Abstract
An EBV-specific alkaline deoxyribonuclease (DNase) activity was first demonstrated in a few lymphoblastoid cell lines which containing both endonucleolytic and exonucleolytic activities (1,2). This enzyme acitivity was also detected in Raji cells superinfected with the virus (3). A retrospective study and a prospective study demonstrated that the anti-EBV DNase antibody in NPC patients araised before the appearance of clinical symptoms (4,5). In previous studies, we have identified a cDNA clone, BG9, from IUdR treated P3HR1 cells which encodes EBV specific DNase (6). When this 1.8 kb clone was used as a probe, 4 EBV DNase related transcripts have been revealed in P3HR1 cells by Northern analysis. In this study, the exact initiation and termination sites of the P3HR1 EBV DNase related transcripts were anaylsed.
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References
Clough, W. Biochemistry 18,4517–4521 (1979).
Clough, W. Proc. Natl. Acad. Sci. USA 77,6194–6198 (1980).
Cheng Y G, Chen J Y, Hoffman P J, and Glaser, R. Virol. 100,334–338 (1980).
Chen J Y, Hwang L Y, Beasley R P, Chien C S, and Yang C S. J. Med. Virol 16,99–105 (1985).
Chen J Y, Chen C J, Liu M Y, Cho S M, Hsu M M, Lynn T C, Shieh T, Tu S M, Beasley R P, Hwang L Y, Lee H H, Kou S L, and Yang C S. J. Med. Virol. 16,99–105. (1989).
Chen M R, Hsu T Y, Chen J Y, and Yang C S. The Epstein-Barr Virus and Human Disease (The Humana Press).pp 69–74 (1988).
Chirgwin I M, Przybyla A E, MacDonald R J and Rutter W J. Biochemistry 18,5294–5299 (1979).
Thomas P S. Methods Enzymo1.100,255–266 (1983).
Berk A J and Sharp P A. Nature(London) 310,207–211. (1977)
Sanger F, Nicklens and Coulson A. R.Proc. Natl. Acad Sci. USA 74,5463–5467 (1977).
Costa R H, Draper K G, Banks L, Powell k L, Chen G, Eisenberg, R and Wanger E. K. J Virol 48, 591–603 (1983).
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© 1991 Springer Science+Business Media New York
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Chen, M.R., Hsu, T.Y., Chen, J.Y., Yang, C.S. (1991). Characterization of EBV DNase Related Transcripts. In: Ablashi, D.V., Huang, A.T., Pagano, J.S., Pearson, G.R., Yang, C.S., Ablashi, K.L. (eds) Epstein-Barr Virus and Human Disease · 1990. Experimental Biology and Medicine, vol 24. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0405-3_12
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DOI: https://doi.org/10.1007/978-1-4612-0405-3_12
Publisher Name: Humana Press, Totowa, NJ
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