The Neurotensin Receptor from Mammalian Brain
Neurotensin is a peptide of 13 amino acids (pGlu-Leu-Tyr-Glu-AsnLys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu) that is mainly localized in the central nervous system (Carraway and Leeman, 1973) and in the gastrointestinal tract (Kitabgi et al., 1976). A number of pharmacological, biochemical, and histochemical data suggest that neurotensin works as a neurotransmitter or neuromodulator in the brain (Nemeroff et al., 1982) and as a hormone in the periphery (Hirsh Fernstrom et al., 1980). Both modes of action imply as a first step the selective interaction of the peptide with specific receptors located on the plasma membrane of target cells. The structural and functional properties of neurotensin binding sites have been characterized in a number of tissue preparations and cell cultures of neural or nonneural origin (Kitabgi et al., 1985). Some of these sites represent functional neurotensin receptors since they are coupled to GTP-binding proteins and regulate intracellular levels of second messengers such as cAMP, cGMP, and inositol phosphates (Bozou et al., 1986; Amar et al., 1985; Gilbert et al., 1986; Goedert et al., 1984; Amar et al., 1986,1987).
KeywordsMouse Brain Affinity Column Inositol Phosphate Soluble Receptor Scatchard Plot
Unable to display preview. Download preview PDF.
- Checler. F., Vincent, J. P., and Kitabgi, P. (1986a) J. Biol. Chem. 261,11274–11281.Google Scholar
- Hirsch Fernstrom, M., Carraway, R. E., and Leeman, S. E. (1980) in Frontiers in Neuroendocrinology, vol 6, (Martini, L. and Ganong, W. F., eds.), Raven, New York, pp. 103–127.Google Scholar
- Kitabgi, P., Checler, F., Mazella, J., and Vincent, J. P. (1985) Reviews in Basic and Clinical Pharmacology 5, 397–484.Google Scholar
- Nemeroff, C. B., Luttinger, D., and Prange, Jr., A. J. (1982) Handbook of Psychopharmacology 16, 363–467.Google Scholar