The Neurotensin Receptor from Mammalian Brain

Solubilization and Purification by Affinity Chromatography
  • Jean-Pierre Vincent
  • Jean Mazella
  • Joëlle Chabry
  • Nicole Zsurger
Part of the Receptor Purification book series (RP, volume 1)

Abstract

Neurotensin is a peptide of 13 amino acids (pGlu-Leu-Tyr-Glu-AsnLys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu) that is mainly localized in the central nervous system (Carraway and Leeman, 1973) and in the gastrointestinal tract (Kitabgi et al., 1976). A number of pharmacological, biochemical, and histochemical data suggest that neurotensin works as a neurotransmitter or neuromodulator in the brain (Nemeroff et al., 1982) and as a hormone in the periphery (Hirsh Fernstrom et al., 1980). Both modes of action imply as a first step the selective interaction of the peptide with specific receptors located on the plasma membrane of target cells. The structural and functional properties of neurotensin binding sites have been characterized in a number of tissue preparations and cell cultures of neural or nonneural origin (Kitabgi et al., 1985). Some of these sites represent functional neurotensin receptors since they are coupled to GTP-binding proteins and regulate intracellular levels of second messengers such as cAMP, cGMP, and inositol phosphates (Bozou et al., 1986; Amar et al., 1985; Gilbert et al., 1986; Goedert et al., 1984; Amar et al., 1986,1987).

Keywords

Cholesterol Lithium Fluoride Electrophoresis Prolinal 

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References

  1. Amar, S., Mazella, J., Checler, F., Kitabgi, P., and Vincent, J. P. (1985) Biochem. Biophys. Res. Commun. 129,117–125.PubMedCrossRefGoogle Scholar
  2. Amar, S., Kitabgi, P., and Vincent, J. P. (1986) FEES Lett. 201, 31–36.CrossRefGoogle Scholar
  3. Amar, S., Kitabgi, P., and Vincent, J. P. (1987) J. Neurochem. 49, 999–1006.PubMedCrossRefGoogle Scholar
  4. Bozou, J. C., Amar, S., Vincent, J. P., and Kitabgi, P. (1986) Mol. Pharmacol. 29,489–496.PubMedGoogle Scholar
  5. Carraway, R. E. and Leeman, S. E. (1973) J. Biol. Chem. 248, 6854–6861.PubMedGoogle Scholar
  6. Checler. F., Vincent, J. P., and Kitabgi, P. (1986a) J. Biol. Chem. 261,11274–11281.Google Scholar
  7. Checler, F., Mazella, J., Kitabgi, P., and Vincent, J. P. (1968b) J. Neurochem. 47,1742–1748.CrossRefGoogle Scholar
  8. Gilbert, J. A., Moses, C. J., Pfenning, M. A., and Richelson, E. (1986) Biochem. Pharmacol. 35, 391–397.PubMedCrossRefGoogle Scholar
  9. Goedert, M., Pinnock, R. D., Downes, C. P., Mantyh, P. W., and Emson, P. C. (1984) Brain. Res. 323,193–197.PubMedCrossRefGoogle Scholar
  10. Hirsch Fernstrom, M., Carraway, R. E., and Leeman, S. E. (1980) in Frontiers in Neuroendocrinology, vol 6, (Martini, L. and Ganong, W. F., eds.), Raven, New York, pp. 103–127.Google Scholar
  11. Kitabgi, P., Carraway, R. E., and Leeman, S. E. (1976) J. Biol. Chem. 251, 7053–7058.PubMedGoogle Scholar
  12. Kitabgi, P., Checler, F., Mazella, J., and Vincent, J. P. (1985) Reviews in Basic and Clinical Pharmacology 5, 397–484.Google Scholar
  13. Mazella, J., Poustis, C., Labbé, C., Checler, F., Kitabgi, P., Granier, C., Van Rietschoten, J., and Vincent, J. P. (1983) J. Biol. Chem. 258, 3476–3481.PubMedGoogle Scholar
  14. Mazella, J., Chabry, J., Kitabgi, P., and Vincent, J. P. (1988) J. Biol. Chem. 263,144–149.PubMedGoogle Scholar
  15. Nemeroff, C. B., Luttinger, D., and Prange, Jr., A. J. (1982) Handbook of Psychopharmacology 16, 363–467.Google Scholar
  16. Poustis, C., Mazella, J., Kitabgi, P., and Vincent, J. P. (1984) J. Neurochem. 42,1094–1100.PubMedCrossRefGoogle Scholar
  17. Sadoul, J. L., Mazella, J., Amar, S., Kitabgi, P., and Vincent, J. P. (1984) Biochem. Biophys. Res. Commun. 120, 812–819.PubMedCrossRefGoogle Scholar
  18. Schotte, A., Leysen, J. E., and Laduron, P. M. (1986) Naunyn-Schmiedebergs Arch. Pharmacol. 333, 400–405.CrossRefGoogle Scholar
  19. Schotte, A. and Laduron, P. M. (1987) Brain Res. 408, 326–328.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Jean-Pierre Vincent
  • Jean Mazella
  • Joëlle Chabry
  • Nicole Zsurger

There are no affiliations available

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