Tumor-Marker Detection by Isotachophoresis in Porous Membranes

Bence Jones Protein in Chronic Lymphocytic Leukemia and Non-Hodgkin’s Lymphoma
  • Garri I. Abelev
  • Eleonora R. Karamova
Part of the Contemporary Biomedicine book series (CB, volume 11)

Abstract

Isotachophoresis (ITP) is a very powerful electrophoretic method for separation of native macromolecules as well as substances of low molecular weight (Haglund, 1970; Chrambach, 1980). ITP combines very effective concentration of the substances with their sharp separation according to electrophoretic mobility of each component. Since ITP is hardly compatible with electroosmosis, it is usually carried out in free medium, as in capillary electrophoresis, or in gels lacking electroosmotic properties. We have modified ITP using highly “electroosmotic” cellulose acetate (CAM) as a supporting medium (Abelev and Karamova, 1982, 1984). This modification (ITP-CAM) has two major advantages over “standard” ITP:
  1. 1.

    ITP-CAM exploits electroosmotic flow in CAM to create very effective counterflow, which completely equilibrates the electrophoretic migration of the separated components. This permits the use for analysis of highly diluted solutions of proteins or other macromolecules, which are transferred by counterflow to the ITP area, concentrated, and separated in this area.

     
  2. 2.

    The concentrated and separated bands of macromolecules on CAM can be easily detected by different immunochemical methods: immunodiffusion, immunoelectrophoresis, immunofixation, or immunoblotting. Hence, ITP-CAM is an extremely useful technique for analysis of low-protein biological fluids, such as urine, cerebrospinal and amniotic fluids, tears, saliva, and so on.

     

Keywords

Cellulose Migration Albumin Agar Lymphoma 

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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Garri I. Abelev
  • Eleonora R. Karamova

There are no affiliations available

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