fauci AS. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4. Science 1993; 245: 305–308.CrossRefGoogle Scholar
  15. 15.
    Embretson J, Zupancic M, Beneke J, Till M, Wolinsky S, Ribas JL, Burke A, Haase AT. Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single-cell resolution. Proc Natl Acad Sci USA 1993; 90: 357361.Google Scholar
  16. 16.
    Patterson BK, Till M, Otto P, Goolsby C, Furtado MR, McBride LJ, Wolinsky SM. Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry. Science 1993; 260: 976979.Google Scholar
  17. 17.
    Bagasara O, Pomerantz RJ. In situ polymerase chain reaction and HIV-1. Clin Lab Med 1994; 14: 351–365.Google Scholar
  18. 18.
    Brodie SJ, Marcom KA, Pearson LD, Anderson BC, de la Concha-Bermejillo A, Ellis JA, DeMartini JC. The effects of virus load in the pathogenesis of lentivirus-induced lymphoid interstitial pneumonia. J Infect Dis 1992; 166: 531–541.PubMedCrossRefGoogle Scholar
  19. 19.
    Wilson WC. Development of a nested-PCR test based on sequence analysis of epizootic hemorrhagic disease viruses non-structural protein 1 (NS1). Virus Res 1994; 31: 357–365.PubMedCrossRefGoogle Scholar
  20. 20.
    Cottler-Fox M, Fox CH. Examining cells for infectious agents: a novel approach. J Infect Dis 1991; 161: 1239–1240.CrossRefGoogle Scholar
  21. 21.
    Holland JJ, Spindler K, Horodyski F, Grabau E, Nichol S, VandePol S. Rapid evolution of RNA genomes. Science 1982; 215: 1577–1585.PubMedCrossRefGoogle Scholar
  22. 22.
    Domingo E, Martinez-Salas E, Sobrino F, de la Torre JC, Portela A, Ortin J, Lopez-Galindez C, Perez-Brena P, Villaneuva N, Najera R, VandePol S, Steinhauer D, DePolo N, Holland J. The quasispecies (extremely heterologous) nature of viral RNA genome populations: biological relevance-a review. Gene 1985; 40: 1–8.PubMedCrossRefGoogle Scholar
  23. 23.
    MacLachlan NJ, Jagels G, Rossitto PV, Moore PF, Heidner H. The pathogenesis of experimental bluetongue virus infection of calves. Vet Pathol 1990; 27: 223–229.PubMedGoogle Scholar
  24. 24.
    Coen ML, Ellis JA, O’Toole DT, Wilson WC. Cytokine modulation of the interaction between bluetongue virus and endothelial cells in vitro. Vet Pathol 1991; 28: 524–532.PubMedCrossRefGoogle Scholar
  25. 25.
    Grubman MJ, Samal S. Nucleotide and deduced amino acid sequence of the nonstructural protein, NS1, of the U.S. bluetongue virus serotype 17. Nucleic Acids Res 1989; 17: 104–98.CrossRefGoogle Scholar
  26. 26.
    Nel LH, Picard LA, Huismans H. A characterization of the nonstructural protein from which the virus-specific tubules in epizootic haemorrhagic disease virus-infected cells are composed. Virus Res 1990; 18: 219–230.CrossRefGoogle Scholar
  27. 27.
    Wilson WC. Sequence analysis of the non-structural protein 2 from epizootic hemorrhagic disease viruses. Virus Res 1994; 34: 63–68.PubMedCrossRefGoogle Scholar
  28. 28.
    Jensen MJ, Wilson WC. A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1. Arch Virol 1995; 140: 799–805.PubMedCrossRefGoogle Scholar
  29. 29.
    Unger RE, Chuang RY, Chuang LF, Osburn BI, Doi RH. The cloning of full-length genome segments 2, 5, 6 and 8 of bluetongue (BTV) serotype 17 and studies of their genetic relatedness to United States BTV serotypes. Virology 1988; 167: 296–298.Google Scholar
  30. 30.
    Urakawa T, Roy P. Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10. J Virol 1988; 62: 3919–3927.PubMedGoogle Scholar
  31. 31.
    Huismans H, Els HJ. Characterization of the tubules associated with the replication of three different orbiviuses. Virology 1979; 92: 397–406.PubMedCrossRefGoogle Scholar
  32. 32.
    Huismans H, Verwoerd DW. Control of transcription during the expression of the bluetongue virus genome. Virology 1973; 52: 81–88.PubMedCrossRefGoogle Scholar
  33. 33.
    Schoepp RJ, Blair CD, Roy P, Beaty BJ. Detection of bluetongue virus RNA by in situ hybridization: comparison with virus isolation and antigen detection. J Vet Diag Invest 1991; 3: 22–28.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Scott J. Brodie
  • Patricia M. O’Hearn
  • Kurt Diem
  • David Muthui

There are no affiliations available

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