Abstract
The preservation of tissues dates back to the early Egyptians, and the use of aldehyde fixatives does not seem far behind in antiquity. The nineteenth century, at very least, was a period in which the biochemical mechanisms of tissue preservation and tissue analysis were defined. Since that time, DNA was discovered, and now the entire human genome has been mapped. In this era of molecular basis of disease, we must examine the goals of tissue preservation in a different vein. Optimally, clinicians and scientists desire a fixative that would maintain perfect cellular morphology and tissue architecture while simultaneously preserving RNA, DNA, and proteins in their unfixed state. This chapter will describe individual fixatives that approach this holy grail, but most importantly it will describe cell and tissue banking strategies that will not exclude current analytical technologies.
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Patterson, B.K., Jiyamapa, D.K. (2000). Fixation for In Situ Molecular Analysis. In: Patterson, B.K. (eds) Techniques in Quantification and Localization of Gene Expression. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-1342-0_2
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DOI: https://doi.org/10.1007/978-1-4612-1342-0_2
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-1-4612-7103-1
Online ISBN: 978-1-4612-1342-0
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