Relationship of the EBV Lytic Activator Zta to the bZIP Family of Cellular Transactivators

  • Y.-N. Chang
  • G. S. Hayward
  • S. D. Hayward
Part of the Experimental Biology and Medicine book series (EBAM, volume 24)


The Zta (BZLF1) transactivator is capable of reactivating lytic cycle expression when introducted into latently infected B-cells (1) and hence represents the pivotal viral protein in the reactivation process. In earlier studies we utilized a bacterially expressed Zta in DNAseI footprinting assays to identify six Zta binding sites in the BamHI-H divergent promoter (2). The sequences protected by Zta binding were TGTGTAA, TGAGCAA, TGTGCAA and TGTGTCA (3 copies). These sites resemble the AP-1 binding site (TGAGTCA) for the Jun/Fos cellular transcription factors and Zta binds equally well to an AP-1 site as a ZRE site (2,3). The sites identified not only bind Zta but when cloned into a normally non-responsive target, for example TK-CAT or A10-CAT, confer Zta responsiveness on these targets (2).


Leucine Zipper Hybrid Protein Basic Domain Dimerization Domain Heptad Repeat 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Y.-N. Chang
    • 1
  • G. S. Hayward
    • 1
  • S. D. Hayward
    • 2
  1. 1.Departments of Pharmacology and Molecular SciencesThe Johns Hopkins School of MedicineBaltimoreUSA
  2. 2.NeurologyThe Johns Hopkins School of MedicineBaltimoreUSA

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