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Structure-Function Relationships

  • Chapter
The Beta-Adrenergic Receptors

Part of the book series: The Receptors ((REC))

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Abstract

Understanding, in molecular terms, the ways in which extracellular signals are transduced across the cell membrane so as to modify key intracellular metabolic processes ultimately requires that the various elements in the pathway be isolated and their structures determined. Only then can the structural basis for such signaling systems be understood. Of the many receptors that are coupled through guanine nucleotide regulatory proteins to various cellular effectors (enzymes, ion channels), perhaps the most throughly studied has been the beta-adrenergic receptor. Moreover, much has been learned over the past five years. The receptors have been purified to homogeneity by affinity chromatography, have been reconstituted into phospholipid vesicles together with G-proteins, and the effector enzyme adenylyl cyclase and the various receptor functions probed in such reconstituted systems Limited protein sequence information, obtained from peptides derived from the purified receptors, has permitted the design and use of oligonucleotide probes that have led to the successful cloning of the gene and cDNA for the receptor, thus, permitting deduction of the complete amino acid sequence. This in turn has opened the way for the cloning of the genes for all the other adrenergic receptors, and for a variety of other receptors coupled to guanine nucleotide regulatory proteins.

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Authors
  1. Marc G. Caron
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  2. Robert J. Lefkowitz
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Editors and Affiliations

  1. Southwestern Medical Center at Dallas, The University of Texas, Dallas, Texas, USA

    John P. Perkins

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Caron, M.G., Lefkowitz, R.J. (1991). Structure-Function Relationships. In: Perkins, J.P. (eds) The Beta-Adrenergic Receptors. The Receptors. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0463-3_2

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  • DOI: https://doi.org/10.1007/978-1-4612-0463-3_2

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