Relationship of the EBV Lytic Activator Zta to the bZIP Family of Cellular Transactivators
Part of the
Experimental Biology and Medicine
book series (EBAM, volume 24)
The Zta (BZLF1) transactivator is capable of reactivating lytic cycle expression when introducted into latently infected B-cells (1) and hence represents the pivotal viral protein in the reactivation process. In earlier studies we utilized a bacterially expressed Zta in DNAseI footprinting assays to identify six Zta binding sites in the BamHI-H divergent promoter (2). The sequences protected by Zta binding were TGTGTAA, TGAGCAA, TGTGCAA and TGTGTCA (3 copies). These sites resemble the AP-1 binding site (TGAGTCA) for the Jun/Fos cellular transcription factors and Zta binds equally well to an AP-1 site as a ZRE site (2,3). The sites identified not only bind Zta but when cloned into a normally non-responsive target, for example TK-CAT or A10-CAT, confer Zta responsiveness on these targets (2).
KeywordsLeucine Zipper Hybrid Protein Basic Domain Dimerization Domain Heptad Repeat
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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