Applicability of Microwave Acid Digestion to Sample Preparation of Biological Materials for Analysis by Particle-Induced X-Ray Emission (PIXE)

  • T. Pinheiro
  • H. Duflou
  • W. Maenhaut


A microwave add digestion method for the preparation of biological samples for PIXE analysis is presented. The precision and accuracy of the entire PIXE analytical procedure, including the microwave digestion step, were evaluated by analyzing eight certified reference materials. For elements heavier than K, and for concentration levels from 2 jxg/g upward, the total random error of a single analysis is in the range of 2 –5%. The accuracy is better than 5%. The detection limits are down to 0.3 μg/g.

Index Entries

Particle-induced X-ray emission PIXE sample preparation microwave digestion acid digestion trace elements reference materials precision accuracy detection limits 


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  1. 1.
    S. A. E. Johansson and J. IL Campbell, PIXE: A Novel Technique for Elemental Analysis, Wiley, Chichester (1988).Google Scholar
  2. 1.
    W. Maenhaut, Nucl. Instr. Methods B35, 388 (1988).Google Scholar
  3. 3.
    N. F. Mangelson, M, W. Hill, K. K. Nielson, and J. F. Ryder, Nucl. Instr. Methods 142, 133 (1977).CrossRefGoogle Scholar
  4. 4.
    W. Maenhaut, L. De Reu, H. A, Van Rinsvelt, J. Cafmeyer, and P. Van Espen, Nucl. Instr. Methods 168, 557 (1980).CrossRefGoogle Scholar
  5. 5.
    J. Pallon and K. G. Malmqvist, Nucl. Instr. Methods 181, 71 (1981).CrossRefGoogle Scholar
  6. 6.
    W. Maenhaut, L. De Reu, and J. Vandenhaute, Nucl. Instr. Methods B3,135 (1984).Google Scholar
  7. 7.
    J. Galuszka, L. Jarczyk, E. Rokita, A. Strzalkowski, and M. Sych, Nucl. Instr. Methods B3, 141 (1984).Google Scholar
  8. 8.
    E. Clayton, Nucl. Instr. Methods B22, 145 (1987).Google Scholar
  9. 9.
    W, Maenhaut, J. Vandenhaute, and H. Duflou, Fresenius Z. Anal. Chem. 326, 736 (1987).CrossRefGoogle Scholar
  10. 10.
    H. Duflou, W. Maenhaut, and J. De Reuck, Biol Trace Elem. Res. 13,1 (1987).Google Scholar
  11. 11.
    H. M. Kingston and L. B. Jassie, Introduction to Microwave Sample Preparation, ACS Professional Reference Book, Washington (1988).Google Scholar
  12. 12.
    H. Duflou, PhD thesis, University of Ghent, 1988.Google Scholar
  13. 13.
    W. Maenhaut and H. Raemdonck, Nucl. Instr. Methods B1, 123 (1984).Google Scholar
  14. 14.
    W. Maenhaut and J. Vandenhaute, Bull Soc. Chim. Belg. 95, 407 (1986).CrossRefGoogle Scholar
  15. 15.
    E. S. Gladney, C. E. Burns, D. R. Perrin, I. Roelandts, and T. E. Gills, 1982 Compilation of Elemental Concentration Data for NBS Biological, Geological, and Environmental Standard Reference Materials, NBS (US) Spec. Publ. 260-88 (1984).Google Scholar
  16. 16.
    J. L. Campbell, W. Maenhaut, E. Bombelka, E. Clayton, K. Malmqvist, J. A. Maxwell, J. Pallon, and J. Vandenhaute, Nucl. Instr. Methods B14,204 (1986).Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • T. Pinheiro
    • 1
    • 2
  • H. Duflou
    • 2
  • W. Maenhaut
    • 2
  1. 1.Physics DepartmentIAEA Fellow, Permanent affiliation —LNETISacavemPortugal
  2. 2.Instituut voor Nucleaire WetenschappenRijksuniversiteit GentGentBelgium

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