Abstract
The key to immunoassay success is analytical sensitivity and specificity, which in turn lead to clinical utility. The immunoassay reagent, the antibody, has the characteristic of being able to bind tightly and relatively specifically to the invading agent (the antigen) and thereafter initiate a series of events terminating in the biological neutralization of the invading agent. It is this binding event that gives immunoassays their importance in clinical medicine. The reaction between the selected antibody and its target antigen is generally highly specific, rapid, and effectively irreversible. Thus, using antibodies as reagents in a testing system should allow the specific detection of relatively small amounts of antigen from mixtures of generically similar materials. For example, thyroid stimulating hormone can be determined at 10-12mol/L in the presence of greater than approx 108-fold other proteins that are essentially physically identical(1)
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Place, J.F., Sutherland, R.M., Riley, A., Mangan, C. (1991). Immunoassay Kinetics at Continuous Surfaces. In: Wise, D.L., Wingard, L.B. (eds) Biosensors with Fiberoptics. Contemporary Instrumentation and Analysis. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0483-1_8
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DOI: https://doi.org/10.1007/978-1-4612-0483-1_8
Publisher Name: Humana Press, Totowa, NJ
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