Abstract
The Burkitt’s lymphoma cell line, Akata, demonstrates prompt and synchronous activation of latent EBV genomes following cross-linking of its membrane immunoglobulin G (mIgG) with anti-IgG (1). In B cells, activation from crosslinkage of mlg is initiated by a mlg-triggered phospholipase C (PLC), which cleaves phosphatidylinositol (PI) to inositol-1,4,5 trisphosphate (IP3) and diacylglycerol (DAG) (Fig. 1). IP3releases intracellular Ca++which can activate Ca++/calmodulin-dependent protein kinases, and DAG activates protein kinase C (PKC) (2). Adenylate cyclase can be activated, yielding cAMP which regulates protein kinase A (PKA). We have examined EBV activation in the Akata cells, the kinetics and synergy of these second messengers and the effect of cAMP, in the context of early antigen (EA) induction after anti-IgG crosslinking of mIgG (3).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Takada, K., and Y. Ono. J. Virol. 63, 445 (1989).
Coggeshall, K. M., and J. C. Cambier. J. Immunol. 133, 3382 (1984).
Daibata, M., R. E. Humphreys, K. Takada, and T. Sairenji. J. Immunol. 144, 4788 (1990).
Weiss, B., W. Prozialeck, M. Cimino, M. S. Barnette, and T. L. Wallace. Ann. N.Y. Acad. Sci. 356, 319 (1980).
Nishizuka, Y. Nature 308, 693 (1984).
Tamaoki, T., H. Nomoto, I. Takahashi, Y. Kato, M. Morimoto, and F. Tomita. Biochem. & Biophys. Res. Comm. 135, 397 (1986).
Seamon, K. B., W. Padgett, and J. W. Daly. Proc. Natl. Acad. Sci. USA 78, 3363 (1981).
Kaibuchi, K., Y. Takai, Y. Ogawa, S. Kimura, and Y. Nishizuka. Biochem. Biophys. Res. Commun. 104, 105 (1982).
Kenney, S., J. Kamine, E. Holley-Guthrie, J-C. Lin, E-C. Mar, and J. Pagano. J. Virol. 63, 1729 (1989).
Takada, K., N. Shimizu, S. Sakuma, and Y. Ono. J. Virol. 57, 1016 (1986).
Countryman, J., H. Jenson, R. Seibl, H. Wolf, and G. Miller. J. Virol. 61, 3672 (1987).
Deutsch, P. J., J. P. Hoeffler, J. L. Jameson, and J. F. Habener. Proc. Natl. Acad. Sci. USA 85, 7922 (1988).
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Daibata, M., Takagi, S., Humphreys, R.E., Takada, K., Sairenji, T. (1991). EBV Activation By Anti-IgG-Triggered, Second Messenger Pathways. In: Ablashi, D.V., Huang, A.T., Pagano, J.S., Pearson, G.R., Yang, C.S., Ablashi, K.L. (eds) Epstein-Barr Virus and Human Disease · 1990. Experimental Biology and Medicine, vol 24. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0405-3_45
Download citation
DOI: https://doi.org/10.1007/978-1-4612-0405-3_45
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-6747-8
Online ISBN: 978-1-4612-0405-3
eBook Packages: Springer Book Archive