Tumor-Marker Detection by Isotachophoresis in Porous Membranes
ITP-CAM exploits electroosmotic flow in CAM to create very effective counterflow, which completely equilibrates the electrophoretic migration of the separated components. This permits the use for analysis of highly diluted solutions of proteins or other macromolecules, which are transferred by counterflow to the ITP area, concentrated, and separated in this area.
The concentrated and separated bands of macromolecules on CAM can be easily detected by different immunochemical methods: immunodiffusion, immunoelectrophoresis, immunofixation, or immunoblotting. Hence, ITP-CAM is an extremely useful technique for analysis of low-protein biological fluids, such as urine, cerebrospinal and amniotic fluids, tears, saliva, and so on.
KeywordsCellulose Migration Albumin Agar Lymphoma
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