Skip to main content
SpringerLink
Log in

Effects of Ionizing Radiation on Human Papillomavirus Immortalized Human Bronchial Epithelial Cells

  • Chapter
Neoplastic Transformation in Human Cell Culture

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 25))

  • 72 Accesses

Abstract

The carcinogenic action of ionizing radiation in humans has been well recognized from epidemiologic data. Despite this fact there has been only one report on the radiogenic transformation of human epithelial cells (1). We have established immortalized, non-tumorigenic human bronchial epithelial cell lines following transfection with human papillomaviruses (HPV) 16 or 18 (2) and are employing them in experiments designed to identify genetic mechanisms involved in neoplastic transformation of human bronchial epithelial cells by ionizing radiation. The specific investigations are 1) analysis for specific genetic alterations in transformants (cells that have altered morphology, growth in soft agar or tumorigenicity in immunosuppressed mouse); and 2) identification of mechanisms involved in differentiation of human bronchial epithelial cells by evaluation of the differentiation-specific transcription of the E6/E7 transforming genes in radiated cells.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Thraves, P., Salehi, Z. et al. Proc. Natl. Acad. Sci. U.S.A. 87, 1174 (1990).

    Article  PubMed  CAS  Google Scholar 

  2. Willey, J.C., Bressoud, A. et al, Cancer Research, in press, 1991..

    Google Scholar 

  3. Cook, P.J., Doll, R., Fellingham, S.A. International Journal of Cancer 4, 93 (1969).

    Article  CAS  Google Scholar 

  4. Willey, J. and Harris, C.C. CRC Critical reviews in Oncology/Hematology 10, 18 (1990).

    Google Scholar 

  5. Hunter, T. Cell 64, 249 (1991).

    Article  PubMed  CAS  Google Scholar 

  6. Bos, J.L. Mutation Research 195, 255 (1988)

    Article  PubMed  CAS  Google Scholar 

  7. Hollstein, M.M., et al., Science 253, 49 (1991).

    Article  PubMed  CAS  Google Scholar 

  8. Harbour, J.W., Lai, S.-L., et al. Science 353, 241 (1988).

    Google Scholar 

  9. Barbosa, M.S. and Schlegel, R. Oncogene 4, 1529 (1989).

    PubMed  CAS  Google Scholar 

  10. Stoler, M.H., Wolinsky, S.M., et al. Virology 172, 331 (1989).

    Article  PubMed  CAS  Google Scholar 

  11. Breitbart, R.E., Nguyen, H.T., et al. Cell 41, 67 (1985).

    Article  PubMed  CAS  Google Scholar 

  12. Willey, et al. Carcinogenesis 5, 209 (1984).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Environmental Health Sciences Center and Departments of Biophysics, Pediatrics and Biostatistics, University of Rochester School of Medicine and Dentistry, Rochester, NY, 14642, USA

    James C. Willey, Jim Greene, Alberec Bressoud, Peter Cerutti, Tom Hei, Nancy Wang, David Maillie, Chris Cox & Ellen Miles

  2. Center for Radiobiological Research, Columbia University, New York, NY, 10032, USA

    James C. Willey, Jim Greene, Alberec Bressoud, Peter Cerutti, Tom Hei, Nancy Wang, David Maillie, Chris Cox & Ellen Miles

  3. Department of Carcinogenesis, Swiss Institute for Experimental Cancer Research, Epalinges S./Lausanne, CH-1066, Switzerland

    James C. Willey, Jim Greene, Alberec Bressoud, Peter Cerutti, Tom Hei, Nancy Wang, David Maillie, Chris Cox & Ellen Miles

Authors
  1. James C. Willey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jim Greene
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alberec Bressoud
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter Cerutti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tom Hei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nancy Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David Maillie
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chris Cox
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ellen Miles
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD, USA

    Johng S. Rhim

  2. Department of Radiation Medicine, Georgetown University Medical Center, Washington DC, USA

    Anatoly Dritschilo

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Willey, J.C. et al. (1991). Effects of Ionizing Radiation on Human Papillomavirus Immortalized Human Bronchial Epithelial Cells. In: Rhim, J.S., Dritschilo, A. (eds) Neoplastic Transformation in Human Cell Culture. Experimental Biology and Medicine, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0411-4_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-0411-4_11

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6750-8

  • Online ISBN: 978-1-4612-0411-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation